PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 g/ l total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication).
Product Type:
Antibody
Storage Temp:
Stable at RT for at least 1 month; short-term storage (6 monthss) at 4°Cand long term storage (1 year or more) at -20 °C.
Species Reactivity:
PEB has been tested on a wide range of species and tissues from Higher plants, Mosses, Llichens, Algae, Diatoms, Dinoflagellates, Cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western blotting, and Immunoprecipitation (IP). Most of Agrisera commercial antibodies are tested on plant or algal samples extracted with this buffer. An example can be found here.
5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)
Selected references:
Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w.P rez-L pez et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784.Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom)Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80.Matsuo and Atsumi (2018). Xylosylation of proteins by expression of human xylosyltransferase 2 in plants. J Biosci Bioeng. 2018 Sep;126(3):371-378. doi: 10.1016/j.jbiosc.2018.03.013.Brouwer et al. (2011) TheImpact ofLightIntensity onShade-InducedLeaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).Kosawang et al. (2011) Hydrogen yield from a hydrogenase in Frankia R43 at different levels of the carbon source propionate. Journal of Environmental Management, Jan 26
Special application note:
Buffer components (4x): contains ~ 40% v/v glycerol [HOCH2CH(OH)CH2OH], Tris-HCl [NH2C(CH2OH)3 HCl] pH 8.5, LDS [CH3(CH2)11OSO3Li], EDTA [(HO2CCH2)2NCH2CH2N(CH2CO2H)2]It is recommended to include a protease inhibitor (not supplied with this buffer) from a freshly made stock while preparing the ready-to-use 1x PSB.PEB has been optimized for quantitative small-scale preparation of whole protein extracts from plant/algal tissue. Extraction using the procedure described below will result in maximum yield of proteins and diminish protein degradation and aggregation.Extracts may be quantified using detergent (LDS) compatible methods and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western Blotting, and immunoprecipitation.PEB has been tested on a wide range of species and tissues from higher plants, mosses, lichens, algae, diatoms, dinoflagellates, and cyanobacteria.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on mouse IgG and light chains on all mouse immunoglobulins
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Not reactive in:
Non-immunoglobulin mouse serum proteins
Selected references:
Kasari et al. (2019). A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling. Nucleic Acids Res. 2019 Jul 12. pii: gkz600. doi: 10.1093/nar/gkz600.Li and Bock (2018). Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum. Nat Commun. 2018 Aug 27;9(1):3451. doi: 10.1038/s41467-018-05651-1.Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
Special application note:
Concentration: 1.0 mg/ml. Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) ProClin 150 as preservative of bacterial growth.The antibody will detect all isotypes of mouse IgG.
Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum, Synechocystis PCC 6803
Expected Species:
Red Algae, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
Special application note:
This product can be sold containing proclin if requested
Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence, UniProt:Q9FEK6
For optimal detection, load/well should be from 5 to 10 g of total protein. Higher protein load combined with weak blocking will contribute to detection of other bands.
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
30 | 26 kDa
Not reactive in:
Other algae
Selected references:
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Drop et. al (2014). Consequences of state transitions on the structural and functional organization of Photosystem I in the green alga Chlamydomonas reinhardtii. Plant J. 2014 Feb 8. doi: 10.1111/tpj.12459.
Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Nicotiana tabacum, Zea maysSpecies of your interest not listed? Contact us
Nishioka et al. (2021). Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane. Photosynth Res. 2021 Jan;147(1):107-124. doi: 10.1007/s11120-020-00803-1. Epub 2020 Dec 2. PMID: 33269435; PMCID: PMC7728655.Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.
LhcSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Physcomitrium patens
Expected Species:
Chlorella variabilis, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
Recombinant LHCSR of Physcomitrella patens, overexpressed in E.coli, UniProt: A9TED6 and Pp1s213_80V6 (phytozome)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Furukawa et al. (2019). Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens. J Plant Res https://doi.org/10.1007/s10265-019-01138-2.Pinnola at al. (2015). Light-Harvesting Complex Stress-Related Proteins Catalyze Excess Energy Dissipation in Both Photosystems of Physcomitrella patens. Plant Cell. 2015 Nov;27(11):3213-27. doi: 10.1105/tpc.15.00443. Epub 2015 Oct 27.
