Highlighted Antibody - Melan A Clone BS52
Melan A (MART-1) is a transmembrane protein which is expressed in skin melanocytes and melanocyte lineages, and is ecognised by autologous cytotoxic T lymphocytes.
This antibody is useful for the identification of melanomas and it should be included in standard melanoma diagnostic panels. It does not cross react with cells of adrenal cortex.
KEY ADVANTAGES OF CLONE BS52 (MELAN A ANTIBODY)
• The specificity and stain intensity is top quality.
• Clone BS52 does not stain adrenal gland.
• Easier to optimise than other MELAN A Antibodies.
• This antibody truly provides optimal staining and as with all our antibodies we provide a 100% guarantee, so trying it really is risk free.
Suggested Protocol after paraffin removal and rehydration:
1. Pretreatment: HIER pH9 2. Wash (TBS-Tween) 3. Primary antibody: Melan A 1:100 – 1:400, 30 min. 4. Wash 5. 3% H2O2, 10 min 6. Wash 7. HRP One-Step Polymer, 30 min 8. Wash 9. DAB high contrast Kit, 10 min 10. Aqua 11. CuSO4 -post enhancement, 5 min 12. Aqua 13. Counter staining in diluted Mayer, 1 min 14. Bluing, 7 min in tap water 15. Dehydration, clearing and mounting
Optibodies™ are mouse and rabbit monoclonal antibodies that have been developed specifically for use in immunohistochemistry to have high affinity and high specificity to their target antigens, give a high signal to noise ratio and can be used to achieve an intensive and specific staining.
Please visit our optibodies page and follow the individual product links for more information about the full optibodies range.
Optimal results for Optibodies™ in External QC assessments!
In recent quality assessments by External QC service providers, optimal staining results were obtained for EIGHT different Optibodies clones: CK5 - Cytokeratin 5 (Clone BSR55) EpCAM - Epithelial cell-cell adhesion molecule (Clone BS14) PDL-1 - Programmed Death Ligand 1 (Clone BSR90) SOX10 - Transcription factor SOX-10 (Clone BS7)
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