Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
TFF-MV is a filter cartridge in hollow fibers made of polysulfone. The filter is very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.
Filter cartridge: Polysulfone hollow fibres
Sample volume per reaction: Recommended sample volume from 10 ml up to several liters if connected to mechanical pump
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Storage of lyophilized exosomes: Store up to 1 years at 4°C (Expire date indicated on the label). Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.
Application Details:
NTA in fluorescent mode Flow cytometry. Cell spike- in and in vitro tracking Fluorescence assay calibration EV uptake monitoring, assay and instrument calibration
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
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