MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Storage Temp:
Store at 20 °C or -70 °C upon receipt. After opening maintain undiluted in smaller aliquots at 20 °C or -70 °C for up to 6 months. Avoid multiple freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Storage Temp:
Store antibody stock solution at +4 °C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Western blot (WB) at a suggested dilution of 1:2,000 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Accession Number:
P55145
Reconstitution:
Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally. Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Shelf Life:
12 months after purchase
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. | No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
The Glutathione S-Transferase (GST) family of isozymes function to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione (1). Human GSTs are encoded by 5 gene families, expressing in almost all tissues as four cytosolic and one microsomal forms. Dividing the family by isoelectric points, the basic alpha (pI 811), the neutral mu (pI 57) and acidic pi (pH<5) classes are populated by additional subclasses, each isozyme displaying differential specificity for given electrophilic molecules (2). Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a biomarker for arthritis, asthma, COPD, and multiple forms of cancer, as well as an environmental marker (3-7). Examination of GST isoforms and activity in human cancers, tumors and tumor cell lines has revealed the predominance of the acidic pi class. Furthermore, this activity is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy (8,9).
Product Type:
Assay Kits
Storage Temp:
4ºC
Species Reactivity:
Hu | Mlsk | Fs
Applications:
Fluorometric assay used to quantitatively measure the activity of glutathione S-transferase in samples.
Human | Mollusk | Fish | Toadfish (Batrachoididae) | Oyster
References:
1. Habig, W, et al. J. Biol.Chem. 1974 249(22):7130-7139. 2. Cook, JA, et al. Cancer Res. 1991 51:1606-1612. 3. Dalle-Donne, I, et al. 2006 52(4):601-623. 4. Surapneni, KM & VSC Gopan. Ind.J.Clin.Biochem. 2008 23(1):41.44. 5. Mohan, SK & O Venkataramana. Ind.J.Med. Sci. 2007 61:9-14. 6. Ferrandina, G., et al. Ann. Oncol. 1997 8:343-350. 7. Otitoju, O & Onwarah, INE. African J. Biotech. 2007 6(12)1455-1459. 8. Shea, TC, et al. Cancer Res. 1988 48:527-533. 9. Shea, TC, et al. Cancer Res. 1990 50:6848-6853.
The Glutathione S-Transferase Activity kit is designed to quantitatively measure the activity of GST present in a variety of samples. A GST standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. Mixing the sample or standard with the supplied Detection Reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm. The activity of the GST in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples.
Kit Components:
Black Half Area 96 Well Plate | Glutathione S-Transferase Standard | GST Detection Reagent | Dry DMSO | Assay Buffer | Glutathione (GSH)
Precision:
Intra Assay Precision:Four serum samples were diluted 1:2 with Assay Buffer and run in replicates of 16 in an assay. The mean and precision of the calculated GST activities were: Sample 1- 315.9 mU/mL, 4.6% CV Sample 2- 221.2 mU/mL, 5.6% CV Sample 3- 88.2 mU/mL, 4.2% CV Sample 4- 22.7 mU/mL, 6.6% CV Inter Assay Precision: Four serum samples were diluted 1:2 with Assay Buffer and run in duplicates in twenty assays over multiple days by four operators. The mean and precision of the calculated GST activities were: Sample 1- 291.7 mU/mL, 12.6% CV Sample 2- 218.5 mU/mL, 11.0% CV Sample 3- 89.6 mU/mL, 10.4% CV Sample 4- 23.0 mU/mL, 15.9% CV
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | ICC/IF | IHC
Application Details:
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | ICC/IF | IHC
Application Details:
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms
Immunogen:
A synthetic peptide corresponding to the N-terminal of human DUSP3 (AA 6-19)
Applications:
WB | AM
Application Details:
WB (1:250); optimal dilutions for assays should be determined by the user.
Dual specificity phosphatase 3 antibody, Dual specificity protein phosphatase 3 antibody, Dual specificity protein phosphatase VHR antibody, DUS3_HUMAN antibody, DUSP3 antibody, Serine/threonine specific protein phosphatase antibody, Vaccinia H1-related phosphatase antibody, Vaccinia virus phosphatase VH1 related antibody, VHR antibody
Category:
Polyclonal
Immunogen Species:
Human
Accession Number:
NP_004081
Gene ID:
1845
Swiss-Prot:
P51452
Species Reactivity Full Name:
Human | Mouse
Purification:
Peptide Affinity Purified
Specificity:
Detects 20.478 kDa.
Certificate of Analysis:
A 1:250 dilution of SPC-1122 was sufficient for detection of DUSP3 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms
Immunogen:
A synthetic peptide of human DUSP3 (AA 144-158)
Applications:
WB | AM
Application Details:
WB (1:250); optimal dilutions for assays should be determined by the user.