Special application note:
This product can be sold contiaing ProClin if requested.
LHCSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody is also recognizing recombinant LHCSR1 overexpressed in E.coli as described in Perozeni et al. (2020).
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
27 kDa
Not reactive in:
Lobosphaera incisa, Phaeodactylum tricornutum
Selected references:
Cazzaniga et al. (2022). Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii. Biotechnol Biofuels Bioprod. 2022 Jul 11;15(1):77. doi: 10.1186/s13068-022-02173-3. PMID: 35820961; PMCID: PMC9277849.Redekop et al. (2020). PsbS Contributes to Photoprotection in Chlamydomonas Reinhardtii Independently of Energy Dissipation . Biochim Biophys Acta Bioenerg . 2020 Jun 1;1861(5-6):148183.doi: 10.1016Lammermann et al. (2020). Ubiquitin ligase component LRS1 and transcription factor CrHy5 act as a light switch for photoprotection in Chlamydomonas. doi.org/10.1101/2020.02.10.942334 bioRxivRoach et al. (2020). The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii. J Exp Bot. 2020 Jan 16. pii: eraa022. doi: 10.1093/jxb/eraa022.Gabilly et al. (2019). Regulation of photoprotection gene expression in Chlamydomonas by a putative E3 ubiquitin ligase complex and a homolog of CONSTANS. Proc Natl Acad Sci U S A. 2019 Aug 12. pii: 201821689. doi: 10.1073/pnas.1821689116.
Tic40 is a component of the inner envelope membrane import complex (TIC) of plant chloroplasts. Tic40 has been proposed to function as a co-chaperone in the stromal chaperone complex that facilitates protein translocation across the inner membrane. Tic40 can be used as a cellular [compartment marker] for chloroplast inner envelope membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
CHLH (GUN5) (Magnesium-chelatase subunit ChlH, chloroplastic) is a protein involved in chlorophyll synthesis, plastid to nucleus retrograde signaling and ABA perception. Alternative names: ABA-binding protein, Mg-protoporphyrin IX chelatase subunit ChlH, Protein CONDITIONAL CHLORINA, Protein GENOMES UNCOUPLED 5, Protein RAPID TRANSPIRATION IN DETACHED LEAVES 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Acaryochloris marina, Halomicronema hongdechloris, Synechocystis sp. 6803Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana CHLH protein sequence, UniProt: Q9FNB0, TAIR: At5g13630
Lhcbm5 is one of minor LHCII that associates with PSI in state-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
For western blot detection image please refer to the article below
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
29 | 30 kDa
Selected references:
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Pinnola (2021). The rise and fall of Light-Harvesting Complex Stress-Related proteins as photoprotection agents during evolution. J Exp Bot. 2019 Oct 24;70(20):5527-5535. doi: 10.1093/jxb/erz317. PMID: 31424076.Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457.
Special application note:
This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000This product can be sold containing ProClin in requested.
Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 g/liter of culture.Synonyme: HYD1/1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
53,7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin‑hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657–3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
In Chlamydomonas HydA is present in low levels of 1 g/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 g of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 g of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.
Special application note:
Protein name was changed from APG7 to ATG7 after Doelling paper was published.
Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Solanum lycopersicum, Nicotiana tabacum Species of your interest not listed? Contact us
Immunogen:
Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880
This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
61 | 54 kDa
Not reactive in:
Pinus strobus
Selected references:
Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Kim et al. (2013). Mutation of the Arabidopsis NAC016 Transcription Factor Delays Leaf Senescence.'Plant Cell Physiol. Aug 21.Nagane et al. (2010). Involvement of AtNAP1 in thre reulation of chlorophyll degradation in Arabiopsis thaliana. Planta (4):939-949.Hirashima et al. (2009). Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana. Plant Cell Physiol. (4):719-729.
Special application note:
The protein level is moderately induced during dark-induced senescence
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae (brown and red), Conifers, Cyanobacteria, Brassica napus, Diatoms, Glycine max, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel
Application Details:
1 : 1000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
38 | 28-30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..G recka et al. (2019). Photosystem II 22kDa protein level a prerequisite for excess light-inducible memory, cross-tolerance to UV-C, and regulation of electrical signalling. Plant Cell Environ. 2019 Nov 23. doi: 10.1111/pce.13686.Liu et al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080.
