The basic structure of an immunoglobulin molecule consists of two identical heavy chains, either gamma, alpha, delta, or epsilon and two identical light chains, either kappa or lambda. Any heavy chain can associate with either light chain but on any immunoglobulin molecule both light chains are of the same type. The ratio of kappa and lambda light chains varies between Ig classes and subclasses. In a polyclonal population the ratio of kappa to lambda bearing B cells is approximately 2:1, with individual B cells thought to express kappa or lambda light chains, never both. The majority of kappa and lambda chains are bound to heavy chain immunoglobulin, however in normal individuals low levels of free light chain are present in serum. The occurrence of a mixture of kappa and lambda chain expressing cells suggests a polyclonal population and a reactive or non-neoplastic proliferation of B cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
SHL53
Concentration:
Greater than or equal to 554 mg/L
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Gertz M et al. Kidney International. 2002; 61(1):19
References 2:
Ramsland P and Farrugia W. Journal of Molecular Recognition. 2002; 15:248259
Immunoglobulins are polypeptides and comprise five major classes; immunoglobulin G (IgG), IgA, IgM, IgD and IgE. Each immunoglobulin consists of two identical heavy (H) chains and two identical light (L) chains.These are also subdivided into sub classes eg IgG1. There are two classes of light chain; kappa and lambda. The ratio of kappa chains and lambda chains varies between Ig classes and sub classes, but is also species specific. In humans, approximately 60 percent of light chains are kappa. However, in any particular immunoglobulin molecule the light chain will be either kappa or lambda. B cells contain either kappa or lambda mRNA.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
CH15
Concentration:
Greater than or equal to 125 mg/L
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Ramsland P and Farrugia W. Journal of Molecular Recognition. 2002; 15:248259
Folate is a basic component of cell metabolism and DNA synthesis and repair. It is involved in essential one-carbon transfer reactions and is a vitamin required by both normal and tumor cells. Folate entry into cells is facilitated via two different systems: the reduced folate carrier, which utilizes a bidirectional anion-exchange mechanism, and the folate receptor system. Folate receptor alpha is a membrane-bound member of the folate receptor family, facilitating folate transport via a mechanism termed potocytosis where the receptor is internalized and then recycled back to the cell membrane. Folate receptor alpha expression is reported to be highly restricted in normal tissues and only selectively overexpressed in a limited number of epithelial malignancies.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
BN3.2
Concentration:
Greater than or equal to 67 mg/L
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Smith AE et al. Hybridoma. 2007; 26(5):281288
References 2:
Kelemen L. International Journal of Cancer. 2006; 119:243250
IgM, together with IgD, is the major immunoglobulin expressed on the surface of B cells and normally constitutes about 10 per cent of serum immunoglobulin. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as natural blood group antibodies.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
8H6
Concentration:
Greater than or equal to 41 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Sangaletti S et al. Oncoimmunology. 2014; 3:e28989. doi: 10.4161/onci.28989
References 2:
Carsetti R et al. Immunological Reviews 2004; 197: 179-191
References 3:
Schaffer A et al. Nature Reviews: immunology. 2002; 2: 1-13
Mouse anti-Delta chain of human Immunoglobulin D, clone DRN1C (monoclonal)
Antibody Type:
Monoclonal
Host Animal:
Mouse
Species Reactivity:
human
Immunogen:
Prokaryotic recombinant protein corresponding to 222 amino acids of the N terminus of the delta heavy chain constant region of the human immunoglobulin D molecule.
