Anti-ATP5H in Antibody Picoband (monoclonal, 6B12)
Antibody Type:
Monoclonal
Host Animal:
Mouse
Species Reactivity:
Human,Monkey,Mouse,Rat
Immunogen:
E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500?g/ml. Background: ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Colemanosphaera charkowiensis, Eudorina elegans,Gonium pectorale, Pandorina colemaniae, Pleodorina starrii, Volvox africanus, Yamagishiella unicocca Species of your interest not listed? Contact us
Immunogen:
CF 1 alpha subunit of the chloroplast ATP synthase complex isolated from Chlamydomonas reinhardtii, UniProt: P26526
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Avena sativa
Expected Species:
higher plants
Immunogen:
V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
CGL160 (conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF1 sub-complex and is required for the efficient incorporation of the c-subunit into the cpATPase. Synonymes: ATCGL160.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Fristedt et al. (2015). The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
PGR5 (Protn gradient regulation 5) is involved in the regulation of the cyclic electron flow (CEF) around Photosystem I and cellular response to light intensity and photoprotection. Essential for the reduction of PGRL1A by ferredoxin and for photoprotection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing proclin if requested
Application Details:
1: 1000 - 1: 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
14,29 kDa
Not reactive in:
diatoms, Chlorella sp., cyanobacteria
Selected references:
Cao et al. (2022) Autophagic pathway contributes to low-nitrogen tolerance by optimizing nitrogen uptake and utilization in tomato. Hortic Res. 2022 Mar 23;9:uhac068. doi: 10.1093/hr/uhac068. PMID: 35669705; PMCID: PMC9164271.Ermakova et al. (2022) Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot. 2022 Jul 29:erac320. doi: 10.1093/jxb/erac320. Epub ahead of print. PMID: 35904136.Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105026Yang et al. (2020). Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring. Nat Commun. 2020 Jan 8;11(1):128. doi: 10.1038/s41467-019-13954-0.Rantala et al. (2020). PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861(3):148154. doi: 10.1016/j.bbabio.2020.148154.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. This enzyme is localised in mitochondrial inner membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. botrytis cv. 'Diadom'
Expected Species:
Chlamydomonas reinhardtii, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal mitochondrial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana ATP synthase subunit beta-1 UniProt:P83483, TAIR:AT5G08670ATP synthases subunit beta-2 UniProt:P83484, TAIR: AT5G08690, ATPase subunit beta-3, UniProt: Q9C5A9, TAIR:AT5G08680, which belong to mitochondrial respiratory chain complex I.
Wei et al. (2019). Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis. J Exp Bot. 2019 Aug 6. pii: erz357. doi: 10.1093/jxb/erz357.
Special application note:
Lack of antibody reactivity was confirmed on chloroplast fraction.This product can be sold containing ProClin if requested.
PAB (protein in chloroplast atpase biogenesis) acts as an assembly chaperone that functions downstream of chaperonin 60 in the assembly of chloroplast ATP synthase coupling factor 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidiopsis thaliana, Zea mays
Expected Species:
Brachypodium distachyon, Gossypium raimondii, Hordeum vulgare, Oryza sp., Saccharum hybrid cultivar R570, Setaria italica, Sorghum bicolor, Theobroma cacao, Triticum aestivum Species of your interest not listed? Contact us
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gao et al. (2018). A supercomplex, approximately 720 kDa and composed of both photosystem reaction centers, dissipates excess energy by PSI in green macroalgae under salt stress. Plant Cell Physiol. 2018 Oct 8. doi: 10.1093/pcp/pcy201.Koochak et al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127. (BN-PAGE)Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.
Special application note:
This product can be sold containing proClin if requested
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at at Store short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended.
