CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
living human myeloid cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
living human myeloid cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
Clone number:
CB38
Antibody Isotype:
IgM k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
living human myeloid cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
Clone number:
CB38
Antibody Isotype:
IgM k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
living human myeloid cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
Clone number:
CB38
Antibody Isotype:
IgM k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of human IRAK4 expressed in E. Coli.
E-cadherin is a Ca2+-dependent, transmembrane cell adhesion molecule. It plays an important role in the growth, development and the intercellular adhesion of epithelial cells. Most tumors have an abnormal architecture and any subsequent loss of adhesiveness is thought to be an important step in the development of local invasion. E-cadherin may have a role in neoplastic progression, particularly as a suppressor of invasion. In prostate cancers, for example, the expression of E-cadherin is reported to be reduced or absent in comparison with its expression in normal prostate which is uniformly strong. Reduced expression or absence of E-cadherin in addition to alpha, beta and gamma-catenin in primary breast carcinomas has also been reported and these four proteins are associated with the development of metastases.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
36B5
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with Sodium azide
Storage:
2-8°C
References 1:
Elston MS et al. J.of Clin.Endocrinology and Metabolism. 2009; 94(4):1436-1442.
References 2:
Munhoz NG et al. The Open Pathology Journal. 2009; 3:10-17
References 3:
Chetty R and Serra S. Histopathology 2008; 52: 325330
References 4:
Schott M et al. Endocrinology and Metabolism 2007; 92(9):3378- 3382
References 5:
Dansranjavin T et al. Oncology Reports. 2006; 15:1125-1131
Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of ?, ?, and ? chains. A5 reacts specifically with ? chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov JY et. al. Cancer Res. 61(14): 5601-5610 (2001)
References 2:
Ljubimov AV et. al. Int J Cancer 50: 562-566 (1992)
9-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the d epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 9-13M1 and 2-11M1 react exclusively with epitopes located in the the Nterminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
9-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
58M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the e epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
58M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
45M1 recognizes the peptide core of gastric mucin M1 (now: MUC5AC), and more specifically with the h epitope amongst the a, b, c, d, e, f, g and h protein core epitopes defined by Bara for M1. 45M1 and 2-12M1 both specifically react with epitopes located in the C-terminal cysteine rich part of the peptide core of MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
45M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 2:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
2-12M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the c epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-12M1 and 45M1 both specifically react with epitopes located in the Cterminal cysteine rich part of the peptide core of gastric mucin (MUC5AC). MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. AntiMUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-12M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
2-11M1 recognizes the peptide core of gastric mucin M1/MUC5AC, and more specifically with the b epitope amongst the a, b, c, d, e, f, g, and h protein core epitopes defined by Bara for M1. 2-11M1 and 9-13M1 react exclusively with epitopes located in the N-terminal cysteine-rich part of the peptide core MUC5AC. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
2-11M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
1-13M1 recognizes the peptide core of gastric mucin M1/MUC5AC), and more specifically with the a epitope, which is the most abundant amongst the a, b, c, d, e, f, and h protein core epitopes defined by Bara for M1. MUC5AC is present in primary ovarian mucinous cancer and gastric cancer, but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification pancreatic carcinoma and precancerous changes vs. normal pancreas.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
1-13M1
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bara, J. et al., Cancer Res.46: 3983-3989 (1986)
References 2:
Bara, J. et al., Biochem. J. 254: 185-193 (1988)
References 3:
Bara, J. et al., Int. J. Cancer 47: 304-310 (1991)
References 4:
Bara, J. et al., J. Immunol. Methods 149: 105-113 (1992)
References 5:
Guyonnet Duperat V. et al., Biochem. J. 305: 211 219 (1995)
The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.<br /> The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.<br /> MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.<br /> Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
15-Feb
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Barret-Berghoeff; M et al. Thromb Haemostas 1997; 77: 718
The antibody produces strong labeling of VAChT at dilutions of 1/200 - 1/400 using indirect immunofluorescence and at dilutions of 1/3,000 - 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Lyophilised
Host Animal:
Goat
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rat VAChT 511-530
Applications:
Electron Microscopy; Immunocytochemistry; Immunofluorescence; Immunohistochemistry.
Solute carrier family 18, member 3;solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3;rVAT; VACht
Additional Info:
The VAT Antibody produces strong labeling of VAChT at a dilution of 1/3,000 - 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain.
Gene Symbol:
Slc18a3,60422
NCBI Gene Aliases:
Solute carrier family 18, member 3; member 3;rVAT; VACht
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Vedyaykin et al. (2020). SulA is able to block cell division in Escherichia coli by a mechanism different from sequestration. Biochem Biophys Res Commun . DOI: 10.1016/j.bbrc.2020.03.012 Ranjit et al. (2020). Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11(1). pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence)Sekar et al. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.M ckl et al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.S derstr m et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
RbcL antibody and protein standard: Please store at -20 °C (6 months) or -80°Cfor long term storage(years). Please avoid freezing and thawing of reconstituted antibodies, make aliquots instead. PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°Cand for long term storage (1 year) at -20 °C.
Alpha proteobacteria, Algae (brown and red), Beta-proteobacteria, Dicots, Conifers, Cryptomonad, Cyanobacteria, Gamma-proeobacteria, Liverworts, Mosses, Prochlorophytes, PelwitschiaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
1 x 50 µl of AS03 037, RbcL | Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 34 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)2 x 2 ml of AS08 300, Protein extraction buffer (amount enough for 48 isolations of plant material, using 500 µl 1x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)2 x 10 µl of AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated(amount enough for 50-100 Western blots)1 X 10 ml of AS16 ECL-N-10, AgriseraECL Bright 10 ml (5 ml of component A + 5 ml of component B, trial pack)
Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.
Defez et al. (2019). Bacterial IAA-Delivery into Medicago Root Nodules Triggers a Balanced Stimulation of C and N Metabolism Leading to a Biomass Increase. Microorganisms. 2019 Sep 29;7(10). pii: E403. doi: 10.3390/microorganisms7100403.Sorrentino et al. (2018). Performance of three cardoon cultivars in an industrial heavy metal-contaminated soil: Effects on morphology, cytology and photosynthesis. J Hazard Mater. 2018 Jun 5;351:131-137. doi: 10.1016/j.jhazmat.2018.02.044.Mota et al. (2015). Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles. J Proteomics. 2015 Apr 29;120:75-94. doi: 10.1016/j.jprot.2015.03.004.Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Supplemental material describes western blot quantification method.
Research area:
Global Antibodies
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