DNA polymerase, DNA ladders, nucleic acid stains and related reagents for molecular biology
Newmarket Scientific provides both general and novel molecular biology reagents with a focus on the following areas:
Also The full list of our Molecular Biology reagents is available here if what you are looking for does not come under any of the categories above or alternatively you can simply complete our contact form and we'll look for you.
Novel Juice is a non-mutagenic alternative to Ethidium bromide (shown by independent testing). It fluoresces brightly under Blue Light or UV illumination and can be used with nucleic acid markers and samples being run on agarose or polyacrylamide gels.
We believe Novel Juice to be the most sensitive stain available for detecting double-stranded DNA (dsDNA). It also contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visual tracking DNA migration on your gel without illumination.
Plus it is much less expensive than other Ethidium Bromide Alternatives.
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
1ml of 6x solution £35 (Cat No LD001-1000)
10 x 1ml of 6x solution £300 (Cat No LD001-1000x10)
If you would like further information, a copy of the mutagenicity testing report for Novel Juice or a free sample please use our contact form.
Using Novel Juice:
(1) Vortex Novel Juice for 10 seconds prior to use.
(2) Dilute 1 part Novel Juice with 5 parts DNA sample or ladder and mix.
(3) Load sample and run according to your standard lab procedures.
(4) After electrophoresis, visualise with a UV or visible-light transilluminator
(5) Gels can also be post-stained with Ethidium Bromide if desired.
Storage Temp: RT or 4°C for 12 months
The M-MLV RT RNase H- is a genetically modified M-MLV Reverse Transcriptase, which lacks the ribonuclease H activity of normal M-MLV.
It synthesises a complementary DNA strand initiating from a primer using RNA (or single stranded DNA) as a template.
Most importantly removal of the RNase H activity results in an increase of full-length cDNA products.