Newmarket Scientific
Newmarket Scientific








Exosome and Microvesicle Research


Exosome isolation and quantification, exosome standards, antibodies and RNA extraction


Hansabiomed logoExosomics logo

 


 

Our Extracellular Vesicle Research range includes exosome isolation and purification tools, antibodies to microvesicles (that have actually been tested with exosomes in each application) and our lyophilised exosome standards.

 

• High quality purified exosomes • Easy to ship and store (+4°C) • Stable for long term storage (up to 36 months) • Easy to reconstitute • Exosomes are available from a large cell line bank (over 100 cell lines).

 

Some exosome products are highlighted below, followed by a more in depth list further down but please use the search box above if you are looking for a specific product or email us.

 

More information on exosome research including highlighted papers and other resources is available on our Exosome Twitter feed: .

 



evGAG Fast EV Isolation for Biomarker Discovery

 

evGAG is based on an interaction with glycosaminoglycans (GAGs) in the extracellular vesicles (EVs).

 

Provides a higher EV concentration and lower uromodulin contamination (Tamm–Horsfall protein).

 

Works well for human and animal biofluids, including urine samples.

 

Compatible with PCR, qPCR, ddPCR, NGS & Western Blotting.

evGAG Extracellular Vesicle Purification Workflow

 

Further information and pricing is available here.

 



EV-Spinner Ultrafiltration Concentrator for Extracellular Vesicles

 

The EV-Spinner from HansaBioMed is a 2.5 ml non-stick ultrafiltration concentrator designed to enhance the recovery of extracellular vesicles (EVs) and nanoparticles.

 

It offers higher recovery than V shaped concentrators due to its low protein binding membrane that works in the opposite direction to the centrifugal force of the spinner ensuring the filter does not clog.

 

 

Further information and pricing is available here.

 



PURE-EVs size exclusion chromatography columns

 

PURE-EVs size exclusion chromatography columns have been designed specifically for isolating and purifying extracellular vesicles from small volume samples (100 ul - 20 ml). The unique composition of the matrix gives you better purity and recovery yields of your EVs compared to commonly used Semaphore CL-2B SEC columns.

 

The simple and quick protocol (just 15 minutes) is perfect for isolating Extracellular Vesicles (EVs) from biofluids and cell media and purifying pre-isolated EVs from contaminants, because it preserves the original shape and functionality of the vesicles.

 

We have 3 columns for EV purification: PURE-EVs, miniPURE-EVs & maxi-PURE-EVs for use with a range of starting volumes. Plus our PURE-EVs PLUS system, provides Pure-EV columns with our TFF-Easy concentrator for concentrating EVs from larger volumes of dilute fluids and preparations, before purifying them using the columns.

 

                     PURE-EVs for Exosome Purification       PURE-EVs PLUS for Exosome concentration and Purification



Quickly concentrate EV samples with TFF-Easy

 

HansaBioMed's TFF-Easy concentrator can be used to concentrate extracellular vesicles (EVs) and remove small proteins from diluted matrices such as cell conditioned media, urine or blood samples prior to EV purification or dialysis.

 

The TFF-Easy concentrator is a filter cartridge made from polysulfone hollow fibres with 20 nm pores that uses constant agitation parallel to the porous membrane to allow the gentle filtration of the EVs whilst avoiding clogging issues. The EV protocol is simple and fast, providing a high recovery rate and it does not require any... read more and view the TFF-Easy Video.

 

TFF-Easy Video

 



Purifying microvesicles - Save time with the TFF-MV Concentrator

 

HansaBioMed’s TFF-MV concentrator allows one-step purification of microvesicles from large volume samples from just a few millilitres to several litres. With a filter cartridge made from polysulfone hollow fibers it can separate different extracellular vesicles (EVs) by size with no clogging. Water, small molecules and small EVs pass through the hollow fibre pores, leaving larger microvesicles (>150 nm) in the cartridge. The larger microvesicles can then be... read more about the TFF-easy and TF-MV concentrators.

 

TFF-easy and TFF-MV concentrators

 



ExoPrep: For Faster Exosome Isolation

 

EXO-Prep is an efficient method for exosome isolation from biofluids (i.e plasma serum and urine) or cell culture supernatant.

 

EXO-Prep - Exosome Isolation

 



Extracellular Vesicle Research Tools Overview

 

Exosome and Microvesicle Standards

   Lyophilized exosomes from PLASMA

   Lyophilized exosomes from URINE

   Lyophilized exosomes from SERUM

   Lyophilized exosomes from MESENCHYMAL STEM CELLS

   Lyophilized exosomes from MEDIA (cell culture supernatant)

   Lyophilized plant derived ELNs

   Lyophilized exosomes from Chinese Hamster Ovary (CHO)

   Lyophilized MICROVESICLES

   Lyophilized Exosomes and Microvesicles from MOUSE CELL LINES

 

 

EV Isolation: PURE-EV Size exclusion chromatography columns

   PURE-EV columns (sample volume 500ul-2mL)

   miniPURE-EV columns (sample volume 100ul-500ul)

   maxiPURE-EV columns (sample volume 5ml-20ml)

 

EV Isolation: TFF Filters and EV Spinner

   TFF-Easy - tangential flow exosome filtration concentrator

   TFF-MV - one step purification of microvesicles from large volume samples up to litres

   EV-Spinner: Ultrafiltration Concentrator

 

EV Isolation: EXO-Prep: Exosome Isolation

   EXO-Prep One Step Exosome Isolation

 

Immunoaffinity EV capture

   Immunoplates

   Immunobeads

 

Quantification Kits

   ExoTEST - Exosome capture & quantification from PLASMA, URINE, SERUM, MEDIA

   ExoTEST - Tumor Exosome enrichment & quantification from PLASMA

 

FACS Analysis of Exosomes (Exo-FACS Kits)

   Kits for analysis of exosomes from PLASMA, URINE, SERUM, SALIVA, MEDIA

 

Kits for DNA/RNA extraction from Exosomes

   Basic Exosome RNA Kits

 

Exosome Related Antibodies

   Anti CD9 Antibodies

   Anti CD63 Antibodies

   Anti CD41 Antibodies

   Anti CD44 Antibodies

   Anti CD81 Antibodies

   Anti Caveolin1 Antibodies

   Anti ALIX Antibodies

   Anti TM9SF4 Antibodies

   Anti flotillin Antibodies

   Anti Rab5 Antibodies

   Anti HSP70 Antibodies

   Anti TSG101 Antibodies