DOG-1, a 986 amino acid protein of unknown function, is expressed predominantly on the plasma membrane of gastrointestinal stromal tumors (GISTs) and is rarely expressed in other soft tissue tumors, which, due to appearance, can be confused with GISTs. Reactivity for DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express KIT antigen. The use of PBS-based diluents may result in increased background staining.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
K9
Concentration:
Greater than or equal to 12 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Novelli M et al. Histopathology. 2010; 57, 259-270
References 2:
Miettinen M et al. American Journal of Surgical Pathology, 2009; 33, 1401-1408
The CD30 antigen is a single chain glycoprotein with a molecular weight of 120 kD. CD30 antigen is known to act as a receptor for a cytokine ligand, CD30L, and may also play a role in the regulation of cellular growth and transformation. CD30 antigen is reported to be expressed on the surface of multinucleated Reed Sternberg cells, mononuclear Hodgkin's cells and in the majority of anaplastic large cell lymphomas. The CD30 antigen is expressed in non-Hodgkin's lymphoma and virally transformed cells, for example, EBV-transformed B cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
JCM182
Concentration:
Greater than or equal to 72 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kennedy M et al. Immunology. 2006; 118: 143-152
References 2:
Chiarle R et al. Clinical Immunology. 1999; 90(2):157-164
CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. It is a single chain glycoprotein present on the surface of B lymphocytes and follicular dendritic cells of the hematopoietic system. CD19 is a crucial regulator in B cell development, activation and differentiation. On B cells, CD19 associates with CD21, CD81 and CD225 (Leu-13) forming a signal transduction complex. CD19 is expressed from the earliest recognizable B cell lineage stage, through development to B cell differentiation but is lost on maturation to plasma cells.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
BT51E
Concentration:
Greater than or equal to 35 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Fujimoto M and Sato S. Journal of Dermatological Science. 2007; 46:1-9
References 2:
Otero D et al. The Journal of Immunology. 2003; 170: 73-83
References 3:
Fujimoto M et al. Seminars in Immunology. 1998; 10:267-277
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. Originally named MN/G250, carbonic anhydrase IX (CAIX) is a cell surface transmembrane protein, which is predominantly found in the gastrointestinal tract and gallbladder. The glandular regions of normal colon are reported to be negative, but in the case of adenocarcinoma, the glands are positive. CAIX is also reported to be expressed in common epithelial tumors such as carcinomas of the esophagus, lung, colon, kidney, cervix and non-small cell lung carcinoma.In breast carcinomas, CAIX expression has been reported to be associated with malignant tissue. Expression of CAIX is reported to be absent in normal kidney, chromophobe carcinomas or oncocytomas; however, it is specifically expressed in clear cell renal carcinomas.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
TH22
Concentration:
Greater than or equal to 21 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Swietach P et al. Cancer and Metastasis Reviews. 2007; 26:299310
References 2:
Potter C and Harris A. Cell Cycle. 2004; 3(2):164167
Akt-1, also referred to as protein kinase B (PKB) or Rac alpha is a member of the Akt serin/threonine protein kinase family. It plays an important role in many biological responses including metabolism, cell survival and growth by phosphorylation and inactivating several targets including GSK 3 beta, caspase 9, BAD and the forkhead transcription factor. Akt-Phos is not recommended for use with PBS, since the use of PBS-based wash buffers and possibly PBS-based antibody diluents gives increased background staining and decreased staining intensity.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
LP18
Concentration:
Greater than or equal to 300 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Brazil D et al. Trends in Biochemical Sciences. 2004; 29(5):233242
References 2:
Nicholson K et al. Cellular Signalling. 2002; 14:381395
References 3:
Lawlor M et al. Journal of Cell Science. 2001; 114:29032910
ZAP-70 is a member of the syk family of proteins. It is expressed on T cells and NK cells and is required for the T cell receptor activation that triggers an immune response. CLL B cells that express the non-mutated immunoglobulin VH genes express levels of ZAP-70 protein that are comparable to those found in the blood T cells of healthy adults. Leukemic cells that express mutated Ig VH genes generally do not express detectable levels of ZAP-70 protein and this is correlated with the high level expression of CD38.
