S-100A and S-100B proteins are two members of the S-100 family of proteins. S-100A subtypes are composed of one alpha and one beta chain, whereas S-100B is composed of two beta chains. S-100 protein is reported to be expressed in neuroectodermal tissue, including nerves and melanocytes. Langerhans cells in skin are also reported to express S-100 protein. It is noteworthy that S-100 protein is highly soluble and may be eluted from frozen tissue during immunohistochemical procedures. Clone EP32 was raised against S-100 beta protein. Immunoreactivity was observed in neuroectodermal tissue e.g. melanocytes, nerve fibres, dendritic cells, adipocytes and a percentage of macrophages, lymphocytes and plasma cells.
Antibody Isotype:
IgG
Monosan Range:
MONXtra
Clone:
EP32
Concentration:
Greater than or equal to 30 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Chen H et al. American Journal of Cancer Research 2014;4(2):89-115
References 2:
Torlakovic EE et al.Applied Immunohistochemistry & Molecular Morphology. 2015; 23(1):1-18
Eukaryotic cells contain a number of types of cytoplasmic filamentous proteins, microtubule, microfilaments and intermediate-sized filaments (IF). Vimentin, a 57 kD protein that is an intermediate filament is reported to be expressed in most cells of mesenchymal origin, including fibroblasts, endothelial cells, smooth muscle, melanocytes as well as T and B lymphocytes.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
V9
Concentration:
Greater than or equal to 16 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Colella R et al. American Journal of Surgical Pathology. 2010;34:10-17
References 2:
Gimelli S et al. Molecular Cancer. 2009;8:52
References 3:
Arnardottir S et al. Neurosurgery and Psychiatry. 2004;75(6):917-920
References 4:
Vargel I et al. American Journal of Medical Genetics. 2002; 109(1):22-35
References 5:
Osborn M et al. European Journal of Cell Biology. 1984; 34:137-143
Alpha-synuclein is a protein of 140 amino acids and a member of the synuclein family. It shares 61% sequence homology with beta-synuclein and is highly conserved between vertebrate species. It does not possess a signal sequence suggesting that it is an intracellular protein. All synucleins have an unusual organization based around the eleven residue repeating motif and an alpha-helical secondary structure resembling those found in the lipid-binding domain of exchangeable apolipoproteins, including Apo E. This homology suggests a direct interaction of alpha-synuclein with membranes consistent with its affinity for synaptosomes.Clone KM51 is specific for alpha-synuclein and is unreactive with beta-synuclein. Pretreatment of tissue sections with 98-100% formic acid is also recommended.
Antibody Isotype:
IgG1, kappa
Monosan Range:
MONXtra
Clone:
KM51
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Baba M et al. American Journal of Pathology. 152 (4): 879884 (1998)
References 2:
Polymeropoulos MH et al. Science. 276: 20452047 (1997)
References 3:
Spillantini MG et al. Nature. 388: 839840 (1997)
References 4:
Weinreb PH et al. Biochemistry. 35 (43): 1370913715 (1996)
The D-type cyclins are a family of proteins which function primarily by regulating the activity of cyclin dependent kinases in the G1 phase of the cell cycle. Cyclin D1, a protein of 36 kD, is also known as PRAD1 or bcl-1. Maximum expression of cyclin D1 occurs at a critical point in mid to late G1 phase of the cell cycle. The cyclin D1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphomas due to the chromosomal translocation t(11;18).