IgD, together with IgM, are the major immunoglobulins expressed on the surface of B cells where it seems they may operate as mutually interacting antigen receptors for the control of lymphocyte activation and suppression. The greater susceptibility of IgD to proteolysis in combination with antigen could well be implicated in such a function. The use of PBS-based diluents may result in increased background staining. Clone DRN1C was developed to produce reduced background staining that is associated with polyclonal antibodies on paraffin sections.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
DRN1C
Concentration:
Greater than or equal to 133 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Geisberger R et al. Immunology. 2006; 118:429-437
References 2:
Preudhomme J et al. Molecular Immunology. 2000; 37:871-887
References 3:
Vladutiu A. Clinical and diagnostic laboratory immunology. 2000; 7(2):131-140
IgA is a member of the antibody class of the immunoglobulin superfamily. There are several classes and subclasses (isotypes) of antibody, the antibody isotype being defined by the immunoglobulin heavy chain present in the molecule. The basic structure of an immunoglobulin molecule consists of two identical heavy chains (gamma , mu, alpha , delta , epsilon) and two identical light chains, either kappa or lambda. IgA contains the alpha -chain and may be present in a serum or secretory form. In serum, 90% of IgA is monomeric, while in its secretory form it is the main immunoglobulin found in secretions including tears, saliva, intestinal and bronchial mucous, sweat, colostrum, and secretions from the prostate and respiratory epithelia, where it has the job of defending exposed external surfaces of the body against attack from micro organisms. Secretory IgA is synthesized locally by plasma cells and dimerized intracellularly with a cysteine-rich J-chain. Clone N1CLA was developed to produce reduced background staining that is associated with polyclonal antibodies on paraffin sections.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
N1CLA
Concentration:
Greater than or equal to 46 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Merluzzi S et al. Blood Journal. 2010; 115(14):2810-2817
References 2:
Fagarasan S and Honjo T. Current opinion in Immunology. 2004; 16(3):277-283
References 3:
Pilette C et al. European Respiratory Journal. 2001; 18:571-588
Polycomb-group proteins (PcG) such as EZH2 (Enhancer of Zeste Homolog 2 (Drosophila)) form multimeric gene repressing complexes involved in axial patterning, hematopoiesis and cell cycle regulation. PcG proteins ensure correct embryonic development by expressing homeobox genes as well as contributing to the regulation of lymphopoiesis.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
6A10
Concentration:
Greater than or equal to 20 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Van Kemenade FJ et al. Blood, 97(12): 38963901 (2001)
References 2:
Raaphorst Fm et al.American Journal of Pathology, 157(3): 709715 (2000)
References 3:
Kattan M. Journal of the National Cancer Institute. 95(9): 634635 (2003)
DOG-1, a 986 amino acid protein of unknown function, is expressed predominantly on the plasma membrane of gastrointestinal stromal tumors (GISTs) and is rarely expressed in other soft tissue tumors, which, due to appearance, can be confused with GISTs. Reactivity for DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express KIT antigen. The use of PBS-based diluents may result in increased background staining.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
K9
Concentration:
Greater than or equal to 12 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Novelli M et al. Histopathology. 2010; 57, 259-270
References 2:
Miettinen M et al. American Journal of Surgical Pathology, 2009; 33, 1401-1408
The CD30 antigen is a single chain glycoprotein with a molecular weight of 120 kD. CD30 antigen is known to act as a receptor for a cytokine ligand, CD30L, and may also play a role in the regulation of cellular growth and transformation. CD30 antigen is reported to be expressed on the surface of multinucleated Reed Sternberg cells, mononuclear Hodgkin's cells and in the majority of anaplastic large cell lymphomas. The CD30 antigen is expressed in non-Hodgkin's lymphoma and virally transformed cells, for example, EBV-transformed B cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
JCM182
Concentration:
Greater than or equal to 72 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kennedy M et al. Immunology. 2006; 118: 143-152
References 2:
Chiarle R et al. Clinical Immunology. 1999; 90(2):157-164
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. It is a single chain glycoprotein present on the surface of B lymphocytes and follicular dendritic cells of the hematopoietic system. CD19 is a crucial regulator in B cell development, activation and differentiation. On B cells, CD19 associates with CD21, CD81 and CD225 (Leu-13) forming a signal transduction complex. CD19 is expressed from the earliest recognizable B cell lineage stage, through development to B cell differentiation but is lost on maturation to plasma cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
BT51E
Concentration:
Greater than or equal to 35 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Fujimoto M and Sato S. Journal of Dermatological Science. 2007; 46:1-9
References 2:
Otero D et al. The Journal of Immunology. 2003; 170: 73-83
References 3:
Fujimoto M et al. Seminars in Immunology. 1998; 10:267-277
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. Originally named MN/G250, carbonic anhydrase IX (CAIX) is a cell surface transmembrane protein, which is predominantly found in the gastrointestinal tract and gallbladder. The glandular regions of normal colon are reported to be negative, but in the case of adenocarcinoma, the glands are positive. CAIX is also reported to be expressed in common epithelial tumors such as carcinomas of the esophagus, lung, colon, kidney, cervix and non-small cell lung carcinoma.In breast carcinomas, CAIX expression has been reported to be associated with malignant tissue. Expression of CAIX is reported to be absent in normal kidney, chromophobe carcinomas or oncocytomas; however, it is specifically expressed in clear cell renal carcinomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
TH22
Concentration:
Greater than or equal to 21 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Swietach P et al. Cancer and Metastasis Reviews. 2007; 26:299310
References 2:
Potter C and Harris A. Cell Cycle. 2004; 3(2):164167
Akt-1, also referred to as protein kinase B (PKB) or Rac alpha is a member of the Akt serin/threonine protein kinase family. It plays an important role in many biological responses including metabolism, cell survival and growth by phosphorylation and inactivating several targets including GSK 3 beta, caspase 9, BAD and the forkhead transcription factor. Akt-Phos is not recommended for use with PBS, since the use of PBS-based wash buffers and possibly PBS-based antibody diluents gives increased background staining and decreased staining intensity.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
LP18
Concentration:
Greater than or equal to 300 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Brazil D et al. Trends in Biochemical Sciences. 2004; 29(5):233242
References 2:
Nicholson K et al. Cellular Signalling. 2002; 14:381395
References 3:
Lawlor M et al. Journal of Cell Science. 2001; 114:29032910
ZAP-70 is a member of the syk family of proteins. It is expressed on T cells and NK cells and is required for the T cell receptor activation that triggers an immune response. CLL B cells that express the non-mutated immunoglobulin VH genes express levels of ZAP-70 protein that are comparable to those found in the blood T cells of healthy adults. Leukemic cells that express mutated Ig VH genes generally do not express detectable levels of ZAP-70 protein and this is correlated with the high level expression of CD38.
