Newmarket Scientific Product Search Results for 24431

Product Search Results: 24431
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Product Search Results: 24431	Num Products Matched: 3	3

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ImmunoStar

24447

25 ug

£99.00

ImmunoStar

24431

100 uL

£372.00

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Product Detail:	nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody
Background Info:	Raised against synthetic peptide sequence corresponding to amino acids 134-148 of human nNOS coupled to KLH.
Product Type:	Antibody - Antibodies
Antibody Type:	polyclonal
Format:	Lyophilised
Host Animal:	Rabbit
Species Reactivity:	Mouse, Rat
Immunogen:	Human nNOS (134-148)
Applications:	Immunohistochemistry, Immunocytochemistry, immunofluorescence, Western Blot
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Additional Info:	The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.
PubMed IDs:	22396407, 21280041, 19105198, 16807372, 16697052

Agrisera

AS21 4545

100 µl

£348.00

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Background Info:	E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.
Product Type:	Antibody
Antibody Type:	Polyclonal
Format:	Liquid
Storage Temp:	Store at 4°Cfor 6 monthss, afterwards at -80 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:	Rabbit
Species Reactivity:	Escherichia coli
Immunogen:	Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814
Applications:	Western blot (WB)
Data Sheet:	PLEASE CLICK HERE TO DOWNLOAD
Application Details:	1 : 3000 (WB)
Purity:	Serum. Contains 0.05 % sodium azide.
Molecular Weight:	19 kDa (forms dimer in liquid)
Selected references:	Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.
Research area:	Bacterial antibodies

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