Special application note:
Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.This product can be sold containing ProClin if requested.
LHCR4 (Protein fucoxanthin chlorophyll a/c protein) is a PSI supercomplex peripheral antenna protein, identified by proteome analysis as type‐R light‐harvesting complexes (LHCr4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Phaeodactylum tricornutum
Expected Species:
Gambierdiscus australes, Madurella mycetomatisSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Phaeodactylum tricornutum LHCR4, UniProt: B7FQE1
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested
Application Details:
1 : 1000 (BN-PAGE), (WB)
Purity:
Antigen affinity purified in PBS pH 7.4
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
82,7 | 55-60 kDa
Not reactive in:
Chlamydomonas reinhardtii, dinoflagellate
Selected references:
Shukla et al. (2020). A novel method produces native LHCII aggregates from the photosynthetic membrane revealing their role in non-photochemical quenching. J Biol Chem. 2020 Oct 20:jbc.RA120.016181. doi: 10.1074/jbc.RA120.016181. Epub ahead of print. PMID: 33082138.Grieco et al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756. Liu et al. (2020). Acid treatment combined with high light leads to increased removal efficiency of Ulva prolifera. Algal Research,Volume 45, January 2020, 101745Frede et al. (2019). Light quality-induced changes of carotenoid composition in pak choi Brassica rapa ssp. chinensis. J Photochem Photobiol B. 2019 Apr;193:18-30. doi: 10.1016/j.jphotobiol.2019.02.001.Lima-Melo et al. (2019). Consequences of photosystem-I damage and repair on photosynthesis and carbon use in Arabidopsis thaliana. Plant J. 2018 Nov 29. doi: 10.1111/tpj.14177.
The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
Loads higher than 0,5 g of chlorophyll per well are not recommended
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
4.3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dobakova et. al (2007). Role of the PsbI Protein inPhotosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.
GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Agrawal et al. (2020). The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiol. 2020 Feb 18. pii: pp.00009.2020. doi: 10.1104/pp.20.00009Montandon et al. (2019). In vivo trapping of proteins interacting with the chloroplast CLPC1 chaperone; potential substrates and adaptors. J Proteome Res. 2019 May 9. doi: 10.1021/acs.jproteome.9b00112.Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
Special application note:
Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).
G4 (Chlorophyll synthase) is an enzyme which performs estrification of chlorophyllide (a and b), which is the last step of chlorophyll biosynthesis. Alternative names: polyprenyl transferase, protein G4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Avena sativa, Camelia sinensis, Cocomyxa subellipsoidea, Gossypium hirsutum, Micromonas pusilla, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Ricinus communis, Triticum urartu, cyanobacteriaSpecies of your interest not listed? Contact us
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c?-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Acacia prainii, Angelphytum bahiense, Aristolochia promissa, Chlamydomonas reinhardii, Daucus glochidiatus, Dimerostemma brasilianum, Elaphandra paucipunctata, Fraxinus chiisanensis, Gossypium populifolium, Hydrangea heteromalla, Nicotiana sylvestris, Oryza sativa, Salix tetrasperma, Solanum lycopersicum, Thottea siliquosa, Triticum turgidum, Ulva flexuosa, Wedelia biflora, Vanilla aphyllaSpecies of your interest is not listed? Species of your interest not listed? Contact us. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from Arabidopsis thaliana PetB protein sequence, UniProt: P56773, TAIR: ATCG00720
Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105027Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Lu et al. (2021). Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12(1):679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763.Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.