Antibody Isotype:
IgG2b, kappa
Monosan Range:
MONXtra
Clone:
L453R
Concentration:
Greater than or equal to 449 mg/L
Storage buffer:
PBS with sodium azide
Storage:
2-8°C
References 1:
Orchard J et al. Leukaemia and Lymphoma 2005; 46(12):16891698
References 2:
Wang J et al. Applied Immunohistochemistry and Molecular Morphology 2005; 13(4):323332
References 3:
Mustelin T and Tasken K.Biochemical Journal 2003; 371:1527
References 4:
Van Oers N and Weiss A. Seminars in Immunology 1995; 7:227236
Eukaryotic cells contain a number of types of cytoplasmic filamentous proteins, microtubule, microfilaments and intermediate-sized filaments (IF). Vimentin, a 57 kD protein that is an intermediate filament is reported to be expressed in most cells of mesenchymal origin, including fibroblasts, endothelial cells, smooth muscle, melanocytes as well as T and B lymphocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
SRL33
Concentration:
Greater than or equal to 60 mg/L
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Runembert I et al. Journal of Cell Science 115: 71324 (2002)
Thyroid peroxidase gene expression is under the regulation of thyroid stimulating hormone. In normal thyroid, expression of thyroid peroxidase (TPO) described immunohistochemically is reported to produce a diffuse, fine, granular cytoplasmic stain in all follicular cells. Some studies have shown qualitative, as well as quantitative differences in thyroid peroxidase expression in thyroid cancer compared to normal tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
AC25
Concentration:
Greater than or equal to 10.8 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kimura S et al. Proceedings of the National Academy of Science USA 84: 5555-5559 (1987)
References 2:
De Micco C et al. Cancer 67(12): 30363041 (1991)
References 3:
Czarnocka B et al. Breast Journal Cancer 85(6): 875880 (2001)
References 4:
Tanaka T et al. Journal of Pathology 179: 8994 (1996)
This monoclonal antibody recognizes both wild type and mutant forms of human p53 protein under denaturing and non-denaturing conditions. The epitope recognized by clone DO-7 can be destroyed by prolonged fixation in buffered formalin. The heat induced epitope retrieval technique may improve staining in some cases.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
DO-7
Concentration:
Greater than or equal to 22 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tiniakos DG et al. Cytopathology. 1996; 7(3): 178186
References 2:
Yoshida T et al. Journal of Pathology. 2003; 199(2):166175
References 3:
Burns ASYW et al. British Journal of Cancer. 2002; 86(7):11171123
References 4:
Tweddle DA et al. American Journal of Pathology. 2001; 158(6): 20672077
References 5:
Fernando SS et al. International Journal of Surgical Pathology. 2000; 8(3):213222
Epidermal growth factor receptor (EGFR) is a transmembrane protein receptor of 170 kD with tyrosine kinase activity. Increased levels of EGFR are reported to be linked with malignant transformation of squamous cells eg in squamous cell carcinoma of the lung, head, neck, skin, cervix and esophagus. EGFR may also play a role in the development and progression of hepatocellular carcinomas where recurrence rates are higher in EGFR-positive cases. This correlation has similarly been reported in colorectal cancers where EGFR, produced by tumor cells, plays an important role in the invasiveness and proliferation of colorectal cancers. The majority of published studies of EGFR expression in human breast cancer has similarly shown an association with EGFR expression where it is inversely related to estrogen receptor status.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
EGFR.113
Concentration:
Greater than or equal to 26 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Lodge AJ et al. Journal of Clinical Pathology. 2003; 56(4):300304
References 2:
Sriplakich S et al. BJU Int. 1999; 83(4):498503
References 3:
Inoue K et al. Acta Med Okayama 1998; 52(6):305310
References 4:
Tungekar MF and Linehan J. Journal of Clinical Pathology. 1998; 51:583587
Thyroglobulin is a heavily glycosylated protein of 670kD composed of two identical subunits and is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin provides iodination sites for the formation of the thyroid hormones.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
1D4
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Male DK et al. Immunology. 54: 419427 (1985)
