GPA1 (G PROTEIN ALPHA-1) is involved as a modulator or transducer in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Alternative name: GP-alpha-1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
RACK1A is a major component of RACK1 regulatory proteins playing a major role in multiple signal transduction pathways. Synonymes: Receptor for activated C kinase 1A, WD-40 repeat auxin-dependent protein ARCA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabis alpina, Brasica sp., Camelina sativa, Capsella rubella, Eutrema salsugineum, Ricinus communis, Tarenaya hassleriana Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RACK1A protein sequence O24456, At1g18080. The peptide is also conserved in isofrom RACK1B and RACK1C of Arabidopsis thaliana.
ABP1 is an auxin receptor which regulates polar auxin transport. It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness. ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ABP1 is an auxin receptor which regulates polar auxin transport. It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness. ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ABP1 is an auxin receptor which regulates polar auxin transport. It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness. ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Brown & Jones (1994). Mapping the auxin-binding site of Auxin-binding protein. 1 J Biol Chem 269: 21138-21140, Jones & Herman (1993). KDEL-Containing Auxin-Binding Protein 1 is Secreted to the Plasma Membrane and Cell Wall. Plant Physiol. 101: 595-606, Jones et al. (1991) Red light-regulated growth. I. Changes in the abundance of indoleacetic acid and a 22-kilodalton auxin-binding protein in maize mesocotyl. Plant Physiol 97: 352-358
Malate synthase is involved in the synthesis of (S)-malate from isocitrate, where it catalyses the reaction: acetyl-CoA + glyoxylate + H(2)O = (S)-malate + CoA + H(+). This is part of the glyoxylate cycle, which is itself part of carbohydrate metabolism.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Nicotiana tabacum
Expected Species:
Arabidopsis thaliana, Cajanus cajan, Cinnamomum micranthum f. kanehirae, Cucumis sativus, Cucurbita maxima, Fagus sylvatica, Glycine max, Jatropha curcas, Morus notabilis, Mucuna pruriens, Parasponia andersonii, Populus alba x tremula, Theobroma cacao, Trema orientale Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Cucurbita maxima UniProt: P24571
Experimental contitions: 5 g of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4 C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4 C with agitation. According to South et. al (2019).
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
65 kDa
Not reactive in:
Sorghum bicolor
Selected references:
South et. al (2019). Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science 2019 Jan 4;363(6422), DOI: 10.1126/science.aat9077
Horseradish peroxidase (HRP) is a secretory plant peroxidase that catalyzes the oxidation of small aromatic substrates, such as plant hormone and lignin precursors by hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Brassica campestris, Oryza sativa, Spinacia oleracea, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.
FUM (Fumarase) Fumarate hydratase 1, mitochondrial (FUM1), Fumarate hydratase 2, cytosolic (FUM2) are enzymes which belong to a subpathway of tricarboxylic acid cycle, part of carbohydrate metabolism.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
The antibody may be stored at -20 °C for one year in its original formulation. Additionally, antibody may be stored at 2°Cto 8°Cfor up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Ananas comosus, Capsicum chinense, Cynara cardunculus var. scolymus, Diplotaxis tenuifolia, Eruca versicaria, Genlisea aurea, Glycine soja, Gossypium hirsutum, Helianthus annuus, Hordeum vulgare, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa, Rhizophora mucronata, Ricinus communis, Solanum lycopersicum, Trifolium pratense, Zea mays, Vigna radiata, Quercus suberSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from Arabidopsis thaliana FUM protein sequence, mitochondrial (FUM1): P93033, TAIR: At2g47510, cytosolic (FUM2): UniProt Q9FI53, TAIR: At5g50950
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Diplotaxis tenuifolia,Eruca versicaria, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrium patens, Populus sp., Triticum aestivum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
Hertle et al. (2021). Horizontal genome transfer by cell-to-cell travel of whole organelles. Science Advances. Volume 7. 01 Jan 2021 : eabd8215 DOI: 10.1126/sciadv.abd8215Ancin et al. (2021) Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco. J Exp Bot. 2021 Jun 22;72(13):4949-4964. doi: 10.1093/jxb/erab193. PMID: 33963398; PMCID: PMC8219043.Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Special application note:
This product can be sold contacining proclin if requested
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Silva et al. (2019). Characterization of plant glutamine synthetase S-nitrosation. Nitric Oxide. 2019 Apr 23;88:73-86. doi: 10.1016/j.niox.2019.04.006.Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.
