Background Info: |
FBA (Fructose-bisphosphate aldolase, chloroplastic) is an enzyme involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose. |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidopsis thaliana, Chrysanthemum seticuspe (Gojo-0), Festuca arundinacea, Lolium multiflorum, Oryza sativa |
Expected Species: |
Aegilops tauschii, Arundo donax, Avena sativa, Brachypodium distachyon, Cajanus cajan, Catharanthus roseus, Carica papaya , Citrus sinensis, Cucumis sativus, Eucalyptus grandis, Genlisea aurea, Glycine soja , Gossypium raimondii, Hordeum vulgare var. distichum, Jatropha curcas, Leersia perrieri, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Trifolium pRatense, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated peptide chosen from chloroplast fructose-1,6-bisphosphate aldolase (FBA) EC 4.1.2.13 chosen from Brachypodium distachyon FBA protein sequence. UniProt: I1IN47 |
Applications: |
Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
Standard extraction protocol for leaf tissue can be applied. The antibody is directed on the unique peptide present in a chloroplast form of aldolase; it does not react with a cytosolic form in the Lolium-Festuca species. |
Application Details: |
1 :4000-1 : 8000 (WB) |
Purity: |
Serum |
Reconstitution: |
For reconstitution add 50 l of sterile water |
Molecular Weight: |
42 | 38 kDa |
Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
Selected references: |
Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.Perlikowski et al. (2016). Water deficit affects primary metabolism differently in two Lolium multiflorum/Festuca arundinacea introgression forms with a distinct capacity for photosynthesis and membrane regeneration. Frontiers in Plant Science 7:1063. doi: 10.3389/fpls.2016.01063 |
Special application note: |
This product can be sold containing ProClin if requested. |
Research area: |
Carbohydrates |
|
Background Info: |
Plant MnSOD catalyzes the dismutation of superoxide to molecular oxygen and water. One subunit of the homotetramer complex binds 1 Mn cofactor. Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD. Synonymes: MSD1, SODA, SOD3 |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidopsis thaliana, Armeria maritima, Brassica napus, Brassica oleracea, Fragaria x ananassa, Iris pumila, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Pisum sativum, Salicornia sp. , Solanum tuberosum,Fragaria x ananassa |
Expected Species: |
Gossipium mexicanum, Hordeum vulgare, Musa acuminata, Picea sitcHensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Solanum tuberosum, Triticum aestivum, Vitis vinifera, Zea maysSpecies of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated synthetic peptide derived from available MnSOD sequences in di and monocotyl plants including Arabidopsis thaliana O81235, At3g10920 |
Applications: |
Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Application Details: |
1 : 2000-1 : 5000 (WB) |
Purity: |
Serum |
Reconstitution: |
For reconstitution add 50 l of sterile water |
Molecular Weight: |
25 | 25 kDa |
Not reactive in: |
algae, Marchantia polymorpha |
Selected references: |
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Bal žov et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe DBal žov rought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066. |
Special application note: |
Freshly prepared reducing agent like DTT needs to be used in a sample buffer, Otherwise MnSOD will migrate at 50 kDa |
UniProt number: |
O81235 |
TAIR number: |
AT3G10920 |
Research area: |
Arabidopsis thaliana antibodies |
Code: |
Stress20 |
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