The gene encoding WAF1, also termed p21, is transcriptionally regulated by the suppressor protein, p53. Overexpression of WAF1 is growth suppressive, possibly by inhibiting the activity of cyclin/CDK complexes. One consequence of WAF1 binding to cyclin/CDK is the inhibition of Rb protein phosphorylation. Induction of WAF1 expression requires wild type p53 activity in cells undergoing p53 dependent G1 arrest or apoptosis. Mutation of the p53 gene is a common event in human cancer and results in the failure to produce WAF1. The effect of this may lead to uncontrolled cell proliferation.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
4D10
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Göhring UJ et al. Journal of Clinical Pathology. 54: 866870 (2001)
References 2:
Schwerer MJ et al. Journal of Clinical Pathology. 54: 871876 (2001)
References 3:
Tweddle DA et al. American Journal of Pathology. 158 (6): 20672077 (2001)
References 4:
Garcia JF et al. Histopathology. 30: 120125 (1997)
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN Ready To Use
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
p21 is one of the inhibitors of the phosphorylation of the cyclin-cdk complex. p21, which is an inhibitor of G1 cdks, suppresses the cell cycle and inhibits DNA synthesis. Although p21 is induced by p53 and inhibits cdk (cyclin-dependent kinase) activity, there was virtually no correlation between the expression of p21 and that of p53; this finding was consistent with two reports, though another reported an inverse correlation between the expression of p21 and that of p53. p53independent expression of p21 might account for the discrepancy between the expression of p53 and that of p21. It is expressed in normal human tissue and a wide array of tumors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN
Clone:
DCS-60.2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The antibody recognizes a 53 kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with the mutant as well as wild type p53.1 Positive nuclear staining with this antibody has been shown to be a factor in breast carcinoma, lung carcinoma, colorectal carcinoma, urothelial carcinoma, and ependymoma.2-8 Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma, as well as a marker for intratubular germ cell neoplasia.
Antibody Isotype:
IgG2b-k
Monosan Range:
MONOSAN
Clone:
DO7
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Mauri FA et al. Int J Oncol 1999 Dec;15(6):1137-47
References 2:
Caffo O et al. Clin Cancer Res 1996 Sep;2(9):1591-9
References 3:
Bebenek M et al. Anticancer Res 1998 Jan-Feb;18(1B):619-23
References 4:
Midulla C et al. Anticancer Res 1999 Sep-Oct;19(5B):4033-7
References 5:
Moore BE et al. App.IHC and Mol. Morphol. 2001;9(3): 203 206
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