| Background Info: |
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays |
| Expected Species: |
Glycine max, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Zosteria marinaSpecies of your interest not listed? Contact us |
| Immunogen: |
Native purified 33 kDa protein from Spinacia oleracea UniProt: P12359 |
| Applications: |
Immunoprecipitation (IP), Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007). |
| Application Details: |
1 : 2000-1 : 5000 (WB) |
| Purity: |
Total IgG. Protein G purified in PBS pH 7.4. |
| Reconstitution: |
For reconstitution add 50 l of sterile water |
| Molecular Weight: |
35 | 33 kDa |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
| Selected references: |
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..Tang el al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.Smythers et al. (2019). Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism. Chemosphere Volume 219, March 2019, Pages 704-712. |
| Special application note: |
Total IgG fraction has been purified by 40% ammonium sulpgate precipitation followed by DEAE cellulose chromatographyThis product can be sold containing ProClin if requested |
| Research area: |
PSII (Photosystem II) |
| Code: |
Photo20 CM20 |