This product can be sold with ProClin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Molecular Weight:
23 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Blair et al. (2018). The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton. Mol Microbiol. 2018 Jul 24. doi: 10.1111/mmi.14087.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus lucimarinusm, Spinacia oleracea, Sorghum bicolor, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii Q42687.1
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Perlaza (2021). Organelle Size and Quality Control in Chlamydomonas Reinhardtii. UCSF. ProQuest ID: Perlaza_ucsf_0034D_12217. Merritt ID: ark:/13030/m5257z1d. Retrieved from https://escholarship.org/uc/item/1jg3874hPerlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577. (immunofluorescence)
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
dicots, Chlamydomonas reinhardtii, cyanobacteria including Synechocystis sp. PCC 6803.
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane CF0IV subunit of appr. 25 kDa belongs to a stator part of ATP synthase and is involved in the proton translocation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Cannabis sativa, Glycine max, Hordeum vulgare, Oryza sativa, Ostreococcus tauri, Physcomitrium patens, Pinus thunbergii, Pisum sativum, Populus alba, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana UniProt: P56760, TAIR: AtCg00140 and Chlamydomonas reinhardtii UniProt: Q37304
Please note that increased incubation at 95 C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
8 kDa (for Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schulz et al. (2017). Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei. EMBO Rep. 2017 Apr;18(4):526-535. doi: 10.15252/embr.201643374.
Special application note:
This product can be sold containing ProClin if requested
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Application Details:
1: 600 (IF), 1 : 1000-1 : 3000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
26 | 31 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. , Physcomitrium patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Application Details:
1 : 1000-1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 300 l of sterile water
Molecular Weight:
26 | 31 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Special application note:
This product can be sold containing ProClin if requested
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1, TAIR: At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.
VIPP1 - vesicle-inducing protein in plastids 1, is a hydrophilic found in both the inner envelope and the thylakoid membranes. In VIPP1 deletion mutants vesicle formation is abolished. VIPP1 is proposed to be essential for the maintenance of thylakoids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Synechococcus sp. Species of your interest not listed? Contact us
Immunogen:
Recombinant VIPP1 of Chlamydomonas reinhardtii, UniProt: Q66YD0
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Kroll et al. (2001) VIPP1, a nuclear gene of Arabidopsis thaliana essential for thylakoid membrane formation. Proc Natl Acad Sci U S A 98: 4238-4242.
Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Catalpa bungei, Dicots, Chlamydomonas reinhardtii, Nicotiana benthamiana, Physcomitrium patens, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
Purified native plastocyanin from Spinacia oleracea UniProt: P00289
Plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.
Application Details:
1 : 100 (IG), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Viola et al. (2021) In vivo electron donation from plastocyanin and cytochrome c6 to PSI in Synechocystis sp. PCC6803. Biochim Biophys Acta Bioenerg. 2021 May 15;1862(9):148449. doi: 10.1016/j.bbabio.2021.148449. Epub ahead of print. PMID: 34004195.Furutani et al. (2021) The difficulty of estimating the electron transport rate at photosystem I. J Plant Res. 2021 Nov 15. doi: 10.1007/s10265-021-01357-6. Epub ahead of print. PMID: 34778922.Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.
Special application note:
Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested.
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.
Application Details:
1 : 2000-1 : 50 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
34 | 31-32 kDa
Selected references:
Redekop et al. (2020). PsbS Contributes to Photoprotection in Chlamydomonas Reinhardtii Independently of Energy Dissipation . Biochim Biophys Acta Bioenerg . 2020 Jun 1;1861(5-6):148183.doi: 10.1016Liu et al. (2020). Acid treatment combined with high light leads to increased removal efficiency of Ulva prolifera. Algal Research,Volume 45, January 2020, 101745Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Koh et al. (2019). Heterologous synthesis of chlorophyll ? b ? in ? Nannochloropsis salina ? enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels. ? 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3. eCollection 2019.Dall'Osto et al. (2019). Combined resistance to oxidative stress and reduced antenna size enhance light-to-biomass conversion efficiency in Chlorella vulgaris cultures.Biotechnol Biofuels. 2019 Sep 16;12:221. doi: 10.1186/s13068-019-1566-9.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Storti et al. (2018). Role of cyclic and pseudo-cyclic electron transport in response to dynamic light changes in Physcomitrella patens. Plant Cell Environ. 2018 Nov 29. doi: 10.1111/pce.13493.Kong et al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.Sch ttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. ? 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Zou et al. (2017). An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle. Plant Physiol. 2017 Jul;174(3):1334-1347. doi: 10.1104/pp.17.00493.Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. (Synechocystis PCC6803) Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939.Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004.Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Catalpa bungei, Micromonas sp. , Populus trichocarpa, Physcomitrium patens, Ricinus communisSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.