Antibody Isotype:
IgG2b, kappa
Monosan Range:
MONXtra
Clone:
L453R
Concentration:
Greater than or equal to 449 mg/L
Storage buffer:
PBS with sodium azide
Storage:
2-8°C
References 1:
Orchard J et al. Leukaemia and Lymphoma 2005; 46(12):16891698
References 2:
Wang J et al. Applied Immunohistochemistry and Molecular Morphology 2005; 13(4):323332
References 3:
Mustelin T and Tasken K.Biochemical Journal 2003; 371:1527
References 4:
Van Oers N and Weiss A. Seminars in Immunology 1995; 7:227236
Eukaryotic cells contain a number of types of cytoplasmic filamentous proteins, microtubule, microfilaments and intermediate-sized filaments (IF). Vimentin, a 57 kD protein that is an intermediate filament is reported to be expressed in most cells of mesenchymal origin, including fibroblasts, endothelial cells, smooth muscle, melanocytes as well as T and B lymphocytes.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
SRL33
Concentration:
Greater than or equal to 60 mg/L
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Runembert I et al. Journal of Cell Science 115: 71324 (2002)
Thyroid peroxidase gene expression is under the regulation of thyroid stimulating hormone. In normal thyroid, expression of thyroid peroxidase (TPO) described immunohistochemically is reported to produce a diffuse, fine, granular cytoplasmic stain in all follicular cells. Some studies have shown qualitative, as well as quantitative differences in thyroid peroxidase expression in thyroid cancer compared to normal tissue.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
AC25
Concentration:
Greater than or equal to 10.8 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Kimura S et al. Proceedings of the National Academy of Science USA 84: 5555-5559 (1987)
References 2:
De Micco C et al. Cancer 67(12): 30363041 (1991)
References 3:
Czarnocka B et al. Breast Journal Cancer 85(6): 875880 (2001)
References 4:
Tanaka T et al. Journal of Pathology 179: 8994 (1996)
Oct-3/4 is a member of the POU homeodomain family of transcription factors, which is expressed by embryonic stem cells and germ cells. A critical amount of Oct-3/4 is required to maintain stem cell self replication. Down regulation of Oct-3/4 levels are associated with loss of pluripotency. Oct-3/4 has been proposed as a useful marker for germ cell tumors which exhibit features of pluripotentiality, including seminoma/dysgerminoma/germinoma and embryonal carcinoma, and establishing a germ cell origin for some metastatic tumors of uncertain primary origin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
N1NK
Concentration:
Greater than or equal to 69 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Del Sordo R et al. Histology and histopathology. 2014; 29(1):101-106
References 2:
Miettinen M et al. American Journal of Surgical Pathology. 2014; 38(3):410-420
References 3:
Paine SML et al. Neuropathology and applied neurobiology. 2014; 40:544-550
References 4:
Antic T et al. American Journal of Pathology. 2011; 136:872-880
Napsin A has a specific function in normal alveolar epithelium and is proposed to play a role in the proteolytic processing of surfactant precursors. Napsin A is reported to be predominantly expressed in lamellar bodies of type II pneumocytes, secondary lysosomes of alveolar macrophages, respiratory epithelium of terminal and respiratory bronchioles, plasma cells, within a subset of lymphocytes in normal lung, as well as in epithelial cells of renal tubules in normal kidney and is weakly expressed in normal spleen. Studies have reported that Napsin A is expressed in 90% of primary lung adenocarcinomas.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
IP64
Concentration:
Greater than or equal to 8.3 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Yamashita Y et al. Modern Pathology. 2015; 28: 111-11
References 2:
Kandalaft PL et al. American Journal of Clinical Pathology. 2014; 142: 830-836
Mismatch repair gene MutS Homolog 6 is a ubiquitous gene encoding the mismatch repair protein (MMR) MutS protein homolog 6 (MSH6). MSH6 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG
Monosan Range:
MONXtra
Clone:
PU29
Concentration:
Greater than or equal to 200 mg/L
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Warren J et al. Molecular Cell. 2007; 26:579-592
References 2:
Marti T et al. Journal of Cellular Physiology. 2002; 191:28-41.
Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) MutL protein homolog 1 (MLH1). MLH1 functions by repairing mutations occurring during DNA replication, in normal proliferating cells.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
ES05
Concentration:
Greater than or equal to 165 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Tamura G et al.World Journal of Gastroenterology 2006; 12(2): 192198
References 2:
Abdel-Rahman W et al. Critical Reviews in Oncology/Hematology 2006; 58: 208220
References 3:
Mitchell R et al. American Journal of Epidemiology 2002; 156:885902
References 4:
Kuismanen S et al. American Journal of Pathology 2000; 156(5): 17731779
CD33 antigen is reported to appear on myelomonocytic precursor cells after CD34 antigen expression. It then continues to be expressed on both the myeloid and monocyte lineages, although it is reported to be absent on granulocytes. It has been reported that expression of CD33 is restricted to monocytes, premyelocytes, myeloid blasts, some acute undifferentiated leukemias and acute lymphoblastic leukemias.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
PWS44
Concentration:
Greater than or equal to 417 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Bradshaw EM et al. Nature Neuroscience. 2013, 16(7): 848 852
References 2:
Hoyer JD et al. American Journal of Clinical Pathology. 2008; 129(2): 316 323.
The CD7 molecule is a membrane-bound glycoprotein of 40 kD and is the earliest T cell specific antigen to be expressed in lymphocytes. CD7 antigen is also the only early marker to persist throughout differentiation. The function and role of the CD7 molecule has not yet been fully identified, although the activation of T cells with gamma/delta receptors has been proposed based on mAb-induced activation. CD7 antigen is reported to be found on the majority of peripheral blood T cells, most natural killer cells and thymocytes.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
LP15
Concentration:
Greater than or equal to 351 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Leong FJW et al. The Journal of Histotechnology. 2002; 25(4):215-227
References 2:
Ormsby A et al. Journal of the American Academy of Dermatology. 2001; 45(3):405-413
Geminin is a protein of 209 amino acids thought to be involved in the control of DNA replication via the interaction with Cdt1. Geminin is not found in the G1 phase of the cell cycle, but is first expressed in the G1 to S transition phase, with expression levels rising through the rest of the cell cycle and levels reaching a maximum during mitosis. It has been proposed that geminin may be a tumor suppressor protein. Geminin is reported to be expressed in proliferating lymphocytes and epithelial cells, for example, germinal centers in tonsil as well as in colon, spermatocytes, seminiferous tubules of the testes, within the basal layers of the squamous epithelium of the skin and breast. Geminin is reported to be upregulated in cancers such as non-Hodgkin's lymphoma, B cell lymphoma, breast carcinoma and colon carcinoma.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
EM6
Concentration:
Greater than or equal to 19 mg/L
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
McGarry TJ and Kirschner MW. Cell. 1998; 93:10431053
Prokaryotic recombinant protein corresponding to a 70 amino acid component of the N-terminal region of the cytokeratin 20 intermediate filament protein
Cytokeratin 20 has been demonstrated to be almost entirely confined to the gastric and intestinal epithelium, urothelium and Merkel cells of the skin. Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest due to its restricted tissue expression. In normal tissue, cytokeratin 20 is expressed in intestinal epithelium, gastric foveolar epithelium, a number of endocrine cells in the upper portions of the pyloric glands, urothelium and Merkel cells in epidermis. In tumors it is reported, there is a marked difference in the expression of cytokeratin 20 within different carcinomas. Neoplasms expressing cytokeratin 20 are derived from normal epithelia which themselves expressed cytokeratin 20. Colorectal carcinomas consistently express cytokeratin 20, while gastric adenocarcinomas express cytokeratin 20 to a lesser degree. Adenocarcinomas of the gall bladder and bile duct, ductal cell adenocarcinomas of the pancreas, mucinous ovarian tumors, Merkel cell tumors and transitional cell carcinomas have also been reported to express cytokeratin 20.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
PW31
Concentration:
Greater than or equal to 37 mg/L
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
Storage:
2-8°C
References 1:
Campbell F and Herrington CS. Current Diagnostic Pathology. 2001; 7:113-122
References 2:
Leech SN et al. Journal of Clinical Pathology. 2001; 54:727-729
References 3:
Ferrari L et al. Anticancer Research. 1999; 19: 3415-3428
Calretinin is a calcium-binding protein of 29 kD that is a member of the family of so-called EF-hand proteins that also includes S-100 proteins. Calretinin is reported to be abundantly expressed in neurons. Outside the nervous system, calretinin is reported to be expressed in a range of cell types including mesothelial cells, steroid producing cells, (for example adrenal cortical cells, Leydig cells, ovarian theca interna cells, Sertoli cells, some neuroendocrine cells, eccrine sweat glands) and other cell types.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
CAL6
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Groves P et al. Acta Biochimica Polonica 2001, 48(1), 113-119
References 2:
Rogers JH .The Journal of Cell Biology 1987, 105, 1343-1353
Bcl-6 is a proto-oncogene that encodes a Kruppel-type zinc-finger protein of 95 kD and shares homology with other transcription factors. Bcl-6 protein is mainly expressed in normal germinal center B cells and related lymphomas. It has been shown that the Bcl-6 proto-oncogene is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have also been detected in 33-45% of diffuse large B cell lymphomas. Immunohistochemistry has been reported to show the Bcl-6 gene product to be detectable in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.
