Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Ag8.653 myeloma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.
Clone number:
A59
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Applications:
FC
Additional Info:
The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
Clone number:
MEM-18
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
T-47D cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
Clone number:
67A4
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C91/PL (human HTLV-1+ T cell line)
Applications:
FC
Additional Info:
The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Clone number:
C33
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
Product Type:
Staining Reagent
Format:
The reagents in the Amyloid Plaque Stain Reagent (100x) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Application Details:
Staining of amyloid plaques in human and animal tissues, see included protocol
Alternative Names:
AmyloGlo
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
Excitation Peak: 334 nm; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375 nm) Emission (400-450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
Shelf Life:
6 months after date of receipt (unopened vial).
Use:
For research use only.
Kit Components:
5 mL of 100X Amylo-Glo RTD (A-G RTD) solution
Product references:
Silvin A et al. (2022) "Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration" Immunity. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shrader JM et al. (2022) "Distinct Brain Proteomic Signatures in Cerebral Small Vessel Disease Rat Models of Hypertension and Cerebral Amyloid Angiopathy" J Neuropathol Exp Neurol. [Epub ahead of print]; Application: IHC/IF Species: Rat Zagorski K et al. (2022) "Immunogenicity of MultiTEP-Platform-Based Recombinant Protein Vaccine, PV-1950R, Targeting Three B-Cell Antigenic Determinants of Pathological ?-Synuclein" Int J Mol Sci. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shabestari SK et al. (2022) "Absence of microglia promotes diverse pathologies and early lethality in Alzheimers disease mice" Cell Rep. 39(11):110961; Application: IHC/IF Species: Mouse Davis J et al. (2022) "rTg-D: A novel transgenic rat model of cerebral amyloid angiopathy Type-2." Cerebral Circulation - Cognition and Behavior [Epub ahead of print]; Application: IHC/IF Species: Rat Salvadores N et al. (2022) "A? oligomers trigger necroptosis-mediated neurodegeneration via microglia activation in Alzheimer's disease." Acta Neuropathol Commun. 10(1):31; Application: IHC/IF Species: Human Javonillo DI et al. (2022) "Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease." Front Neurosci. 15:785276; Application: IHC/IF Species: Mouse Hohsfield LA et al. (2022) "MAC2 is a long-lasting marker of peripheral cell infiltrates into the mouse CNS after bone marrow transplantation and coronavirus infection." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Tsay HJ et al. (2021) "EK100 and Antrodin C Improve Brain Amyloid Pathology in APP/PS1 Transgenic Mice by Promoting Microglial and Perivascular Clearance Pathways." Int J Mol Sci. 22(19):10413; Application: IHC/IF Species: Mouse Henningfield CM et al. (2021) "Microglia-specific ApoE knock-out does not alter Alzheimer's disease plaque pathogenesis or gene expression." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Da Mesquita S et al. (2021) "Meningeal lymphatics affect microglia responses and anti-A? immunotherapy." Nature. 593(7858):255-260; Application: IHC/IF Species: Mouse Lauterborn JC et al. (2021) "Increased excitatory to inhibitory synaptic ratio in parietal cortex samples from individuals with Alzheimer's disease. Nat Commun. 12(1):2603; Application: IHC/IF Species: Human Kim JH et al. (2021) "Gamma subunit of complement component 8 is a neuroinflammation inhibitor." Brain. 144(2):528-552; Application: IHC/IF Species: Mouse Claes C et al. (2021) "Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease." Mol Neurodegener. 16(1):50; Application: IHC/IF Species: Mouse Crapser JD. (2021) "Investigating microglial regulation of the extracellular matrix in health and neurodegenerative disease." PhD Thesis ; Application: IHC/IF Species: Human Baglietto-Vargas D et al. (2021) "Generation of a humanized A? expressing mouse demonstrating aspects of Alzheimer's disease-like pathology." Nature Communications. 2(1):2421; Application: IHC/IF Species: Mouse Mistrik M et al. (2021) "Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response." Nature Communications. 12, Article Number 719; Application: ICC/IF Species: Human Lemoine L et al. (2020) "Regional binding of tau and amyloid PET tracers in Down syndrome autopsy brain tissue." Mol Neurodegener. 