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
P2D11F11
Concentration:
Greater than or equal to 19 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
McIntosh GG et al. Oncogene. 1995; 11:885891
References 2:
Saiz AD et al. Journal of Pathology. 2002; 198(2):157162
References 3:
Mommers ECM et al. Journal of Pathology. 2001; 194(3):327333
References 4:
Saito T et al. Journal of Pathology. 2001; 195(2):222228
References 5:
Sheyn I et al. Human Pathology. 1997; 28(3):270276
This antibody reacts with full-size calpain 3 (94 kD) plus an additional fragment at 30 kD in human skeletal muscle. A band of 94 kD is seen with rabbit and dog muscle while extracts of hamster muscle show reactivity with the 94 kD band and a larger species of approximately 110 kD. This 110 kD band is the principal immunoreactive species seen in rat muscle extracts. This antibody produces no bands with mouse, pig or chicken muscle. Reconstitute with the volume of sterile distilled water indicated on the vial label.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
Calp3d/2C4
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Anderson LVB et al. American Journal of Pathology. 153 (4): 11691179 (1998)
References 2:
Topaloglu H et al. Neuropediatrics. 28: 212216 (1997)
Analyte Specific Reagent. Analytical and performance characteristics are not established. NCL-EMERIN is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
4G5
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Muschen M et al. Cancer Research. 61 (5): 20802084 (2001)
References 2:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
NCL-b-DG is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
43DAG1/8D5
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 2:
Muschen M et al. Cancer Research. 61 (5): 20802084 (2001)
References 3:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
The muscle-specific form of laminin, merosin, is composed of three chains: alpha 2, beta 1 and gamma 1.Analyte Specific Reagent. Analytical and performance characteristics are not established. Reacts strongly with laminin alpha 2 chain of merosin in human and rabbit skeletal muscle. No reaction is observed in muscle sections from mouse, rat, dog, chicken, hamster or pig. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
Mer3/22B2
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
This antibody reacts with full-size calpain 3 (94 kD) plus an additional breakdown product at 60 kD in human skeletal muscle. The 94 kD band can be seen in muscle extracts from rabbit, mouse, dog, chicken, hamster, pig and rat. Degraded calpain 3 bands starting at approximately 60 kD are also usually present. Additional bands corresponding in size to calpains 1 and/or 2 can be detected in skeletal muscle from mouse, rat, chicken and hamster. Reconstitute with the volume of sterile distilled water indicated on the vial label.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
Calp3c/12A2
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Anderson LVB et al. American Journal of Pathology. 153 (4): 11691179 (1998)
References 2:
Topaloglu H et al. Neuropediatrics. 28: 212216 (1997)
In normal tissues cytokeratin 17 is reported to be expressed in basal cells of complex epithelia, for example, basal cells of pseudostratified epithelium in the trachea, larynx, bronchi, myoepithelial cells in salivary glands and sweat glands. In neoplastic tissue, cytokeratin 17 is reported to be expressed in squamous cell carcinomas of the lung, cervix and oral cavity. CK17 reacts with the human cytokeratin intermediate filament protein (46 kD) identified as cytokeratin 17.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
E3
Concentration:
Greater than or equal to 8 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Ansai S et al. Journal of Dermatology. 2011; 38(10):951-958
The CD68 molecule is a 110 kD intracellular glycoprotein primarily reported to be associated with cytoplasmic granules and to a lesser extent the membranes of macrophages. Markers to CD68 antigen are the most frequently used for the identification of macrophages in immunohistochemistry; however, CD68 is also found in monocytes, neutrophils, basophils and large lymphocytes. The function of the CD68 molecule is not certain but these lysosomal membrane proteins are major components and may protect the membranes from attack by acid hydrolases. It is unclear if the surface-associated CD68 protein is functionally significant or due to leakage from the lysosomes. CD68 protein expression has been demonstrated in stimulated T cells and NK cells and non-hematopoietic tissues such as liver and renal tubules.