The CD3 molecule consists of five different polypeptide chains with molecular weights ranging from 16 to 28 kD. The CD3 antigen is first detected in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. Clone LN10 is specific for the non-glycosylated epsilon chain of the human CD3 molecule. Clone LN10 recognizes T cells in thymus, bone marrow, peripheral lymphoid tissue and blood and is a pan T cell marker.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
LN10
Concentration:
Greater than or equal to 32 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Krynitz B et al. Acta Dermato Venerologica. 2010; 90:379-385
Wilms' tumor protein (WT1) has a role in transcriptional regulation and is expressed in the kidney and a subset of hematopoietic cells. Alteration of transcription factor function is a common mechanism in oncogenesis. The WT1 protein contains a DNA binding domain and any deletions or point mutations of the WT1 gene which destroy this activity result in the development of the childhood nephroblastoma Wilms' tumor and Denys-Drash syndrome. The description of WT1 involvement in nephroblastoma is not clear.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
WT49
Concentration:
Greater than or equal to 44 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Omeroglu A and Omeroglu G. Arch Pathol Lab Med. 2003; 127:e347-e348
References 2:
Lee SB and Haber DA. Experimental Cell Research. 2001; 264:74-99
IL-6 is a multifunctional cytokine that is secreted by both lymphoid and non-lymphoid cells. It plays a key role in immune responses, hematopoiesis and is an important cytokine in cell proliferation and differentiation. It may also play an important role as an autocrine growth factor in metastatic prostate cancer. IL-6 has been reported to play a role in secretion or release of pituitary hormone in pituitary hormone secreting cells and adenomas. In addition, IL-6 has been suggested to have a trophic effect in nerve cells and to have a direct pathogenic role in CNS disorders. There are an increasing number of reports that cytokines of the IL-6 family play an important regulatory role in heart physiology.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
10C12
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Salgado R et al. International Journal of Cancer. 103 (5): 642646 (2003)
References 2:
Kurotani R et al. Modern Pathology. 14 (8): 791797 (2001)
References 3:
Menet E et al. European Cytokine Network. 12 (4): 639646 (2001)
References 4:
Ono S et al. Journal of the Neurological Sciences. 187 (12): 2734 (2001)
References 5:
Yasukawa K et al. The EMBO Journal. 6 (10): 29392945 (1987)
Cytokeratins are intermediate filament proteins present in epithelial cells. They are expressed in a tissue-specific manner in normal organs and the tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type B subfamily of cytokeratins. Its distribution is confined to glandular and transitional epithelia. Cytokeratin 7 is reported to be expressed in abundance in cultured bronchial and mesothelial cells but only at lower levels in cultured epidermal cells. The predicted amino acid sequence of this keratin has revealed a striking difference between this keratin and the type II keratins expressed in epidermal cells. Cytokeratin 7 has been reported in adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as in carcinomas of the bladder and chromophobe renal cell carcinoma. Cytokeratin 7 and Cytokeratin 20 expression have been reported to show characteristic patterns on primary and metastatic lung and colorectal adenocarcinomas.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
RN7
Concentration:
Greater than or equal to 17 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
van de Molengraft FJJM et al.Histopathology. 1993; 22:35-38
References 2:
van Niekerk CC et al. Journal of Pathology. 1991; 165(2):145-15
CD79b, also known as B29 and Ig-beta is thought to function in the cellular activation and signaling that occurs when surface immunoglobulin (Ig) on B cells binds antigen or becomes cross-linked by anti-Ig antibody. This function occurs with the formation of a membrane signaling complex that is associated with Ig at the surface of B cells. CD79b, together with CD79a, forms the B cell antigen receptor (mlg) complex.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
JS01
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Matutes E. Journal of Clinical Pathology. 55: 180183 (2002)
References 2:
McCarron K F et al. American Journal of Clinical Pathology. 113 (6): 805813 (2000)
References 3:
Moreau E J et al. American Journal of Clinical Pathology. 108: 378382 (1997)
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