15(1):68; Application: IHC/IF Species: Human Hascup KN et al. (2020) "Riluzole attenuates glutamatergic tone and cognitive decline in A?PP/PS1 mice." J Neurochem. [Epub ahead of print]; Application: IHC/IF Species: Mouse Holloway OG et al. (2020) "Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model. J Alzheimers Dis. 77(4):1765-81; Application: IHC/IF Species: Mouse McQuade A et al. (2020) "Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer s disease. Nat Commun. 11(1):5370; Application: IHC/IF Species: Mouse Hascup KN et al. (2020) "Hippocampal alterations in glutamatergic signaling during amyloid progression in A?PP/PS1 mice." Sci Rep. 10(1):14503; Application: IHC/IF Species: Mouse Crapser JD et al. (2020) "Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain." EBioMedicine. 58:102919; Application: IHC/IF Species: Mouse Abe Y et al. (2020) "Behavioral and electrophysiological evidence for a neuroprotective role of aquaporin-4 in the 5xFAD transgenic mice model." Acta Neuropathol Commun. 8(1):67; Application: IHC/IF Species: Mouse Zhu X et al. (2020) "Robust neuroinflammation and perivascular pathology in rTg-DI rats, a novel model of microvascular cerebral amyloid angiopathy." J Neuroinflammation. 17(1):78; Application: IHC/IF Species: Rat Majewski L et al. (2020) "Transgenic Mice Overexpressing Human STIM2 and ORAI1 in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration." Int J Mol Sci. 21(3); Application: IHC/IF Species: Mouse Davtyan H et al. (2019) "Testing a MultiTEP-based combination vaccine to reduce A? and tau pathology in Tau22/5xFAD bigenic mice." Alzheimers Res Ther. 11(1):107; Application: IHC/IF Species: Mouse Yeh SHH et al. (2019) "A high-sucrose diet aggravates Alzheimer's disease pathology, attenuates hypothalamic leptin signaling, and impairs food-anticipatory activity in APPswe/PS1dE9 mice." Neurbiol. Aging. [In press]; Application: IHC/IF Species: Mouse Bharani KL et al. (2019) "Serum Pro-Bdnf Levels Correlate With Phospho-Tau Staining In Alzheimer's Disease." Neurbiol. Aging. [In press]; Application: IHC/IF Species: Human Hovakimyan A et al. (2019) "A MultiTEP platform-based epitope vaccine targeting the phosphatase activating domain (PAD) of tau: therapeutic efficacy in PS19 mice." Sci Rep. 9(1):15455; Application: IHC/IF Species: Human Hasselmann J et al. (2019) "Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo." Neuron. [Epub ahead of print]; Application: IHC/IF Species: Mouse Spangenberg E et al. (2019) "Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer's disease model." Nat Commun. 10(1):3758 (Supplementary Figure 1); Application: IHC/IF Species: Human Eggers C et al. (2019) "Novel cannabis flavonoid, cannflavin A displays both a hormetic and neuroprotective profile against amyloid _-mediated neurotoxicity in PC12 cells: comparison with geranylated flavonoids, mimulone and diplacone." Biochem Pharmacol. [Epub ahead of print]; Application: IHC/IF Species: Rat Dominguez E (2019) "Microglial Contributions to Alzheimer's Disease Pathogenesis." PhD Thesis, UC Irvine. Application: IHC/IF Species: Mouse Jovic M et al. (2019) "Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model." PLoS One. 14(5):e0216726; Application: IHC/IF Species: Mouse Collins MJ et al. (2019) "Age moderates the effects of traumatic brain injury on beta-amyloid plaque load in APP/PS1 mice." J Neurotrauma. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shukla AK et al. (2018) "CD11a expression distinguishes infiltrating myeloid cells from plaque-associated microglia in Alzheimer's disease." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Feng X et al. (2018) "Quantitative proteomics reveals distinct composition of amyloid plaques in Alzheimer's disease." Alzheimers Dement. [In press]; Application: IHC/IF Species: Human, mouse Davis J et al. (2018) "A Novel Transgenic Rat Model of Robust Cerebral Microvascular Amyloid with Prominent Vasculopathy." Am J Pathol. [Epub ahead of print]; Application: IHC/IF Species: Rat Palombo F et al. (2017) "Detection of A? plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry." Analyst. [Epub ahead of print]; Application: IF Species: Mouse Abud EM (2017) "Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases." PhD Thesis. 2017; Application: IF Species: Mouse Abud EM et al. (2017) "iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases." Neuron. 2017; 49(2):278-93 Application: IF Species: Mouse Solomon IH et al. (2017) "Brain and liver pathology, amyloid deposition, and interferon responses among older HIV-positive patients in the late HAART era." BMC Infect Dis. 2017; 17(1):151 Application: IF Species: Human Xu F et al. (2016) "Cerebral vascular amyloid seeds drive amyloid _-protein fibril assembly with a distinct anti-parallel structure." Nat Commun. 