| Background Info: |
CesA4 (IRX5) (EC=2.4.1.12) is a catalytic subunit of xylem-specific cellulose synthase enzyme, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA7 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites. Expressed in young plants, stems and flowers but not in leaves, roots and shoots. Amounts are increasing as stems mature. Synonymes: Protein IRREGULAR XYLEM 5, IRX5, NWS2. |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Solanum lycopersicum, Solanum tuberosum |
| Expected Species: |
Betula luminifera, Brassica napus, Capsella rubella, Eutrema salsugineum, Gossypium hirsutum, Nelumbo nucifera, Noccaea caerulescens, Vitis viniferaSpecies of your interest not listed? Contact us |
| Immunogen: |
Recombinant Arabidopsis thaliana IRX5 fragment, UniProt: Q84JA6,TAIR: At5g44030 |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
Oryza sativa |
| Application Details: |
1 : 1000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 50 l of sterile water |
| Molecular Weight: |
119,5 kDa |
| Not reactive in: |
Populus tremula |
| Selected references: |
Otulak-Kozieł et al. (2018). Plant Cell Wall Dynamics in Compatible and Incompatible Potato Response to Infection Caused by Potato Virus Y (PVYNTN). Int J Mol Sci. 2018 Mar 15;19(3). pii: E862. doi: 10.3390/ijms19030862.Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323. |
| Special application note: |
This antibody is detecting both, recombinant and edogenous CesA4 (IRX5) protein |
| UniProt number: |
Q84JA6 |
| TAIR number: |
AT5G44030 |
| Research area: |
Cell wall marker |
| Code: |
CM20 |
|
| Product Detail: |
CGRP (Calcitonin Gene Related Peptide) Antibody |
| Background Info: |
Raised to rat alpha-CGRP coupled to bovine thyroglobulin with glutaraldehyde. |
| Product Type: |
Antibody - Antibodies |
| Antibody Type: |
polyclonal |
| Format: |
Lyophilised |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Mouse, Rat, Salmon, Turtle |
| Immunogen: |
rat alpha-CGRP |
| Applications: |
Immunohistochemistry, Immunocytochemistry, immunofluorescence |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
The CGRP Antibody was raised to rat alpha-CGRP coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven fluorescein staining at a 1/100-1/200 dilution and a strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat -CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y. |
| Gene Symbol: |
Calca |
| NCBI Gene Aliases: |
CAL6,CGRP,Cal1,Calc,RATCAL6,calcitoni |
| PubMed IDs: |
3711894, 2794128, 1981998, 1699158, 1587996, 1479068, 1385495, 1384974, 24732837, 25326085 |
| Product category: |
Cancer, Hypoxia, Pain, Stem Cell, Transcription Factor, |
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