The expression of MDM2 is itself, induced by p53 and may be a way for p53 to self-regulate its activity during the normal cell cycle. However, overexpression of MDM2 results in the loss of p53-regulated growth control and consequently, deregulated cell proliferation. MDM2 also binds to the Retinoblastoma tumor suppressor protein (Rb) and inhibits its growth regulatory function. MDM2 can directly augment proliferation by binding to two transcription factors E2F1 and DP1 and stimulating the activity of the S-phase inducing E2F1/DP1 heterodimer. MDM2 migrates at a reduced molecular weight of ~95 kDa. The SMP14 clone has been reported to recognize human, mouse and rat MDM2 while exhibiting a slight cross-reactivity with cytokeratins 6, 14 and 16 in some experimental systems. In the immunoprecipitation application, SMP14 has been reported to precipitate MDM2 and p53-MDM2 complexes. MCF7 human breast carcinoma cells (ATCC HTB-22) and NIH/3T3 mouse fibroblasts (ATCC CRL-1658) are suggested as western blot and immunoprecipitation positive controls. SMP14 has been reported to be useful for the immunohistochemical staining of acetone-fixed, frozen sections and of formalin-fixed, paraffin-embedded tissue sections. In addition to a nuclear staining of MDM2, cytoplasmic staining may also be observed which is likely to be attributable to the slight cross reactivity of the SMP14 clone with cytokeratins. Control tisse Breat carcinoma. Staining Nuclear
p63 consists of two major isoforms -TAp63 and ?Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation- competent TAdomain with homology to p53, which regulates the expression of the growth -inhibitor y genes. In contrast, ?Np63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally- inactive ?N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) recognizes exclusively ?Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma v s. invasive breast ductal carcinoma. Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-rabbit secondary antibody-based detection is recommended. Control tissue Lung squamous cell carcinoma. Staning nuclear
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
ZR8
Concentration:
n/a
Format:
Purified
Storage buffer:
Purified antibody in 0.2% BSA and 15mM sodium azide.
The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63?, TAp63?, and TAp63?) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (?Np63?, ?Np63?, ?Np63?) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands.Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues
p53, also known as Tumour Protein 53 or TP53, is a tumour suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumours, including those of the breast, ovary, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
Anti-p40 recognizes squamous and basal cells, the shortest variant of p53, and ΔNp63 (an isoform of p63). p40 has been indicated as an alternative to p63 for the detection of Squamous Cell Carcinoma (SqCC), offering the advantage of eliminating potential misinterpretation of a positive adenocarcinoma as a SqCC.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC058
Antibody Isotype:
IgG1
GMDN Code:
64957
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Squamous Cell Carcinoma of Lung
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Mouse Double Minute 2 Homolog (MDM2), also known as HDM2 in humans, is crucial in negative regulation of the p53 tumour suppressor. Negative regulation is mediated through both the ubiquitination of p53/TP53, as well as inhibition of p53 transcriptional activation. Reports have indicated an overexpression of MDM2 to be associated with a number of different human tumour types, including soft tissue sarcomas, osteosarcomas, and breast tumours. When co-overexpressed with the CDK4 protein, MDM2 can also aid in the detection of well-differentiated liposarcomas and de-differentiated liposarcoma.
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Polyclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC012
Antibody Isotype:
IgG
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC002
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
RUO
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC042
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ClpB1 Chaperone protein ClpB1 is involved in acquired thermotolerance in cyanobacteria and belongs to molecular chaperones. Contributes to cold acclimation process in cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechococcus sp. strain PCC 7942
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
Immunogen:
C-terminal 163 amino acid sequence of the Synechococcus sp. strain PCC 7942 ClpB1 protein, fused to the MBP (maltose binding protein). UniProt: P53533
For western blot detection image refer to the article below
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
79 | 93 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Porankiewicz and Clarke (1997). Induction of the heat shock protein ClpB affects cold acclimation in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol. 1997 Aug;179(16):5111-7.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Chlamydomonas reinhardtii (too high background for this species)
Selected references:
Czernicka et al. (2022). Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming. Cells. 2022 Jan 31;11(3):500. doi: 10.3390/cells11030500. PMID: 35159309; PMCID: PMC8834170.Kumari et al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.Mishra, Sahu & Shaw (2021). Insight into the cellular and physiological regulatory modulations of Class-I TCP9 to enhance drought and salinity stress tolerance in cowpea. Physiol Plant. 2021 Aug 30. doi: 10.1111/ppl.13542. Epub ahead of print. PMID: 34459503.Zhuang et al (2021). EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis. Cell Rep. 2021 Jul 13;36(2):109384. doi: 10.1016/j.celrep.2021.109384. PMID: 34260941.Ngou et al. (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors. Nature. 2021 Mar 10. doi: 10.1038/s41586-021-03315-7. Epub ahead of print. PMID: 33692545.
