Wilms' Tumour Protein (WT1) is a transcription factor involved in the development of the urogenital system. Anti-WT1 is utilized in the differential diagnosis of pulmonary malignancies (nuclei staining) and small round cell tumours. Ewing's sarcomas, primitive neuroectodermal tumours, neuroblastomas, rhabdomyosarcomas, and rhabdoid tumours do not stain with Anti-WT1, but cytoplasmic staining may be observed. Although lung adenocarcinomas do not exhibit nuclear staining with Anti-WT1, the antibody may stain the cytoplasm. Anti-WT1 also stains serous ovarian carcinomas, but does not stain mucinous carcinomas of the ovary and pancreatobiliary carcinomas.
TOM9 (Mitochondrial import receptor subunit TOM9) is a central component of the receptor complex responsible for recognition and translocation of cytosolically synthesized mitochondrial preproteins. Alternative names: Mitochondrial import receptor subunit TOM22 homolog 2, Translocase of outer membrane 22 kDa subunit homolog 2, Translocase of outer membrane 9 kDa subunit TOM9-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Hordeum vulgare, Musa sp., Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zea mays Species of your interest not listed? Contact us
Immunogen:
Recombinant full length protein (coding region) of Tom9.2 Arabidopsis thaliana UniProt: Q9FNC9 TAIR: AT5G43970
Antibody works on whole leaf extracts and isolated mitochondria; requires Tricine gels for sharp bands due to the small MW
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10 | 9 kDa
Not reactive in:
Ostreococcus tauri
Selected references:
Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was confirmed by western blot detecting human superoxide dismutase (SOD1). This antiserum is known to react with human superoxide dismutase (SOD1).
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10,97 | 12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
Mouse anti-Spectrin alpha chain, non-erythrocytic 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. The Spectrins form into dimers and further into tetramers of alpha and beta subunits (Ref: Entrez Gene). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
3D7
Antibody Isotype:
IgG1
Application Details:
WB, ICC, IHC and FC. Recommended dilution of 1:1,000-1:2,000 for ICC and IHC, and 1:5,000-10,000 for WB. The protein is seen as a major band at 240 kDa depending on the species. For Flow Cytometry, use ~ 2 ?g antibody per ~10^6 cells. Optimal concentrations/dilutions should be determined by the end-user.
FUNCTION: Ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for immunohistochemistry and 1:2000 to 1:4000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria
Selected references:
Lhcb2 Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446. Lhcb4Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457. Lhcb5 and Lhcbm5 Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482
The SATB2 [IHC095] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC095
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Colon Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Rabbit anti-Saporin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Plant
Immunogen:
Saporin, whole molecule
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot. A dilution of 1:200 to 1: 2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Saponaria officinalis; Common soapwort
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
S4 (mitochondrial ribosomal small subunit protein S4) is a structural constituent of mitochondrial ribosome. Encoded by RPS4 gene.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 50 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.Kwaśniak et al. (2013). Silencing of nuclear RPS10 gene encoding mitochondrial ribosomal protein alters translation in Arabidopsis mitochondria. Plant Cell 25 (5): 1855-1867.
Special application note:
This product can be sold containing proclin if requested
Mouse anti-Rhodopsin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Purified bovine rhodopsin
Applications:
IHC-Frozen,WB
Clone number:
B630
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000) and Immunohistochemistry (1:1,000). Due to the highly hydrophobic nature of rhodopsin, avoid boiling samples in SDS-PAGE sample buffer for rhodopsin analysis by Western Blotting, as this will result in extensive aggregation of the rhodopsin protein and appearance of high molecular weight bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Bovine,reacts with Human, Rat, Mouse, Cow, Pig. Expected to react with other mammalian species.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Red fluorescent protein (DsRed) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
A monoclonal made againsts recombinant RFP and designed to react specifically against it and its many variants
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Recombinant Red Fluorescent Protein (dsRed) expressed from bacteria.