Dual specificity phosphatase 3 antibody, Dual specificity protein phosphatase 3 antibody, Dual specificity protein phosphatase VHR antibody, DUS3_HUMAN antibody, DUSP3 antibody, Serine/threonine specific protein phosphatase antibody, Vaccinia H1-related phosphatase antibody, Vaccinia virus phosphatase VH1 related antibody, VHR antibody
Category:
Polyclonal
Immunogen Species:
Human
Accession Number:
NP_004081
Gene ID:
1845
Swiss-Prot:
P51452
Species Reactivity Full Name:
Human | Mouse
Purification:
Peptide Affinity Purified
Specificity:
Detects 20.478 kDa.
Certificate of Analysis:
A 1:250 dilution of SPC-1123 was sufficient for detection of DUSP3 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms
Immunogen:
A synthetic peptide corresponding to the C-terminal of human DUSP3 (AA 171-185)
Applications:
WB | AM
Application Details:
WB (1:250); optimal dilutions for assays should be determined by the user.
Dual specificity phosphatase 3 antibody, Dual specificity protein phosphatase 3 antibody, Dual specificity protein phosphatase VHR antibody, DUS3_HUMAN antibody, DUSP3 antibody, Serine/threonine specific protein phosphatase antibody, Vaccinia H1-related phosphatase antibody, Vaccinia virus phosphatase VH1 related antibody, VHR antibody
Category:
Polyclonal
Immunogen Species:
Human
Accession Number:
NP_004081
Gene ID:
1845
Swiss-Prot:
P51452
Species Reactivity Full Name:
Human | Mouse
Purification:
Peptide Affinity Purified
Specificity:
Detects 20.478 kDa.
Certificate of Analysis:
A 1:250 dilution of SPC-1124 was sufficient for detection of DUSP3 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
Rabbit Antiserum
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Ck | Dg | GP | Hm | Pg | Mk | Rb | Sh | Xe
Immunogen:
A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Applications:
WB | IHC | ICC/IF | IP
Application Details:
WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
Rabbit Antiserum
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Ck | Dg | GP | Hm | Pg | Mk | Rb | Sh | Xe
Immunogen:
A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Applications:
WB | IHC | ICC/IF | IP
Application Details:
WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
Synthetic phospho-peptide from human ERK1 + ERK2 proteins around the phosphorylation sites of ERK1 at threonine 202 (pThr202) and tyrosine 204 (pTyr204), and ERK2 at threonine 185 (pThr185) and tyrosine 187 (pTyr187).
Applications:
WB | IHC
Antibody Isotype:
IgG
Application Details:
WB (1:250-1:500); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.
Detects endogenous levels of ERK1 only when phosphorylated at threonine 202 (pThr202) and tyrosine 204 (pTyr204), and ERK2 only when phosphorylated at threonine 185 (pThr185) and tyrosine 187 (pTyr187). Human sites: Thr202+Tyr204/Thr185+Tyr187. Mouse sites: Thr203+Tyr205/Thr183+Tyr185, Rat sites: Thr203+Tyr205/Thr183+Tyr185.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | IHC | ICC/IF | IP | FCM
Application Details:
WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Product Type:
Antibodies
Format:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt | Bv | Mk | Ck | Dg | GP | Hm | Pg | Qu | Rb | Sh | Dr | Xe
Immunogen:
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Applications:
WB | IHC | ICC/IF | IP | FCM
Application Details:
WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Research Area(s):
Cell Signaling | Tags and Cell Markers | Organelle Markers
Target:
Calnexin
Conjugate:
Unconjugated
Alternative Name(s):
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info:
ERK1 or MAPK3 is ubiquitously expressed protein serine/threonine kinase involved in regulating cellular differentiation and proliferation in response to extracellular mitogens or growth factors. MAPK3 is activated by upstream signalling by monomeric G-proteins. MAPK3 activation leads to its translocation from the cytosol to the nucleus where it can target substrate proteins involved in cell cycle regulation; Cyclin D1, suppression of apoptosis; BCL2 and BAD, tumour supression; p53, cell migration; PAI-1, and MHCII mediated immune response; PPARg1. Dysfunction of MAPK3 expression and activity has been observed in a variety of cancers. Predominantly overexpression of MAPK3 or constitutive activation has been observed in cancer cell lines. Activation of MAPK3 does not appear to be due to mutations in the protein itself but rather aberrant signalling in upstream factors including Raf, Ras and epidermal growth factor receptor. MAPK3 may also have a role in promoting metastasis in invasive tumours.
Product Type:
Antibodies
Format:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temp:
-20ºC
Host Animal:
Rabbit
Species Reactivity:
Hu | Ms | Rt
Immunogen:
A phospho-specific peptide corresponding to residues surrounding Tyr204 of human ERK1 (AA 204-209)
Applications:
WB | AM
Application Details:
WB (1:250); optimal dilutions for assays should be determined by the user.
A 1:250 dilution of SPC-974 was sufficient for detection of ERK1 (pTyr204) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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