2016; 7:13527. Application: IF Species: Mouse Katsouri L et al. (2016) "PPARγ-coactivator-1_ gene transfer reduces neuronal loss and amyloid-_ generation by reducing _-secretase in an Alzheimer's disease model ." Proc Natl Acad Sci USA. 2016; 113(43):12292-97. Application: IF Species: Mouse Esposito G et al. (2016) "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in _ amyloid-induced neurodegeneration." Sci Rep. 2016; 6: 22605. Application: IF Species: Rat Marsh SE et al. (2016) "The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function." Proc Natl Sci USA. Feb 16. pii: 201525466. Application: IF Species: Hu Fibrillar amyloid visualization. Kim YH et al. (2015) "A 3D human neural cell culture system for modeling Alzheimer's disease." Nat Protoc. Jul;10(7):985-1006. Application: IF Species: Hu , Human neural stem-cell-derived three-dimensional (3D) culture system. Nijholt DA et al. (2015) "Pregnancy Zone Protein is Increased in the Alzheimer's Disease Brain and Associates with Senile Plaques." J Alzheimer's Disease. 46(1):227-38. Application: IF Species: Hu Kamphuis W et al. (2015) "GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease." Glia. Jun;63(6):1036-56. Application: IF Species: Mouse Choi SH et al. (2014) "A three-dimensional human neural cell culture model of Alzheimer's disease." Nature Oct 12. doi: 10.1038/nature1380. Application: IF Species: Hu , Human neural stem-cell-derived three-dimensional (3D) culture system. Niedowicz DM et al. (2014). "Obesity and diabetes cause cognitive dysfunction in the absence of accelerated beta-amyloid deposition in a novel murine model of mixed or vascular dementia." Acta Neuropathol Commun. 2014 Jun 10;2:64.
Specificity:
Amyloid plaques both intraneuronal and vascular
Storage:
The stock solution can be stored for up to 6 months after date of receipt at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
Purification:
Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10,97 | 12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western blot (WB) at a suggested dilution of 1:2,000 - 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Storage:
Store antibody stock solution at 2-8°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
The monoclonal antibody recognizes rat CD8 antigen (MW 34 kDa and 39 kDa) and is reactive with all common inbred strains. 15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single ?-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of proâinflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferonâβ) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
HTA125
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Shimazu; R et al. J Exp Med 1999; 189: 1777
References 2:
Tabeta, K et al Infect Immun 2000, 68: 3731
References 3:
Akashi; S et al. Biochem Biophys Res Commun 2000; 268: 172
The antibody reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm5 was raised against a bacterial fusion protein of MRP1, containing amino acids 986-1204 of the protein. MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRPm5
Concentration:
100 ug/ ml
Format:
Protein G purified
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Cole S et al. Science 1992; 258: 1650-1654
References 2:
Flens M et al. Cancer Res 1994; 54: 4557-4563
References 3:
Zaman et al. Proc Nat Acad Sci 1994; 91: 8822-8826
Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1.28 mg/ml
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Azotobacter vinelandii (Gram-), Bradyrhizobium japonicum, Cyanobacteria, Cyanothece ATCC51142, Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Enterobacter , genera, euryachaeotes, Klebsiella pneumonia, Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp., Mesoorhizobium loti, Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, able to fix atmoshperic nitrogen, Rhizobium melilotiSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.In photobionts like Anabaena sp., low nitrate growth is required to turn on the NifH expression to high enough levels to detect NifH protein. Immunofluorescence protocol Insect dissected tissues (digestive tract, fat body, carrying NifH positive bacteria) of large workers were fixed in cold methanol (20 min, -20 °C) and then permeabilized in cold acetone (5 min, -20 °C). Samples were subsequently rinsed three times with PBS with 0.1 % Triton-X 100 at RT (PBST) and incubated for 5 minutes in PBST. This was followed by incubation of tissues for 1 hr with 6 ug/ml affinity purified anti-NifH antibody (Agrisera, AS01 021A) diluted in PBS-TBSA (PBS, 0.1 % v/v Triton-X-100, 1 mg/ml BSA) and 3 washings with PBST. Samples were then incubated in the dark with a goat anti-chicken IgY conjugated to Dylight 488 (Pierce, SA5-10070) for 45 min and were washed twice (PBS, 0.1%v/v Triton-X-100). Finally, the tissues were mounted in Vectashield medium containing DAPI (Vector Laboratories, H-1500) and viewed under a SP5 Leica confocal microscope with 10X and 63X objectives. Courtesy of Drs. Panagiotis Sapountzis and Mariya Zhukova, University of Copenhagen, Danmark
Application Details:
1 : 500 (IHC), 6 g/ml (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified IgY in PBS pH 8 and 0.02 % sodium azide.
