Background Info: |
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria, The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II, PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts, Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples, Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1 |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Liquid in PBS pH 7,4. |
Storage Temp: |
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use. |
Host Animal: |
Rabbit |
Species Reactivity: |
Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp,, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp,, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp,, Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrella patens, Synechococcus sp, PCC 7942, Paulinella chromatophora (amoeba), Prochlorococcus sp, (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp,, Zea mays |
Expected Species: |
Algae (brown and red), Dictos, Conifers, Cyanobacteria, Medicago sativa, Nannochloropsis sp., Pisum sativum, Triticum aestivum, cellular [compartment marker] of thylakoid membrane.Species of your interest not listed? Contact us |
Immunogen: |
Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp,, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp,, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp,, Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrella patens, Synechococcus sp, PCC 7942, Paulinella chromatophora (amoeba), Prochlorococcus sp, (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp,, Zea mays |
Applications: |
Immunofluorescence (IF), Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions, In our analysis we have seen both, ca, 24 kDa and ca, 10 kDa fragments from different samples, depending on treatments and isolation procedures,Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e,g, precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016,This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel,The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43, |
Application Details: |
To be determined by end user |
Purity: |
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650. |
Molecular Weight: |
38 | 28-30 kDa |
Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known, |
Selected references: |
To be added when available. Antibody released in May 2023. |
Special application note: |
Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands,Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries. |
UniProt number: |
P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660 |
TAIR number: |
ATCG00020 |
Research area: |
Global Antibodies |
Code: |
Primconj10 |
|
Background Info: |
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp. (roots, leafs, pollen), Nicotiana bentamiana, Nicotiana tabacum, Populus sp., Spinacia oleracea, Zea mays. Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria |
Expected Species: |
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3 |
Applications: |
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 g/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus. |
Application Details: |
1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB) |
Purity: |
Serum |
Reconstitution: |
For reconstitution add 10 µl of sterile water |
Molecular Weight: |
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea) |
Not reactive in: |
Archeal V-type ATP synthase |
Selected references: |
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30. |
Special application note: |
This product can be sold containing proClin if requested.The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein. |
UniProt number: |
P19366 , P83483 , P06541 , A8IQU3 |
TAIR number: |
AT5G08670, ATCG00480 |
Research area: |
Global Antibodies |
Code: |
Photo20 |
|