Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. These two isoforms are transcribed from distinct estrogen receptor (ER)-inducible promoters within a single copy PR gene. Clone 16 is specific for a region of the N-terminus of the A form of PR. The precise epitope has not been mapped but it reacts with both A and B forms of PR by Western blot but only with the A form by immunohistochemistry. This suggests that the epitope is inaccessible in the native folded B form of the protein.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
16
Concentration:
Greater than or equal to 324 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Hungermann D et al. Journal of Pathology 2002; 198: 487494
The progesterone receptor (PgR) is an estrogen regulated protein. It has been proposed that expression of PgR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PgR determination provides supplementary information to ER, both in predicting response to endocrine therapy and estimating survival. PgR has proved superior to ER as a prognostic indicator in some studies. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tisse Breast, breast ducatl carcinoma. Staining Nuclear
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR4
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
Epidermal growth factor receptor (EGFR) is a transmembrane protein receptor of 170 kD with tyrosine kinase activity. Increased levels of EGFR are reported to be linked with malignant transformation of squamous cells eg in squamous cell carcinoma of the lung, head, neck, skin, cervix and esophagus. EGFR may also play a role in the development and progression of hepatocellular carcinomas where recurrence rates are higher in EGFR-positive cases. This correlation has similarly been reported in colorectal cancers where EGFR, produced by tumor cells, plays an important role in the invasiveness and proliferation of colorectal cancers. The majority of published studies of EGFR expression in human breast cancer has similarly shown an association with EGFR expression where it is inversely related to estrogen receptor status.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
EGFR.113
Concentration:
Greater than or equal to 26 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Lodge AJ et al. Journal of Clinical Pathology. 2003; 56(4):300304
References 2:
Sriplakich S et al. BJU Int. 1999; 83(4):498503
References 3:
Inoue K et al. Acta Med Okayama 1998; 52(6):305310
References 4:
Tungekar MF and Linehan J. Journal of Clinical Pathology. 1998; 51:583587
Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Breast carcinoma. Staining nuclear.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR2
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. The introduction of highly specific monoclonal antibodies to ER has allowed the determination of receptor status of breast tumors to be carried out in routine histopathology laboratories.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
6F11
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Bevitt DJ et al. Journal of Pathology 1997; 183(2), 228232
References 2:
Kaplan, PA et al. Am J Clin Pathol 2005: 276280
References 3:
Zafrani B et al. Histopathology 2000; 37(6), 536545
References 4:
Harvey JM et al. Journal of Clinical Oncology 1999; 17(5), 14741481
References 5:
Khan SA et al.European Journal of Cancer 2000; 36(Suppl 4), S27S28
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Polo-Like Kinase-1 (PLK1) also known as Serine/Threonine Protein Kinase 13 is a 66 kDa kinase. The activity of PLK-1 is crucial for mitosis and maintenance of genome stability. PLK-1 localizes to centrosomes and kinetochores where it plays a key role in late prophase and prometaphase. PLK-1 is overexpressed in many types of cancers and mediates estrogen receptor-mediated gene transcription in breast cancer cells. Overexpression of PLK-1 is associated with tumor development, with elevated levels of expression reported in non-small cell lung cancers, head and neck, gastric, breast, ovarian, colon and several other cancer types.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
MJS1
Concentration:
Greater than or equal to 320 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
Cookies:
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