Applications:
ICC,IHC-Frozen,WB
Clone number:
RF5R
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). Suggested starting dilutions are as follows: WB at 1:1,000, IP at 5 ug/mg lysate and IHC at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RFP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Designed to detect Red Fluorescent Protein (RFP) and its variants in ELISA (sandwich or capture), immunoblotting, immunoprecipitation and immunohistochemistry.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
IBL-3/25 is directed against the ?-chain of CD8. The CD8 complex consists of a disulphide-linked ?/? heterodimer with a MW of 30 kDa or an ?/? homodimer with a MW of 32 kDa. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T-cells with antigenpresenting cells or with target cells. CD8+ T-cells include most of the cytotoxic T-cells.
Antibody Isotype:
IgG-K
Monosan Range:
MONOSAN
Clone:
IBL-3/25
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Knapp W. et. al. Leukocyte Typing IV, p342-343, (1989)
References 2:
Parnes JR, Semin Immunol 6: 221-229 (1994)
References 3:
Delon J. et al. Immunity 9(4): 467-73 (1998)
References 4:
Akimoto H, et al. Immunology 95(2): 214-218 (1998)
Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of ?, ?, and ? chains. A5 reacts specifically with ? chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
A5
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ljubimov JY et. al. Cancer Res. 61(14): 5601-5610 (2001)
References 2:
Ljubimov AV et. al. Int J Cancer 50: 562-566 (1992)
Prostatic Specific Acid Phosphatase (PSAP) is a prostatic enzyme found in the glandular epithelium of the prostate. PSAP levels are elevated in hyperplastic prostate and prostate carcinoma, with the highest levels being detected in metastasized prostate cancer. Moderate overexpression of PSAP is also characteristic of diseases of the bone (such as Paget's disease or hyperparathyroidism), diseases of blood cells (such as sickle-cell disease), multiple myeloma, or lysosomal storage diseases (such as Gaucher's disease). PSAP is considered more sensitive, yet less specific, than PSA, however Anti-PSAP can act as a useful complement to Anti-PSA under suitable clinical contexts.
Peroxiredoxin-5 has a role in intracellular redox signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VKALNVEPDGTGLTC) corresponding to region (190-204 aa) of human Peroxiredoxin-5 conjugated to KLH. Human, mouse and rat sequences of this peptide are identical.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, IF, WB. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 2,000 is recommended. This antibody clearly detects a protein at approximately 22 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Peroxiredoxin-5 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
PAX5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX5 is useful in differentiating between classic Hodgkin's lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin's lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX5.
Parathyroid Hormone (PTH), also known as Parathormone or Parathyrin, is a hormone secreted by the parathyroid glands that functions to increase the concentration of calcium in the blood. Anti-Parathyroid Hormone (PTH) is useful for differentiating parathyroid hyperplasia/neoplasms from thyroid and metastatic neoplasms, and is also used in the consideration of parathyroid carcinomas located primarily in the anterior mediastinum.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC645
Antibody Isotype:
IgM
GMDN Code:
63169
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Parathyroid Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Rabbit anti-Nociceptin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
FUNCTION: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. PTM: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. SIMILARITY: Belongs to the opioid neuropeptide precursor family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Rat
Immunogen:
A synthetic peptide (C-TG ARKSARKLAN Q) as part of rat Nociceptin peptide (aa: 139-151) conjugated to diphtheria toxoid
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA. A dilution of 1: 1000 to 1: 3000 is recommended for both applications. This antibody may also be used for staining of nerve fibres in guinea pig myenteric plexus. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
There are no synonyms for this peptide; however the precursor protein contains: Neuropeptide 1; Nociceptin (Orphanin FQ; PPNOC; ORL1 receptor agonist); Neuropeptide 2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been tested by the use of different peptides including enkephalin, dynorphin and endorphin for absorption in immunihistochemistry. This antibody is known to cross react with guinea pig and rat.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1.28 mg/ml