Special application note:
Antibody available in 3 various pack sizes: 50, 100 and 150 l - Please inquire.This product can be sold containing ProClin if requested.
For the development of sandwich ELISA kit to measure mouse Oncostatin M/OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Product Type:
Antibodies Primary
Storage Temp:
-20°C
Immunogen:
Expression system for standard: E.coli; Immunogen sequence: A24-R206
Applications:
ELISA
Additional Info:
For the development of sandwich ELISA kit to measure mouse Oncostatin M/OSM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Biosite Brand:
BioSite ELISA
Species Reactivity:
mouse
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC042
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p63 is a tumour suppressor protein that is very similar to p53 in structure and function, while being homologous to p73. p63 is important in development and differentiation, and has been identified as a useful marker for distinguishing between lung squamous cell carcinomas and adenocarcinomas. Anti-p63 is also used to differentiate between benign and malignant prostate and breast lesions, due to its labeling of the nuclei of myoepithelial cells in both tissue types.
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors. SV40 is believed to suppress the transcriptional properties of tumor-suppressing p53 in humans through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumor suppressor p53, which is responsible for initiating regulated cell death (apoptosis), or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute to uncontrolled cellular proliferation, leading to a tumor.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-4
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Gurney, E.G., et al. J Virl. 34:752-763 (1980)
References 2:
Huang, H., Reis,R. et al. Brain Pathol., 9:33-42 (1999)
References 3:
Arrington, A.S., et al. Molecular and Clinical Perspectives; 461-489 (2001)
p53, also known as Tumour Protein 53 or TP53, is a tumour suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumours, including those of the breast, ovary, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Polyclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC012
Antibody Isotype:
IgG
GMDN Code:
57047
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Mouse Double Minute 2 Homolog (MDM2), also known as HDM2 in humans, is crucial in negative regulation of the p53 tumour suppressor. Negative regulation is mediated through both the ubiquitination of p53/TP53, as well as inhibition of p53 transcriptional activation. Reports have indicated an overexpression of MDM2 to be associated with a number of different human tumour types, including soft tissue sarcomas, osteosarcomas, and breast tumours. When co-overexpressed with the CDK4 protein, MDM2 can also aid in the detection of well-differentiated liposarcomas and de-differentiated liposarcoma.
Anti-p40 recognizes squamous and basal cells, the shortest variant of p53, and ΔNp63 (an isoform of p63). p40 has been indicated as an alternative to p63 for the detection of Squamous Cell Carcinoma (SqCC), offering the advantage of eliminating potential misinterpretation of a positive adenocarcinoma as a SqCC.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC058
Antibody Isotype:
IgG1
GMDN Code:
64957
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Squamous Cell Carcinoma of Lung
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
p21 is one of the inhibitors of the phosphorylation of the cyclin-cdk complex. p21, which is an inhibitor of G1 cdks, suppresses the cell cycle and inhibits DNA synthesis. Although p21 is induced by p53 and inhibits cdk (cyclin-dependent kinase) activity, there was virtually no correlation between the expression of p21 and that of p53; this finding was consistent with two reports, though another reported an inverse correlation between the expression of p21 and that of p53. p53independent expression of p21 might account for the discrepancy between the expression of p53 and that of p21. It is expressed in normal human tissue and a wide array of tumors.
Antibody Isotype:
IgG1-k
Monosan Range:
MONOSAN Ready To Use
Clone:
DCS-60.2
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC002
Antibody Isotype:
IgG2a
GMDN Code:
57047
UKCA Status:
RUO
CE-IVD Status:
RUO
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
