p63 consists of two major isoforms -TAp63 and ?Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation- competent TAdomain with homology to p53, which regulates the expression of the growth -inhibitor y genes. In contrast, ?Np63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally- inactive ?N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) recognizes exclusively ?Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma v s. invasive breast ductal carcinoma. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Lung squamous cell carcinoma. Staning nuclear
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR8
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody in 0.2% BSA and 15mM sodium azide.
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
For the development of sandwich ELISA kit to measure mouse Oncostatin M/OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Product Type:
Antibodies Primary
Storage Temp:
-20°C
Immunogen:
Expression system for standard: E.coli; Immunogen sequence: A24-R206
Applications:
ELISA
Additional Info:
For the development of sandwich ELISA kit to measure mouse Oncostatin M/OSM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
The accumulation of p53 protein in response to genotoxic stress in vitro is well established and appears to induce growth arrest and apoptosis by the transcriptional regulation of other genes and possibly by other direct mechanisms.
Monosan Range:
MONXtra
Concentration:
n/a
Storage buffer:
PBS with 1% BSA and sodium azide
Storage:
2-8°C
References 1:
Botchkarev VA et al. American Journal of Pathology. 158 (6): 19131919 (2001)
References 2:
Midgley CA et al. Journal of Cell Science. 108: 18431848 (1995
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Breast, Prostate, Prostate carcinoma or lung or bladder squamous cell carcinoma
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
TP63/11
Concentration:
n/a
Format:
Concentrate
Storage buffer:
Bioreactor Concentrate with 0.05% Azide
Storage:
2-8°C
References 1:
Yang A, et al. Mol Cell 1998;2:305-16
References 2:
Signoretti S, et al Am J Pathol 2000;157:1769-75
References 3:
Yang A, et al. Nature 1999;398:714-18
References 4:
Barbareschi M. et al. Am J Surg Pathol 2001 Aug;25(8);1054-60
References 5:
Werling RW, et al. Am J Surg Pathol 2003 Jan;27(1):82-90
p21 is one of the inhibitors of the phosphorylation of the cyclin-cdk complex. p21, which is an inhibitor of G1 cdks, suppresses the cell cycle and inhibits DNA synthesis. Although p21 is induced by p53 and inhibits cdk (cyclin-dependent kinase) activity, there was virtually no correlation between the expression of p21 and that of p53; this finding was consistent with two reports, though another reported an inverse correlation between the expression of p21 and that of p53. p53independent expression of p21 might account for the discrepancy between the expression of p53 and that of p21. It is expressed in normal human tissue and a wide array of tumors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
DCS-60.2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN Ready To Use
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
The gene encoding WAF1, also termed p21, is transcriptionally regulated by the suppressor protein, p53. Overexpression of WAF1 is growth suppressive, possibly by inhibiting the activity of cyclin/CDK complexes. One consequence of WAF1 binding to cyclin/CDK is the inhibition of Rb protein phosphorylation. Induction of WAF1 expression requires wild type p53 activity in cells undergoing p53 dependent G1 arrest or apoptosis. Mutation of the p53 gene is a common event in human cancer and results in the failure to produce WAF1. The effect of this may lead to uncontrolled cell proliferation.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
4D10
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Göhring UJ et al. Journal of Clinical Pathology. 54: 866870 (2001)
References 2:
Schwerer MJ et al. Journal of Clinical Pathology. 54: 871876 (2001)
References 3:
Tweddle DA et al. American Journal of Pathology. 158 (6): 20672077 (2001)
References 4:
Garcia JF et al. Histopathology. 30: 120125 (1997)
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