The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence. Synonymes: NPQ4 (NONPHOTOCHEMICAL QUENCHING).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Chlamydomonas reinhardtii, Cucumis sativus, Medicago truncatula, Physcomitrium patens, Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, Solanum lycopersicum, Tarenaya hassleriana, Zosteria marina, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide located in solubilized part of the protein, derived from available di- and monocot PsbS sequences, including Arabidopsis thaliana UniProt:Q9XF91, TAIR:At1g44575
This product can be sold containing proclin if requested
Application Details:
1 : 2000 - 1: 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
28 | 22 kDa for Arabidopsis thaliana
Not reactive in:
Lobosphaera incisa
Selected references:
Jiang et al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399. Barbato et al. (2020). Higher Order Photoprotection Mutants Reveal the Importance of ?pH-dependent Photosynthesis-Control in Preventing Light Induced Damage to Both Photosystem II and Photosystem I. Sci Rep . 2020 Apr 21;10(1):6770. doi: 10.1038/s41598-020-62717-1.Nikkanen et al. (2018). Multilevel regulation of non-photochemical quenching and state transitions by chloroplast NADPH-dependent thioredoxin reductase. Physiol Plant. 2018 Dec 22. doi: 10.1111/ppl.12914.Chen et al. (2018). Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity. Physiol Plant. 2018 Apr 6. doi: 10.1111/ppl.12737.Glowacka et al. (2018). Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop. Nat Commun. 2018 Mar 6;9(1):868. doi: 10.1038/s41467-018-03231-x.
LHCSR3 (Stress-related chlorophyll a/b binding protein 3) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Bohmer et al. (2023) Chlamydomonas reinhardtii mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress. Plant Direct. 2023;7(1):e480. Published 2023 Jan 15. doi:10.1002/pld3.480Cazzaniga et al. (2022). Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii. Biotechnol Biofuels Bioprod. 2022 Jul 11;15(1):77. doi: 10.1186/s13068-022-02173-3. PMID: 35820961; PMCID: PMC9277849.Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Roach et al. (2020). The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii. J Exp Bot. 2020 Jan 16. pii: eraa022. doi: 10.1093/jxb/eraa022.
PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II. Synonym: Photosystem I P700 chlorophyll a apoprotein A1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Immunogold localization has been done in leaf material of Arabidopsis thaliana.
Application Details:
1 : 20 (IG), 1 : 1000-1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
82 | 55-60 kDa
Not reactive in:
Chromera velia
Selected references:
Ivanov et al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Lim et al (2022) Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Spaniol et al. (2022) Complexome profiling on the Chlamydomonas lpa2 mutant reveals insights into PSII biogenesis and new PSII associated proteins, Journal of Experimental Botany, Volume 73, Issue 1, 5 January 2022, Pages 245–262Rogowski et al. (2021) Light as a substrate: migration of LHCII antennas in extended Michaelis-Menten model for PSI kinetics. J Photochem Photobiol B. 2021 Dec;225:112336. doi: 10.1016/j.jphotobiol.2021.112336. Epub 2021 Oct 19. PMID: 34736069.
Special application note:
PsaA is a hydrophobic protein and we recommend to use PVDF membrane for transfer to assure best results.This product can be sold containing ProClin if requested.
VDE (violaxanthin de-epoxidase) is a part of the xanthophyll (or violaxanthin) cycle for controlling the concentration of zeaxanthin in chloroplasts. This enzyme is catalyzing the two-step mono de-epoxidation reaction. Zeaxanthin induces the dissipation of excitation energy in the chlorophyll of the light-harvesting protein complex of photosystem II.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
PsbTn (Tn protein of PSII) nuclear encoded protein involved in photosynthesis. Alternative names: photosystem II 5 kDa protein, chloroplastic, PSII-T, Nuclear encoded psbT.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Cicer arietinum, Glycine soja, Helianthus annuus, Medicago truncatula, Nicotiana attenuata, Nicotiana tabacum, Nicotiana sylvestris, Noccaea caerulescens, Petunia hybrida, Populus trichocarpa, Solanum chacoense, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana PsbTn protein sequence, UniProt:Q39195, TAIR: AT3G21055
LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel.Protocol: Add acetone to final concentration of 80% ice-cold acetone. Leave 10 minutes. Spin. Rresuspend pellet in solubilisation buffer and load on a gel.
Application Details:
1: 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
11 | 5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chen et al. (2019). The Low Molecular Mass Photosystem II Protein PsbTn is Important for Light Acclimation. Plant Physiol. Apr;179(4):1739-1753. doi: 10.1104/pp.18.01251.
UniProt number:
Q39195
TAIR number:
AT3G21055
Research area:
PSII (Photosystem II)
Code:
Photo20
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