The NEXTflex Small RNA-Seq Kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina sequencing platforms with gel-free or low-input options. Bioo Scientifics approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols. This reduction in bias results in data that more accurately represents abundances of small RNAs in the starting material. In addition, reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.
PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library preparation. This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ?200 ng of total RNA. Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
Replacement for kit 5132-03
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5132-05
The NEXTflex Small RNA-Seq Kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina sequencing platforms with gel-free or low-input options. Bioo Scientifics approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols. This reduction in bias results in data that more accurately represents abundances of small RNAs in the starting material. In addition, reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.
PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library preparation. This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ?200 ng of total RNA. Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The adapter-dimer reduction technology incorporated into this kit also allows low input library preparation. Library preparation with as little as 5 ng of total RNA is possible as additional PCR cycles can be performed without adapter-dimer products dominating the final library. Fig. 2 illustrated that expression values are reproducible across different sample inputs.
Completely gel-free protocol with normal input amounts Greater discovery/detection rates reduces sequencing cost Randomized adapters reduce ligation bias, resulting in more accurate data Low input (5 ng) gel-based protocol included AIR Ligase offers greater sequencing depth Use more of your RNA sample - 10.5 µL input volume Barcoded primers are included in the kit allowing multiplexing of up to 48 samples Functionally validated with Illumina sequencing platform
Replacement for kit 5132-03
The NEXTflex Small RNA Sequencing Kit v3 contains the reagents, including barcoded primers, to prepare 8 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 6 months when stored properly at -20°C. Ships on dry ice.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5132-06
Barcoded PCR Primers for multiplexing contain embedded index sequence Up to 48 multiplexed samples Considerably reduce your per-sample sequencing cost by barcoded multiplexing Increase your sequencing scale by pooling 100s of samples on a single flow cell Compatible with Illumina Next-Generation Sequencing platforms (GAII, HiSeq and MiSeq) Replacement for V1 Small RNA Barcodes
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Small RNA - Illumina Compatible
Applications:
Small RNA Sequencing
Search Terms:
Small RNA Sequencing
Additional Info:
[BENEFITS] >>> Barcoded PCR Primers for multiplexing contain embedded index sequence >>> Up to 48 multiplexed samples >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 513308
Barcoded PCR Primers for multiplexing contain embedded index sequence Up to 48 multiplexed samples Considerably reduce your per-sample sequencing cost by barcoded multiplexing Increase your sequencing scale by pooling 100s of samples on a single flow cell Compatible with Illumina Next-Generation Sequencing platforms (GAII, HiSeq and MiSeq) Replacement for V1 Small RNA Barcodes
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Small RNA - Illumina Compatible
Applications:
Small RNA Sequencing
Search Terms:
Small RNA Sequencing
Additional Info:
[BENEFITS] >>> Barcoded PCR Primers for multiplexing contain embedded index sequence >>> Up to 48 multiplexed samples >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 513307
Barcoded PCR Primers for multiplexing contain embedded index sequence Up to 48 multiplexed samples Considerably reduce your per-sample sequencing cost by barcoded multiplexing Increase your sequencing scale by pooling 100s of samples on a single flow cell Compatible with Illumina Next-Generation Sequencing platforms (GAII, HiSeq and MiSeq) Replacement for V1 Small RNA Barcodes
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Small RNA - Illumina Compatible
Applications:
Small RNA Sequencing
Search Terms:
Small RNA Sequencing
Additional Info:
[BENEFITS] >>> Barcoded PCR Primers for multiplexing contain embedded index sequence >>> Up to 48 multiplexed samples >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 513306
Barcoded PCR Primers for multiplexing contain embedded index sequence Up to 48 multiplexed samples Considerably reduce your per-sample sequencing cost by barcoded multiplexing Increase your sequencing scale by pooling 100s of samples on a single flow cell Compatible with Illumina Next-Generation Sequencing platforms (GAII, HiSeq and MiSeq) Replacement for V1 Small RNA Barcodes
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - Small RNA - Illumina Compatible
Applications:
Small RNA Sequencing
Search Terms:
Small RNA Sequencing
Additional Info:
[BENEFITS] >>> Barcoded PCR Primers for multiplexing contain embedded index sequence >>> Up to 48 multiplexed samples >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please use our contact form above to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 513305
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