The parathyroid glands function within the endocrine system to promote blood calcium homeostasis through controlled release of parathyroid hormone (PTH). This process involves the synthesis and secretion of PTH by activated parathyroid chief cells during conditions of hypocalcemia. With the anatomical proximity to the thyroid and capacity of associated neoplasms of the parathyroid to mimic thyroid tumors, challenges can arise in distinguishing between these types of abnormalities. In cases where there is uncertainty about a tumor being of parathyroid origin, immunohistochemical evaluation using anti-PTH can be of value.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN Ready To Use
Clone:
MRQ-31
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Aldinger KA, et al. Cancer; 49:388-97 (1982)
References 2:
Brown EM. Mineral Electrolyte Metal; 8:130-50 (1982)
References 3:
Abate EG, et al. Front Endocrinol (Lausanne).; 7:172 (2017)
References 4:
Duan K, et al. Turk Patoloji Derg.; 31 Suppl 1:80-97 (2015)
References 5:
Chen HL, et al. Journal of Biology and Chemistry; 277:19374-81 (2002)
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkins disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
IPO-38
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Sidorenko, S.P. et al., Gematol. Transfuziol. 35: 19-22 (1990)
References 2:
Sidorenko, S.P. et al., Experem. Oncol. 16: 145-150 (1994)
References 3:
Thosaporn W., et al., Oral Dis. 10(1): 22-6 (2004)
References 4:
Hao Y. et al.,J Proteome Res. Sep;7(9): 3668-77 (2008)
References 5:
Makohon N.V. et al., Fiziol Zh. 54(6): 49-57 (2008)
MoBU-1 is reactive with cells containing incorporated 5-bromodeoxyuridine (BrdU) showing a clear, nucleus confined, speckled pattern. BrdU is an analogue of thymidine and can be introduced to proliferating cells in vitro or in vivo, which in turn incorporate BrdU into the DNA during S phase, prior to cell division. Immunocytochemical staining with MoBU-1 will show the degree of proliferation, detecting nucleated cells which have incorporated BrdU in place of thymidine into their DNA.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
MoBU-1 (85-2C8)
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988)
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an ? and ? chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B-cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-O-111
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Thompson CJ et al., Human Immunol 6: 133-150 (1983)
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
47-8D3
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Flavell DJ. et al., J. Histochem. Cytochem. 35: 1217-1226 (1987)
References 2:
Facchetti F. et al., Am. J. Clin. Pathol. 92: 42-50 (1989)
References 3:
Bardadin KA. et al., J. Pathol. 164: 253-259 (1991)
References 4:
Goebeler M. et al., J. Leukocyte Biol. 55: 259-261 (1994)
RAb-50 reacts with SAD-Vnukovo and Pitman-Moore strains of rabies virus. It shows conformation dependent specificity for the viral envelope glycoprotein. In immunofluorescence test, SAD-Vnukovo strain is recognized by the antibody, while in Western blot, both strains are recognized by the antibody. In ELISA only SAD-Vnukovo strain is recognized. RAb-50 can neutralize the CVS strain of rabies virus.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
RAB-50
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Macikova, I. et al, Acta virol. 36: 541-550 (1992)
References 2:
Sajjanar B, et al, Neuropeptides. 57: 59-64 (2016)
References 3:
Chen Li, et al, Acta Pharmacol Sin. 32(3): 329337 (2011)
T-cell leukemia/lymphoma protein 1A (TCL1) is a member of theTCL1 family and enhances the phosphorylation and activation ofAKT1, AKT2 and AKT3. TCL1promotes the nuclear translocation ofAKT1 and enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. The expression of TCL1 is restricted to lymphoid cells. It is expressed early in lymphocyte differentiation. Strong expression ofTCL1 is found in a subset of mantle zone B lymphocytes and is expressed to a lesser extent by follicle center cells. In B cell neoplasia, TCL1 immunoreactivity is found in the majority of B cell lymphomas including lymphoblastic lymphoma, chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma (60%), and primary cutaneous B cell lymphoma (55%). The expression of theTCL1genecharacterizes low-grade B cell lymphomas.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP105
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Laine J, et al. Mol Cell2000, 6:395-407
References 2:
Pekarsky Y, et al. Proc Natl Acad Sci U S A, 2000, 97:3028-3033
References 3:
Narducci MG, et al. Cancer Res, 2000, 60:2095-2100
The parathyroid glands function within the endocrine system to promote blood calcium homeostasis through controlled release of parathyroid hormone (PTH). This process involves the synthesis and secretion of PTH by activated parathyroid chief cells during conditions of hypocalcemia. With the anatomical proximity to the thyroid and capacity of associated neoplasms of the parathyroid to mimic thyroid tumors, challenges can arise in distinguishing between these types of abnormalities. In cases where there is uncertainty about a tumor being of parathyroid origin, immunohistochemical evaluation using anti-PTH can be of value.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRQ-31
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Aldinger KA, et al. Cancer; 49:388-97 (1982)
References 2:
Brown EM. Mineral Electrolyte Metal; 8:130-50 (1982)
References 3:
Abate EG, et al. Front Endocrinol (Lausanne).; 7:172 (2017)
References 4:
Duan K, et al. Turk Patoloji Derg.; 31 Suppl 1:80-97 (2015)
References 5:
Chen HL, et al. Journal of Biology and Chemistry; 277:19374-81 (2002)
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3GF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-). Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.
Application Details:
1 : 20 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
37,5 | 25,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.
LhcSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Physcomitrium patens
Expected Species:
Chlorella variabilis, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
Recombinant LHCSR of Physcomitrella patens, overexpressed in E.coli, UniProt: A9TED6 and Pp1s213_80V6 (phytozome)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Furukawa et al. (2019). Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens. J Plant Res https://doi.org/10.1007/s10265-019-01138-2.Pinnola at al. (2015). Light-Harvesting Complex Stress-Related Proteins Catalyze Excess Energy Dissipation in Both Photosystems of Physcomitrella patens. Plant Cell. 2015 Nov;27(11):3213-27. doi: 10.1105/tpc.15.00443. Epub 2015 Oct 27.
Special application note:
This product can be sold contiaing ProClin if requested.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-M), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
synthetic peptide chosen from human Abeta protein (3-10) pregion, oligomer specific
Applications:
Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Meilandt et al. (2019). Characterization of the selective in vitro and in vivo binding properties of crenezumab to oligomeric A ?². Alzheimers Res Ther. 2019 Dec 1;11(1):97. doi: 10.1186/s13195-019-0553-5.Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
Special application note:
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This antibody is specific for human Amyloid-Beta oligomers.
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles, It consists of three clathrin heavy chains and three light chains, Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)
Application Details:
1: 100 (IL), 1 : 5000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
12.5 | 14 kDa (for Arabidopsis thaliana)
Not reactive in:
Arabidopsis thaliana CytC6, Chinesecuscuta sp.
Selected references:
Guo et al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana,Biochemical and Biophysical Research Communications,Volume 578,2021,Pages 63-69,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2021.09.022.(https://www.sciencedirect.com/science/article/pii/S0006291X21013164)Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Dai et al. (2020). Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25;eraa348.doi: 10.1093/jxb/eraa348. Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Doronina et al. (2019). Structural and Functional Features of the Wheat Embryo Sac’s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194–208. (immunolocalization)
UniProt number:
D7KMK0 , D7LY03
TAIR number:
AT1G22840, At4g10040
Research area:
Mitochondria | Respiration
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