Schmid et al. (2018). PUMPKIN, the sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism. Plant Physiol. 2018 Nov 8. pii: pp.00687.2018. doi: 10.1104/pp.18.00687.Patil et al. (2018). FZL is primarily localized to the inner chloroplast membrane however influences thylakoid maintenance. Plant Mol Biol. 2018 Jul;97(4-5):421-433. doi: 10.1007/s11103-018-0748-3.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Kanazawa et al. (2017). Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection. Front Plant Sci. 2017 May 3;8:719. doi: 10.3389/fpls.2017.00719.Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 g/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.
Special application note:
This product can be sold containing proClin if requested.The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Ultrastructure Expansion Microscopy (U-ExM), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 g/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Ripamonti et al (2022). Silencing of ATP Synthase Beta Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Doree. International Journal of Molecular Sciences. 2022; 23(2):765. https://doi.org/10.3390/ijms23020765Tanno et al. (2021) The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. PLoS One. 2021 Oct 26;16(10):e0259138. doi: 10.1371/journal.pone.0259138. PMID: 34699573; PMCID: PMC8547699.
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Manihot esculenta, Medicago sativa, Nannochloropsis sp., Pisum sativum, Triticum aestivum, cellular [compartment marker] of thylakoid membrane. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.
Application Details:
1 : 15 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
38 | 28-30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.Sorrentino et al. (2018). Performance of three cardoon cultivars in an industrial heavy metal-contaminated soil: Effects on morphology, cytology and photosynthesis. J Hazard Mater. 2018 Jun 5;351:131-137. doi: 10.1016/j.jhazmat.2018.02.044.Kanazawa et al. (2017). Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection. Front Plant Sci. 2017 May 3;8:719. doi: 10.3389/fpls.2017.00719.Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016
Special application note:
Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Purified c subunit from Spinacia oleracea UniProt: P69447.
Note that increased incubation at 95 C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
Application Details:
1 : 1000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
8 kDa (for Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
Special application note:
This product can be sold containing proclin if requested.
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product Type:
Antibody
Format:
Lyophilized, in glycerol.
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 90 l of dest. water final concentration of the standard is 0.27 pmol/ l.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 90 l of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
in most gel systems AtpB migrates around 50-54 kDa
Selected references:
Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLOS ONE.
Special application note:
The AtpB protein standard can be used in combination with global anti-AtpB antibodies to quantitate AtpB from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the AtpB protein.Quantitative western blot: detailed method description, video tutorial
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 23,3 g/ l
Storage Temp:
Store at 2-8°C.; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Immunolocalization (IL), Immunogold (IG), Western blot (WB)
Neusius et al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Nelson et al. (2019). Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. doi: 10.1111/pce.13692.Gellert et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005. (immunogold)Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13.
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
Applications:
Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)
This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, (AS03 037) combined with Rubisco ready to use standard (AS01 017) is recommended.
Application Details:
(IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 g of total cellular protein, (WB)
Purity:
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Guljamow et al. (2021) Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806. Microorganisms. 2021 Jun 10;9(6):1265. doi: 10.3390/microorganisms9061265. PMID: 34200971; PMCID: PMC8230624. (IF)Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1–20. (sea-ice diatom)Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gell rt et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005.Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.
Special application note:
Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria
Rat Atp5b(ATP synthase subunit beta, mitochondrial) ELISA Kit
Product Type:
Assay & Detection
Storage Temp:
4°C
Applications:
ELISA
Biosite Brand:
BioSite ELISA
Species Reactivity:
rat
UniProt No:
P10719
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