Molecular Weight:
27 | 32.5 kDa
Not reactive in:
Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 as NifH protein is not present in those cyanobacterial species, Frankia sp.
Selected references:
Santana-Sanchez, et al. (2023) Flv3A facilitates O2 photoreduction and affects H2 photoproduction independently of Flv1A in diazotrophic Anabaena filaments. New Phytol. 2023;237(1):126-139. doi:10.1111/nph.18506Chen et al. (2022) Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system, Preprint from Research Square, 22 Jul 2022, DOI: 10.21203/rs.3.rs-1752770/v1Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151He et al. (2021) Vegetative cells may perform nitrogen fixation function under nitrogen deprivation in Anabaena sp. strain PCC 7120 based on genome-wide differential expression analysis. PLoS One. 2021 Mar 4;16(3):e0248155. doi: 10.1371/journal.pone.0248155. PMID: 33662009; PMCID: PMC7932525. (Immunolocalization)Liu et al. (2020). A VIT-like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean. New Phytol . 2020 Mar 2. doi: 10.1111/nph.16506.
The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1.000 and 1:10,000 depending on the method used.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4 C--at least 10 years. Reconstituted at or below -20 C--3-5 years. Reconstituted at +4 C--7 days.
Host Animal:
Goat
Species Reactivity:
Mouse
Immunogen:
Pools of purified homogenous IgG1 isolated from pooled mouse serum, Freund’s complete adjuvant is used in the first step of the immunization procedure,
Applications:
Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins, However this conjugate has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with Human immunoglobulins, This renders it specific for use in test systems containing material of Human origin (e,g, Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate
Conjugate is present in PBS (pH 7,2), No reactivity is observed to other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum
The protein is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of Myostatin expressed in E. Coli.
PARL: presenilin associated, rhomboid-like. This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
4°C -20°C for long term storage
Host Animal:
mouse
Immunogen:
Purified recombinant fragment of PARL (aa112-167) expressed in E. Coli.
The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called âpathogen-associated molecular patternsâ. Activation of TLRs, a family of at least 11 differentmembers that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFNregulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens.<br /> Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
5G5
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Ahmad-Nejad; P et al. Eur J Immunol 2002; 32: 1958
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant His-tagged hamster cyclin B1
Applications:
FC
Clone number:
V152
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Normal human lymphocytes from a lymph node.
Applications:
FC
Additional Info:
The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
Clone number:
3F3
Antibody Isotype:
IgG2b
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
anti-µ-stimulated human B lymphocytes
Applications:
FC
Additional Info:
The mouse monoclonal antibody FN50 recognizes an extracellular epitope of CD69, an lymphocyte early activation marker.
Clone number:
FN50
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody VIPL2 recognizes an extracellular epitope of CD61, a 90-110 kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.
Clone number:
VIPL2
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Cell line KG1a
Applications:
FC
Additional Info:
The mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.
Clone number:
LT56
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human tonsil
Applications:
FC
Additional Info:
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Clone number:
HI186
Antibody Isotype:
IgG2b
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Cultured human NK cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70 kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.
Clone number:
HP-3D9
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Ag8.653 myeloma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.
Clone number:
A59
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Biosite Brand:
BioSite Flow
Conjugation:
PE
Species Reactivity:
human,monkey
EntrezGene ID:
2204
UniProt No:
P24071
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