References 2:
Shepherd PS et al. European Journal of Nuclear Medicine. 10: 291295 (1985)
References 3:
Chan CTJ et al. Clinical and Experimental Immunology. 70: 516523 (1987)
Mouse anti-Progesterone Receptor (A/B Forms), clone16 and SAN27
Antibody Type:
Monoclonal
Host Animal:
Mouse
Species Reactivity:
human
Immunogen:
Prokaryotic recombinant protein corresponding to the N-terminal region of the A form of the human progesterone receptor generating clone 16 and a prokaryotic recombinant protein corresponding to the 164 amino acid N-terminal region unique to the B form of the progesterone receptor generating clone SAN27.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. In vitro studies have indicated that PRA and PRB can activate different target genes and that PRA, in some circumstances, may act as a dominant inhibitor of the function of PRB and other steroid hormone receptors. PRA and PRB are both expressed in normal breast. Most endometrial carcinomas, however, are reported to express only one isoform with either PRA or PRB being expressed. The cocktail has been formulated using two clones, clone 16, specific for PRA, and SAN27, specific for PRB.
The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription factors. These two isoforms are transcribed from distinct estrogen receptor (ER)-inducible promoters within a single copy PR gene. Clone 16 is specific for a region of the N-terminus of the A form of PR. The precise epitope has not been mapped but it reacts with both A and B forms of PR by Western blot but only with the A form by immunohistochemistry. This suggests that the epitope is inaccessible in the native folded B form of the protein.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
16
Concentration:
Greater than or equal to 324 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Hungermann D et al. Journal of Pathology 2002; 198: 487494
Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. The introduction of highly specific monoclonal antibodies to ER has allowed the determination of receptor status of breast tumors to be carried out in routine histopathology laboratories.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
6F11
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Bevitt DJ et al. Journal of Pathology 1997; 183(2), 228232
References 2:
Kaplan, PA et al. Am J Clin Pathol 2005: 276280
References 3:
Zafrani B et al. Histopathology 2000; 37(6), 536545
References 4:
Harvey JM et al. Journal of Clinical Oncology 1999; 17(5), 14741481
References 5:
Khan SA et al.European Journal of Cancer 2000; 36(Suppl 4), S27S28
Analyte Specific Reagent. Analytical and performance characteristics are not established. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
Ham3/17B2
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
Analyte Specific Reagent. Analytical and performance characteristics are not established. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
Ham1/7B6
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
NCL-b-DG is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
43DAG1/8D5
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 2:
Muschen M et al. Cancer Research. 61 (5): 20802084 (2001)
References 3:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
Synaptophysin is an integral membrane glycoprotein with a molecular weight of 38 kD. It is reported to occur in presynaptic vesicles of neurons in brain, spinal cord, retina, in similar vesicles of the adrenal medulla as well as in neuromuscular junctions. Synaptophysin may be involved in synaptic vesicle formation and exocytosis. Synaptophysin is reported to be expressed in a wide spectrum of neuroendocrine tumors including neuroblastomas, ganglioneuroblastomas, phaeochromocytomas, chromaffin and non-chromaffin paragangliomas. Synaptophysin is also reported to be expressed in neuroendocrine tumors of epithelial type including pituitary adenomas, islet cell tumors, medullary carcinomas of thyroid, parathyroid adenomas, carcinoids of the bronchopulmonary and gastrointestinal tracts, neuroendocrine carcinomas of the bronchopulmonary and gastrointestinal tract and neuronendocrine carcinomas of the skin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
27G12
Concentration:
Greater than or equal to 42 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Takeda S et al. Neuropathology. 2003; 23(4):254261
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
