Product Detail: |
Mouse anti-CD105/Endoglin, clone E9 (Monoclonal) |
Antibody Type: |
Monoclonal |
Host Animal: |
Mouse |
Species Reactivity: |
human |
Immunogen: |
Unknown or proprietery to MONOSAN and/or its suppliers |
Applications: |
IHC-fr,FC,ELISA,IP,WB |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
The monoclonal antibody E9 reacts with Endoglin, a 190 kDa homodimeric transmembrane glycoprotein composed of disulfide-linked subunits. The external domain binds TGF-beta1 and -beta3 isoforms with high affinity. Two different isoforms (L and S) of CD105 with capacity to bind TGF-beta have been characterized, which differ in the amino acid composition of their cytoplasmic tails. Mutations in the gene encoding endoglin have been linked to the human disease hereditary hemorrhagic telangiectasia type 1 (HHT1), a vascular disorder characterized by localized vascular dysplasia and a tendency towards arteriovenous malformations. Mice expressing a single CD105 allele develop external signs of disease similar to human HHT1, supporting the haploinsufficiency model for HHT1. Mice lacking endoglin die from defective angiogenesis characterized by failure of vascular smooth muscle investment of embryonic blood vessels, suggesting a defect in vascular smooth muscle cell development. Microvessel density (MVD) has been reported to be an independent prognostic indicator of outcome in a variety of human malignancies, with increased MVD correlating with shorter overall and relapse-free survival rates. The MVD counts using anti-CD105 antibody significantly correlated with overall and disease-free survival. Anti-CD105 monoclonal antibody E9 and anti-CD34 monoclonal antibody have been successfully used to quantify MVD in human breast carcinoma. The monoclonal antibody E9, directed against CD105, has also been used as a prognostic marker for primary central nervous system lymphomas. |
Antibody Isotype: |
IgG1 |
Monosan Range: |
MONOSAN |
Clone: |
E9 |
Concentration: |
100 ug/ ml |
Storage buffer: |
PBS with 0.1% BSA and 0.02% sodium azide |
Storage: |
2-8°C |
References 1: |
Wang; J et al. J Natl Cancer Inst 1994; 86: 386 |
References 2: |
Pichuantes, S et al Tissue Antigens 1997, 50: 265 |
References 3: |
Kumar; S et al. Cancer Research 1999; 59: 856 |
References 4: |
Li C et al. Int J Cancer 2000; 89: 122 |
References 5: |
Li C et al. B J Cancer 2003; 88: 1424 |
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Product Detail: |
Mouse ENTPD2 ELISA Kit (96 Tests). Quantitate Mouse Entpd2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. |
Product Type: |
Assay & Detection |
Storage Temp: |
Mixed |
Immunogen: |
Expression system for standard: NS0; Immunogen sequence: C26-S462 |
Applications: |
ELISA |
Additional Info: |
For quantitative detection of mouse ENTPD2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Biosite Brand: |
BioSite ELISA |
Species Reactivity: |
mouse |
Cross Reactivity: |
There is no detectable cross-reactivity with other relevant proteins. |
EntrezGene ID: |
12496 |
UniProt No: |
O55026 |
|
Background Info: |
PGR5 (Protn gradient regulation 5) is involved in the regulation of the cyclic electron flow (CEF) around Photosystem I and cellular response to light intensity and photoprotection. Essential for the reduction of PGRL1A by ferredoxin and for photoprotection. |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidopsis thaliana, Picea abies, Pinus sylvestris, Solanum lycopersicum |
Expected Species: |
Amaranthus hybridus, California macrophylla, Cucumis melo, Cucumis sativus, Erodium gruinum, Francoa sonchifolia, Geranium phaeum, Gossypium hirsutum, Helianthus annuus, Hypseocharis bilobata, Juglans regia, Klebsormidium nitens, Medicago truncatula, Melianthus villosus, Monsonia marlothii, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Noccaea caerulescens, Pelargonium dichondrifolium, Physcomitrium patens, Pinus taeda, Populus sp. , Portulaca oleracea, Spinacia oleracea, Theobroma cacao, Trifolium pratense, Zea mays, Zostera marina, Vitis viniferaSpecies of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated peptide derived from Arabidopsis thaliana PGR5, UniProt: Q9SL05, TAIR: AT2G05620 |
Applications: |
Immunoprecipitation (IP), Western blot (WB) |
Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
Additional Info: |
This product can be sold containing proclin if requested |
Application Details: |
1: 1000 - 1: 5000 (WB) |
Purity: |
Serum |
Reconstitution: |
For reconstitution add 50 l of sterile water |
Molecular Weight: |
14,29 kDa |
Not reactive in: |
diatoms, Chlorella sp., cyanobacteria |
Selected references: |
Cao et al. (2022) Autophagic pathway contributes to low-nitrogen tolerance by optimizing nitrogen uptake and utilization in tomato. Hortic Res. 2022 Mar 23;9:uhac068. doi: 10.1093/hr/uhac068. PMID: 35669705; PMCID: PMC9164271.Ermakova et al. (2022) Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot. 2022 Jul 29:erac320. doi: 10.1093/jxb/erac320. Epub ahead of print. PMID: 35904136.Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105026Yang et al. (2020). Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring. Nat Commun. 2020 Jan 8;11(1):128. doi: 10.1038/s41467-019-13954-0.Rantala et al. (2020). PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861(3):148154. doi: 10.1016/j.bbabio.2020.148154. |
UniProt number: |
Q9SL05 |
Research area: |
Electron transfer |
Code: |
Photo20 |
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