Agrisera

Agrisera is a specialist manufacturer of primary antibodies with a particular emphasis on antibodies for plant science.

Their plant antibody range includes antibodies against algal and plant antigens with a particular focus on plant cellular compartments (Chloroplast markers, Cytoplasm markers, ER markers, Golgi markers, Mitochondrial markers, Nuclear markers, Peroxisomal markers, Plasma membrane markers, Plastidial markers, Vacuolar markers), antibodies for research in environmental stress, nitrogen metabolism and photosynthesis.

Agrisera also produce antibodies against bacterial, fungal and insect antigens as well as a wide range of human antigens including several antibodies for use in research on Alzheimer's disease (including Beta Amyloid antibodies), Parkinson's disease (Alpha-synuclein) and diabetes type II (Amylin or Islet Amyloid Polypeptide (IAPP)) . Also available are a set of antibodies to salmonid stress proteins.

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Agrisera have developed a range of antibodies for the study of Nitrogen Metabolism in plants and algae.

Available antibodies include glutamine oxoglutarate aminotransferase (GOGAT) and Glutamine Synthetase (GlnA) including a global GLN antibody, which can be used across a range of different species.

This growing range of antibodies are available in single vials or in larger volumes offering lower pricing.

Click here for the full list of nitrogen metabolism antibodies. 

The rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. It contains anti-RbcL antibody, recombinant RbcL protein and the Agrisera PEB buffer for quantitative protein extraction.

The kit contains

►rabbit polyclonal antibody to RbcL AS03 037, quantity | 100 µg amount enough for 100 Western blots

►Rubisco (RbcL) recombinant protein standard AS01 017S, quantity | 250 µl at 0.15 pmoles/µl, amount enough for generation of standard curve in 85 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)

►Agrisera PEB extraction buffer AS08 300, quantity | 3 x 2 ml (4x stock), amount enough for 48 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 ug total chlorophyll)

►manual

Application Example

2 µg of total protein from various plant extracts  (1-5) extracted with PEB (AS08 300)  separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Markers MagicMarks (Invitrogen) (M) and  Rubisco protein standard (AS01 017S) at 0.0625 pmol, 0.125 pmol, 0.25 pmol.

Following standard western blot procedure this image has been obtained using a CCD imager and Quantity One software.  The contour tool of the software is used to select the area for quantitation and the values are background subtracted to give an adjusted volume in counts for each standard and sample.

Click here for more information on the Rubisco Quantitation Kit and related Rubisco antibodies.