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Product Search Results: SPC-103D

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Product Search Results: SPC-103D

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StressMarq SPC-103D 100 µl £260.00
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Product Detail: This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info: HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1, 2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (5).
All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Product Type: Antibodies
Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temp: -20ºC
Host Animal: Rabbit
Species Reactivity: Hu | Ms | Rt | Bg | Bv | Dg | Fs | Cp | GP | Hm | Mk | Pg | Sh | Co | Pl | Shk | L. am
Immunogen: Full length protein HSP70
Applications: WB | IHC | ICC/IF | IP | ELISA
Application Details: WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s): Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers
Target: HSP70
Conjugate: Unconjugated
Alternative Name(s): HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Category: Polyclonal
Immunogen Species: Human
Accession Number: NP_005336.3
Gene ID: 3303
Swiss-Prot: P0DMV8/P0DMV9
Species Reactivity Full Name: Human | Mouse | Rat | Beluga (Delphinapterus leucas) | Bovine | Dog | Fish | Carp (Cypriniformes) | Guinea Pig (Cavia porcellus) | Hamster | Monkey | Pig | Sheep | Coral (Anthozoa) | Plant | Tomato | Tobacco | Shark | Spiny Dogfish Shark (Squalus acanthias) | Atlantic Hagfish (Myxine glutinosa) | Leishmania amazonensis
Purification: Peptide Affinity Purified
Concentration: 1 mg/ml
Specificity: Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.
Certificate of Analysis: A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization: Cytoplasm
References: 1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17.
3. Rothman J. (1989) Cell 59: 591 -601.
4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
7. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043.
8. Locke M. (2000) Cell Stress & Chaperones 5: 45-51.
9. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66.
10.Trentin G.A. et al. (2001) J Biol Chem. 276: 13087-13095.
Tarriff Code: 3002.15.0000
ADR Code: Non-hazardous
UN Code for transport: Non-hazardous
Country of Origin: Canada
Cite this Product: StressMarq Biosciences Cat# SPC-103D, RRID: AB_2119380