Antibody Isotype:
IgG2a, kappa
Monosan Range:
MONXtra
Clone:
514H12
Concentration:
Greater than or equal to 37 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Gu M et al. Annals of Diagnostic Pathology. 2007; 11:64-67
References 2:
Da Costa CET et al. The Journal of Experimental Medicine. 2005; 201(5):687-693
The CD38 molecule is a type II single transmembrane glycoprotein with a molecular weight of 46 kD. It is an ectoenzyme with the activities of ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD glycohydrolase and is involved in both the formation and hydrolysis of cADPR, a second messenger that regulates the mobilization of intracellular Ca2+ ions. Although the CD38 molecule was originally identified as a T lymphocyte differentiation antigen, it is reported to be expressed in a wide range of cells and tissues. CD38 antigen can deliver potent growth and differentiation signals to lymphoid and myeloid cells. It is found on immature cells of the B and T cell lineages but not on most mature resting peripheral lymphocytes. It is also present on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, Ig-secreting plasma cells, monocytes, NK cells, erythroid and myeloid progenitors in the bone marrow and brain cells. CD38 antigen has also been reported in neurofibrillary tangles, the pathological indicator of Alzheimer's disease that occurs in the neuronal perikarya and proximal dendrites.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
SPC32
Concentration:
Greater than or equal to 46 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Krukemeyer MG et al. Transplantationsmedizin. 2003; 15:4046
The CD79 complex is a disulfide-linked heterodimer which is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of polypeptides and immunoglobulin constitute the B cell antigen receptor. The two components of this complex are designated CD79a and CD79b. The CD79a antigen is reported to first appear at the pre-B cell stage, early in maturation, and persist until the plasma cell stage where it is found as an intracellular component. It is not present in myeloid or T cell lines.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
JCB117
Concentration:
Greater than or equal to 20 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Bhargava P et al. American Journal of Clinical Pathology. 2007; 128(2): 306-313
References 2:
Torlakovic E et al. The American Journal of Surgical Pathology. 2002; 26(10): 1343-1350
References 3:
Blakolmer K et al. Modern Pathology. 2000; 13(7): 766-772
References 4:
Pilozzi E et al. The Journal of Pathology. 1998;186(2):140-143
Cytokeratins 14 and 5 are useful to distinguish stratified epithelial cell types from simple epithelial cell types. Cytokeratin 14 has been reported to be expressed in neoplasms of squamous cell origin.Clone LL002 reacts with the human cytokeratin intermediate filament protein (50 kD) identified as cytokeratin 14.
Antibody Isotype:
IgG3
Monosan Range:
MONXtra
Clone:
LL002
Concentration:
Greater than or equal to 24 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Reis-Filho JS et al. Journal of Clinical Pathology. 2004; 57:83-86
References 2:
Sivard P et al. Experimental Dermatology. 2003; 12(4):346355
References 3:
Fong LYY et al. Cancer Research. 2003; 63:186195
References 4:
Nagao T et al. Histopathology. 2001; 38(1):3036
References 5:
Nakayama H et al. Japanese Journal of Clinical Oncology. 1997; 27(6):427432
aveolin-1 is a major structural component of caveolae, which are vesicular invaginations present on the plasma membrane of different cell types. It plays a regulatory role in several signaling pathways and is reported to be most abundantly expressed in terminally differentiated mesenchymal cells such as smooth muscle cells, adipocytes and endothelial cells. High levels are also reported in fibroblasts where a fine granular membranous and diffuse cytoplasmic staining pattern is described.
Antibody Isotype:
IgG2a, kappa
Monosan Range:
MONXtra
Clone:
4D6
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15mM sodium azide
Storage:
2-8°C
References 1:
Wiechen K et al. American Journal of Pathology. 158 (3): 833839 (2001).
References 2:
Scherer PE et al. PNAS. 93: 131135 (1996)
References 3:
Lisanti MP et al. Journal of Cellular Biology. 126 (1): 111126 (1994).
Analyte Specific Reagent. Analytical and performance characteristics are not established. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
Ham1/7B6
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
Analyte Specific Reagent. Analytical and performance characteristics are not established. The product is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
Ham3/17B2
Concentration:
n/a
Storage buffer:
Lyophilized
Storage:
2-8°C
References 1:
Marafioti T et al. American Journal of Pathology. 162 (3): 861871 (2003
References 2:
Hess J et al. Molecular and Cellular Biology. 21 (5): 15311539 (2001)
References 3:
Re D et al. Cancer Research. 61 (5): 20802084 (2001)
References 4:
Luo Y and Roeder R G. Molecular and Cellular Biology. 15 (8): 41154124 (1995)
Oct-3/4 is a member of the POU homeodomain family of transcription factors, which is expressed by embryonic stem cells and germ cells. A critical amount of Oct-3/4 is required to maintain stem cell self replication. Down regulation of Oct-3/4 levels are associated with loss of pluripotency. Oct-3/4 has been proposed as a useful marker for germ cell tumors which exhibit features of pluripotentiality, including seminoma/dysgerminoma/germinoma and embryonal carcinoma, and establishing a germ cell origin for some metastatic tumors of uncertain primary origin.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
N1NK
Concentration:
Greater than or equal to 69 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Del Sordo R et al. Histology and histopathology. 2014; 29(1):101-106
References 2:
Miettinen M et al. American Journal of Surgical Pathology. 2014; 38(3):410-420
References 3:
Paine SML et al. Neuropathology and applied neurobiology. 2014; 40:544-550
References 4:
Antic T et al. American Journal of Pathology. 2011; 136:872-880
Granzymes are neutral serine proteases which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. These CTL and NK cells are heavily involved in the elimination of neoplastic and virally infected cells. Secretory granules containing perforin and granzymes are instrumental in undertaking cytolytic activity. Granzyme B is understood to enter a target cell through a perforin pore-formed channel to induce DNA fragmentation and apoptosis. Granzyme B has also been described in neoplastic CTL and NK cells.
