Product Search Results : NOVA-4234-05


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Bioo Scientific - NGS NOVA-4234-05 96 rxns £4773.00
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Product Detail: The NEXTflex™ Colon-1 Amplicon Panel allows you to quickly target and sequence 36 exons of the MLH1 and MSH2 loci. This kit contains primer pairs and reagents needed to amplify these coding regions of MLH1 and MSH2 from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Colon-1 Amplicon Panel, the panel has the capability of multiplexing up to 700 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane.

A Complete Solution for Targeted MLH1 and MSH2 Sequencing

The NEXTflex Colon-1 Amplicon Panel is a complete, validated solution that targets 36 exons in MLH1 and MSH2 loci using 56 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.

Kit Specs

The NEXTflex™ Colon-1 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

•100% coverage of MLH1 and MSH2 exons and flanking intron-exon boundaries
•98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
•384 unique barcodes available
•Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
•Low input - Only 20 ng DNA isolated from fresh or frozen samples required

This kit is manufactured to the international quality standard ISO 13485.
Product Type: NGS - Illumina Amplicon Panels
Applications: Illumina Colon-1 Amplicon Panel
Search Terms: Colon-1 Amplicon Panel
Application Details: Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4234-05
NGS Applications: Colon-1 Panel