Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a <a href="https://www.bosterbio.com/cells/reporter-cell-lines" style="color:#ea8d28">reporter gene</a> in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity. Subcellular Localization: Tissue Specificity:
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and is rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and HER2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumours, therefore Anti-GATA3 is useful for carcinoma diagnosis when those of the breast and bladder are plausible.
Epidermal growth factor receptor (EGFR) is a transmembrane protein receptor of 170 kD with tyrosine kinase activity. Increased levels of EGFR are reported to be linked with malignant transformation of squamous cells eg in squamous cell carcinoma of the lung, head, neck, skin, cervix and esophagus. EGFR may also play a role in the development and progression of hepatocellular carcinomas where recurrence rates are higher in EGFR-positive cases. This correlation has similarly been reported in colorectal cancers where EGFR, produced by tumor cells, plays an important role in the invasiveness and proliferation of colorectal cancers. The majority of published studies of EGFR expression in human breast cancer has similarly shown an association with EGFR expression where it is inversely related to estrogen receptor status.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
EGFR.113
Concentration:
Greater than or equal to 26 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Lodge AJ et al. Journal of Clinical Pathology. 2003; 56(4):300304
References 2:
Sriplakich S et al. BJU Int. 1999; 83(4):498503
References 3:
Inoue K et al. Acta Med Okayama 1998; 52(6):305310
References 4:
Tungekar MF and Linehan J. Journal of Clinical Pathology. 1998; 51:583587
Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. The introduction of highly specific monoclonal antibodies to ER has allowed the determination of receptor status of breast tumors to be carried out in routine histopathology laboratories.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
6F11
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Bevitt DJ et al. Journal of Pathology 1997; 183(2), 228232
References 2:
Kaplan, PA et al. Am J Clin Pathol 2005: 276280
References 3:
Zafrani B et al. Histopathology 2000; 37(6), 536545
References 4:
Harvey JM et al. Journal of Clinical Oncology 1999; 17(5), 14741481
References 5:
Khan SA et al.European Journal of Cancer 2000; 36(Suppl 4), S27S28
1011 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1011
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1014 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1014
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1027 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1027
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Antibody Isotype:
IgM
Monosan Range:
MONOSAN
Clone:
1032
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Abbondanza, C. et al, J. Cell Biol. 141, 1301-1310 (1998)
Polo-Like Kinase-1 (PLK1) also known as Serine/Threonine Protein Kinase 13 is a 66 kDa kinase. The activity of PLK-1 is crucial for mitosis and maintenance of genome stability. PLK-1 localizes to centrosomes and kinetochores where it plays a key role in late prophase and prometaphase. PLK-1 is overexpressed in many types of cancers and mediates estrogen receptor-mediated gene transcription in breast cancer cells. Overexpression of PLK-1 is associated with tumor development, with elevated levels of expression reported in non-small cell lung cancers, head and neck, gastric, breast, ovarian, colon and several other cancer types.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. These two isoforms are transcribed from distinct estrogen receptor (ER)-inducible promoters within a single copy PR gene. Clone 16 is specific for a region of the N-terminus of the A form of PR. The precise epitope has not been mapped but it reacts with both A and B forms of PR by Western blot but only with the A form by immunohistochemistry. This suggests that the epitope is inaccessible in the native folded B form of the protein.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
16
Concentration:
Greater than or equal to 324 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Hungermann D et al. Journal of Pathology 2002; 198: 487494
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