Antibody Isotype:
IgG2a
Monosan Range:
MONXtra
Clone:
11F1
Concentration:
Greater than or equal to 47 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Lee HK et al. J Korean Med Sci. 2003; 18:272276
References 2:
Theate I et al.European Journal of Haematology. 2002; 69(4):248253
The neural cell adhesion molecules are a family of closely-related cell surface glycoproteins thought to play a role in embryogenesis, development and contact-mediated interactions between neural cells. The CD56 antigen (NCAM) consists of four major isoforms generated by differential splicing of the RNA transcript from a single gene located on chromosome 5. The CD56 antigen is expressed on neurons, astrocytes, Schwann cells, NK cells and a subset of activated T lymphocytes.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
CD564
Concentration:
Greater than or equal to 11 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Nakamoto Y et al. Clinical and Experimental Immunology. 2007; 147:296-305
References 2:
Wicherek L et al. Reproductive Biology and Endocrinology. 2006; 4:41
References 3:
Wicherek l et al. Journal of Reproductive Immunology. 2006; 70:119-131
The CD71 molecule is a type II membrane glycoprotein with a molecular weight of approximately 180 kD. It is known as the transferrin receptor and is composed of two disulfide-bonded 90 kD subunits. The CD71 molecule plays a critical role in cell proliferation by controlling the supply of iron, an essential component for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. CD71 protein is reported to be expressed on activated B and T cells, macrophages, proliferating cells and metabolically active cells, for example, neurons.
Antibody Isotype:
IgG2b
Monosan Range:
MONXtra
Clone:
10F11
Concentration:
Greater than or equal to 42 mg/L
Storage buffer:
Tissue culture supernatant with sodium azide
Storage:
2-8°C
References 1:
Dong HY et al. American Journal of Surgical Pathology. 2011; 35(5):723-732
Polycomb-group proteins (PcG) such as EZH2 (Enhancer of Zeste Homolog 2 (Drosophila)) form multimeric gene repressing complexes involved in axial patterning, hematopoiesis and cell cycle regulation. PcG proteins ensure correct embryonic development by expressing homeobox genes as well as contributing to the regulation of lymphopoiesis.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
6A10
Concentration:
Greater than or equal to 20 mg/L
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Van Kemenade FJ et al. Blood, 97(12): 38963901 (2001)
References 2:
Raaphorst Fm et al.American Journal of Pathology, 157(3): 709715 (2000)
References 3:
Kattan M. Journal of the National Cancer Institute. 95(9): 634635 (2003)
Folate is a basic component of cell metabolism and DNA synthesis and repair. It is involved in essential one-carbon transfer reactions and is a vitamin required by both normal and tumor cells. Folate entry into cells is facilitated via two different systems: the reduced folate carrier, which utilizes a bidirectional anion-exchange mechanism, and the folate receptor system. Folate receptor alpha is a membrane-bound member of the folate receptor family, facilitating folate transport via a mechanism termed potocytosis where the receptor is internalized and then recycled back to the cell membrane. Folate receptor alpha expression is reported to be highly restricted in normal tissues and only selectively overexpressed in a limited number of epithelial malignancies.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
BN3.2
Concentration:
Greater than or equal to 67 mg/L
Storage buffer:
Tissue culture supernatant with 15mM Sodium azide
Storage:
2-8°C
References 1:
Smith AE et al. Hybridoma. 2007; 26(5):281288
References 2:
Kelemen L. International Journal of Cancer. 2006; 119:243250
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