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Azotobacter vinelandii (Gram-), Bradyrhizobium japonicum, Cyanobacteria, Cyanothece ATCC51142, Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Enterobacter , genera, euryachaeotes, Klebsiella pneumonia, Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp., Mesoorhizobium loti, Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, able to fix atmoshperic nitrogen, Rhizobium melilotiSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.In photobionts like Anabaena sp., low nitrate growth is required to turn on the NifH expression to high enough levels to detect NifH protein. Immunofluorescence protocol Insect dissected tissues (digestive tract, fat body, carrying NifH positive bacteria) of large workers were fixed in cold methanol (20 min, -20 °C) and then permeabilized in cold acetone (5 min, -20 °C). Samples were subsequently rinsed three times with PBS with 0.1 % Triton-X 100 at RT (PBST) and incubated for 5 minutes in PBST. This was followed by incubation of tissues for 1 hr with 6 ug/ml affinity purified anti-NifH antibody (Agrisera, AS01 021A) diluted in PBS-TBSA (PBS, 0.1 % v/v Triton-X-100, 1 mg/ml BSA) and 3 washings with PBST. Samples were then incubated in the dark with a goat anti-chicken IgY conjugated to Dylight 488 (Pierce, SA5-10070) for 45 min and were washed twice (PBS, 0.1%v/v Triton-X-100). Finally, the tissues were mounted in Vectashield medium containing DAPI (Vector Laboratories, H-1500) and viewed under a SP5 Leica confocal microscope with 10X and 63X objectives. Courtesy of Drs. Panagiotis Sapountzis and Mariya Zhukova, University of Copenhagen, Danmark
Application Details:
1 : 500 (IHC), 6 g/ml (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified IgY in PBS pH 8 and 0.02 % sodium azide.
Molecular Weight:
27 | 32.5 kDa
Not reactive in:
Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 as NifH protein is not present in those cyanobacterial species, Frankia sp.
Selected references:
Santana-Sanchez, et al. (2023) Flv3A facilitates O2 photoreduction and affects H2 photoproduction independently of Flv1A in diazotrophic Anabaena filaments. New Phytol. 2023;237(1):126-139. doi:10.1111/nph.18506Chen et al. (2022) Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system, Preprint from Research Square, 22 Jul 2022, DOI: 10.21203/rs.3.rs-1752770/v1Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151He et al. (2021) Vegetative cells may perform nitrogen fixation function under nitrogen deprivation in Anabaena sp. strain PCC 7120 based on genome-wide differential expression analysis. PLoS One. 2021 Mar 4;16(3):e0248155. doi: 10.1371/journal.pone.0248155. PMID: 33662009; PMCID: PMC7932525. (Immunolocalization)Liu et al. (2020). A VIT-like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean. New Phytol . 2020 Mar 2. doi: 10.1111/nph.16506.
MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75 NTR , the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75 NTR , saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75 NTR -expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo , MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75 NTR receptors.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat NGF receptor (p75NTR)
Applications:
In-vivo
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
1. To specifically target and eliminate rat cells expressing p75NTR <i>in vivo</i>. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease. <br> 2. To be used as a model for gene delivery into neurons.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Active. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
Mouse anti-Nestin Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240 kDa. However the real molecular weight is considerably less than this, at 177 kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial segment (region 317-630 aa) of human Nestin expressed in E.coli
Applications:
ICC,WB
Clone number:
4D11
Antibody Isotype:
IgG1, kappa
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. Suggested dilution for WB is 1:1,000-5,000 and 1:250-500 for IC. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Nestin; NES;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Schomann T et al. (2020) Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury. Cell Tissue Res. [Epub ahead of print]. Application: IHC/IF; Species: Mouse. Schomann T et al. (2017) Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants. PLos One. 12(10):e0187183. Application: ICC/IF; Species: Mouse, Hair follicle bulge-derived neural crest-derived stem cells (HFBSCs). Gho CG et al. (2015) Isolation, expansion and neural differentiation of stem cells from human plucked hair- a further step towards autologous nerve recovery. Cytotechnology In press. Application: IF; Species: Human, Hair follicle bulge-derived neural crest-derived stem cells (HFBSCs), Keywords: Hair follicle stem cell, Regeneration, Neural crest, Neuron, Glia, Cryopreservation
Specificity:
This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band. Human, Rodent
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
The Nestin [IHC105] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC105
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Glioma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
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