| Product Detail: |
nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody |
| Background Info: |
Raised against synthetic peptide sequence corresponding to amino acids 134-148 of human nNOS coupled to KLH. |
| Product Type: |
Antibody - Antibodies |
| Antibody Type: |
polyclonal |
| Format: |
Lyophilised |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Rat |
| Immunogen: |
Human nNOS (134-148) |
| Applications: |
Immunohistochemistry, Immunocytochemistry, immunofluorescence, Western Blot |
| Data Sheet: |
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| Additional Info: |
The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed. |
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