> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Accession Number:
AAA51107
Trademark:
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · serum proteins from bovine, horse, human rabbit or swine
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.8, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Preservative:
0.1% (v/v) Kathon CG
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
Store freeze-dried powder at 2-8 °C.
Shelf Life:
Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Host:
Goat
Immunogen:
Purified Mouse IgG, whole molecule
Buffer:
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins · human IgG or serum proteins
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
The PD-L1-TEC [IHC451] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Applications:
IHC
Clone number:
IHC451
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Lung Adenocarcinoma
Buffer:
Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
Progesterone Receptor (PR), also known as NR3C3 (Nuclear Receptor Subfamily 3, Group C, Member 3), is an intracellular steroid receptor which mediates the physiological effects of progesterone, a female sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis. Progesterone receptor expression has been linked to the prediction of prognosis in breast cancer, as well as associated responses to endocrine therapy. The progesterone receptor has also been linked to risk for ovarian cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC751
Antibody Isotype:
IgG1, kappa
GMDN Code:
57534
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast, Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Specificity:
Based on IEP, this antibody reacts with: · heavy (γ) chains on mouse IgG · light chains on all mouse immunoglobulins
Cross Reactivity:
Based on IEP, no reactivity is observed to: · non-immunoglobulin mouse serum immunoglobulins
Country Of Origin:
Llama serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.SpecificityThe mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.Application detailsFlow cytometry: Recommended dilution: 1-4 µg/ml
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
15-414
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90% identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain.SpecificityThe mouse monoclonal antibody B4B6 recognizes an extracellular epitope of CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.Application detailsFlow cytometry: Recommended dilution: 1-4 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
SE5A5
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.SpecificityThe mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.Application detailsWestern blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 ?g/ml.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
A67-B/E3
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.SpecificityThe mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.Application detailsWestern blotting: Recommended dilution: 1 ?g/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
GCP-04
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate. The antibody should be used on formalin fixed paraffin embedded tissue sections, it is not advisable to use the antibody on frozen sections.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
NKI/C3
Concentration:
n/a
Storage buffer:
50 mM PBS at pH 7.4 with 1% BSA and 15mM sodium azide
Storage:
2-8°C
References 1:
MacKie, R.M., et al., J. Clin. Pathol. 37, 367 (1984)
References 2:
van Duinen, S.G., et al., Cancer 53, 1566 (1984)
References 3:
Vennegoor, C., et al., Int. J. Cancer 35, 287 (1985)
References 4:
Palmer, A.A., et al., Pathology 17, 335 (1985)
References 5:
Hagen, E.C., et al., Histopathology 10, 689 (1986)
C-51 reacts with keratin 8 (52.5 kDa) + 18 (45 kDa) polypeptides and recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-51
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. Pathol. 164: 215-24 (1991)
References 2:
Bártková et al. Neoplasma 38(4): 439-46 (1991)
References 3:
Wendl, J. et al. Anat. Histol Embryol. 164: 215-24 (1991)
References 4:
Thangappan, R et al. Cell. Polif. 42: 770-779 (2009)
EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.
Antibody Isotype:
IgG2a-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-003
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi D. et al, J. Pathol. 159: 7-12 (1989)
References 2:
Ivanyi D. et al, Am. J. Vet. Res. 53: 304-314 (1992)
References 3:
Ivanyi D. et al, Am. J. Vet. Res. 54: 1095-1102 (1993)
References 4:
Kozaki, M et al, J. Vet. Med. Sci. 63(1): 1-4 (2001)
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Mouse Serum was obtained from healthy animals of Chinese origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Mouse Serum was obtained from healthy animals of Chinese origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkC protein produced in CHO cells was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with human, rat and mouse TrkC. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Growth Associated Protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A dilution of 1:5,000 - 1:20,000 is recommended. A dilution of 1:500-2,000 is recommended for Immunocytochemistry (ICC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on bovine cerebellum homogenate. The molecular weight of the protein recognized can vary (~43-57 kDa) depending on the species and the percentage acrylamide used in the SDS-PAGE gel. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Affinity purified.
Target:
Growth Associated Protein 43 (GAP-43)
Uniprot Number:
P07936
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