ZTL Zeitlupe (Adagio Protein 1) is a component of an E3 ubiquitin ligase complex which is playing a central role in blue light-dependent circadian cycles. Alternative names: Adagio protein 1 (ADO1), F-box only protein 2b (FBX2b), Flavin-binding kelch repeat F-box protein 1-like protein 2, FKF1-like protein 2 (FKL2), LOV kelch protein 1 (LKP1), Clock-associated PAS protein ZTL, ZEITLUPE (ZTL).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Hordeum vulgareSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ZTL1 protein sequence, UniProt: Q94BT6, TAIR: AT5G57360
Zearalenone is a RAL and F-2 mycotoxin produced by some Fusarium and Gibberella species. It is a potent estrogenic metabolite and is the primary toxin causing infertility, abortion or other breeding problems, especially in swine. Zearalenone is found in a many cereal crops, such as maize, barley, oats, wheat, rice, sorghum as well as in bread all over the world.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Lyophilized powder is stable for a minimum of 2 years at -20 °C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°Cfor up to a month and make aliquots for extended storage.
Host Animal:
Mouse
Species Reactivity:
Antibody detects zearalenone mycotoxin of Fusarium sp.
COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Emiliania huxleyi Species of your interest not listed? Contact us
Immunogen:
Recombinant ZCP2 protein of Chlamydomonas reinhardtii, ID 536252, UniProt: A0A059VIF5
This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 | 100 kDa (probably due to glycosylation)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
ZCP1 belongs to a large family of putative metallochaperones, likned with maintainance of zinc homeostasis in all living organisms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Emiliania huxleyi Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C, Store in small aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Native and denatured forms of GFP and Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP)
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of YFP protein from the jellyfish Aequorea victoria, UniProt:A0A059PIR9
Applications:
ELISA (ELISA), Dot Blot, Immunoprecipitation (IP), Immunolocalization (IL), Western Blot (WB)
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Expected Species:
Chlamydomonas reinhardtii, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
full length recombinant ycf4 protein of Chlamydomonas reinhardtii UniProt: O20030 as described in Boudreau et al. 1997
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Expected Species:
Algae, Arabidopsis thaliana, Avena sativa, Chlorella vulgaris, Marchantia polymorpha, Phaseolus vulgaris, Physcomitrium patens, Chlorokybus atmophyticus, Ostreococcus tauriSpecies of your interest not listed? Contact us
Immunogen:
full length recombinant ycf3 protein of Chlamydomonas reinhardtii UniProt: O20031, as described in Boudreau et al. 1997
Western blot detection image can be found in Boudreau et al. 1997.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
19 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Epub 2016 Feb 22.Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
Xylan is a group of hemicelluloses that reside in plant cells walls and also can be found in some algae (both green and red). Xylans are polysaccharides whose backbone consists of beta-1,4-linked xylosyl residues. This backbone can be substituted with side-chains of arabinosyl, glucuronosyl, and 4-O-mthylglucuronosyl residues, and can also be further modified by acetyl substitution on the hydroxyls of the xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250
Application Details:
1 g/ml (ELISA), 2 g/10 ml incubation buffer (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10-100 for various glycoproteins
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Xavier et al. (2021) Inactivation of N-Acetylglucosaminyltransferase I and a1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans. Frontiers in Plant Science. Volume 12. doi: 10.3389/fpls.2021.634023Yang et al. (2021). Golgi-localized manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytol. 2021 Jan 17. doi: 10.1111/nph.17209. Epub ahead of print. PMID: 33454966.Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620. Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620.Hou et al. (2019). Identification and characterization of a novel glycoprotein core xylosidase from the bacterium Elizabethkingia meningoseptica. Biochemical and Biophysical Research Communications Available online 27 July 2019.
Special application note:
Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at +4°C(short term) and at -20 °C (long term).
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at +4°C(short term) and at -20 °C (long term).
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598.
Special application note:
Contains 0.05% Sodium Azide.Reacts with galactosylated XLLG oligosaccharide motif of plants xyloglucan.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at +4°C(short term) and at -20 °C (long term).
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ruprecht et al. (2017). A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies. Plant Physiol. 2017 Nov;175(3):1094-1104. doi: 10.1104/pp.17.00737.
Special application note:
Contains 0.05% Sodium AzideReacts with the XXXG motif of land plants xyloglucan. This antibody is directed toward the nonreducing end, with the requirement for the second to last Glc to be substituted with an a-1,6-linked Xyl Ruprecht et al. (2017).
Xyloglucans are polysaccharides commonly referred to as hemicelluloses found in the primary cell walls of vascular plants. Species of trees known as sycamore include: Acer pseudoplatanus, Ficus sycomorus, Platanus orientalis,Platanus occidentalis,Platanus racemosa,Platanus wrightii.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store up to 1 month at 4 C, later at -80°C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Fucosylated xyloglucan, epitope XXFG
Immunogen:
BSA-conjugated (covalently) xyloglucan of Acer pseudoplatanus
Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at +4°C(short term) and at -20 °C (long term).
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store up to 1 month at 4 C, later at -80°C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Xylan (low arab) from Phormium tenax, Phormium spp.
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store up to 1 month at 4 C, later at -80°C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Phormium cookianum, Sorghum bicolor, Zea mays
Immunogen:
High arabinose xylan from Phormium cookianum conjugated to Me-BSA
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Avena sativa
Expected Species:
Higher plantsSpecies of your interest not listed? Contact us
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Betula sp., Eucalyptus sp., Populus sp., Sorghum sp., Triticum sp.
Expected Species:
Higher plantsSpecies of your interest not listed? Contact us
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Higher plantsSpecies of your interest not listed? Contact us
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Xylans are polysaccharides and belong to a group of hemicelluloses found in cell walls of plants and in red and green algae. Their backbones are built by beta-1,4-linked xylosyl residues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare, Oryza sativa
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.Liu et al. (2013). Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9(8): e1003704. doi:10.1371/journal.pgen.1003704 (Oryza sativa, western blot)Hrmova et al. (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)-β. J. Biol. Chem. 82: 12951-12962.
XLG2 protein is involved in G protein-coupled receptor signaling pathway and is involved in defense response, hypersensitive response and response to bacterium.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Recombinant XLG2 of Arabidopsis thaliana UniProt: C6KIE6, TAIR: AT4G34390
The antibody is also recognizing a recombinant XLG2 protein. Reactivity to XLG1 has not been confirmed.
Application Details:
1 : 1000 - 1: 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile or deionized water.
Molecular Weight:
97 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
To be added when available, antibody available in March 2022.
UniProt number:
C6KIE6
TAIR number:
AT4G34390
Research area:
Arabidopsis thaliana antibodies
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