Special application note:
The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. This enzyme is localised in mitochondrial inner membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. botrytis cv. 'Diadom'
Expected Species:
Chlamydomonas reinhardtii, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal mitochondrial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana ATP synthase subunit beta-1 UniProt:P83483, TAIR:AT5G08670ATP synthases subunit beta-2 UniProt:P83484, TAIR: AT5G08690, ATPase subunit beta-3, UniProt: Q9C5A9, TAIR:AT5G08680, which belong to mitochondrial respiratory chain complex I.
Wei et al. (2019). Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis. J Exp Bot. 2019 Aug 6. pii: erz357. doi: 10.1093/jxb/erz357.
Special application note:
Lack of antibody reactivity was confirmed on chloroplast fraction.This product can be sold containing ProClin if requested.
2b protein [Cucumber mosaic virus] is one of the first identified suppressors that could inhibit post-transcriptional gene silencing (PTGS), but with little or no effect on miRNA functions. CMV 2b protein also interferes with miRNA pathways, eliciting developmental anomalies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
2b protein [Cucumber mosaic virus]
Immunogen:
Recombinant 2b protein [Cucumber mosaic virus] Protein accession number: NP_619631.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak
When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38 C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Balfagon et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, note that there might be no HSPs accumulation below temperature of 32-34 C, HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10 C, So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C,Another very effective parameter is the humidity, When using low humidity the plant has a chance to cool down through transpiration, In this case the HSPs induction requires higher temperatures
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25 | 21 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)L mke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Hai-Dong Yu et al. (2012). Downregulation of Chloroplast RPS1 Negatively Modulates Nuclear Heat-Responsive Expression of HsfA2 and Its Target Genes in Arabidopsis. Plos Genetics.
Special application note:
Recommended load per well 20 g of total proteinThis product can be sold containing proClin if requested.
Protein pelota homolog is involved in the mechanism for releasing non-functional ribosomes and degrading damaged mRNA. This protein belongs to the eukaryotic release factor 1 (ERF1) family, and the Pelota subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
PGR5 (Protn gradient regulation 5) is involved in the regulation of the cyclic electron flow (CEF) around Photosystem I and cellular response to light intensity and photoprotection. Essential for the reduction of PGRL1A by ferredoxin and for photoprotection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing proclin if requested
Application Details:
1: 1000 - 1: 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
14,29 kDa
Not reactive in:
diatoms, Chlorella sp., cyanobacteria
Selected references:
Cao et al. (2022) Autophagic pathway contributes to low-nitrogen tolerance by optimizing nitrogen uptake and utilization in tomato. Hortic Res. 2022 Mar 23;9:uhac068. doi: 10.1093/hr/uhac068. PMID: 35669705; PMCID: PMC9164271.Ermakova et al. (2022) Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot. 2022 Jul 29:erac320. doi: 10.1093/jxb/erac320. Epub ahead of print. PMID: 35904136.Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105026Yang et al. (2020). Two dominant boreal conifers use contrasting mechanisms to reactivate photosynthesis in the spring. Nat Commun. 2020 Jan 8;11(1):128. doi: 10.1038/s41467-019-13954-0.Rantala et al. (2020). PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861(3):148154. doi: 10.1016/j.bbabio.2020.148154.
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae (brown and red), Conifers, Cyanobacteria, Brassica napus, Diatoms, Glycine max, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel
Application Details:
1 : 1000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
38 | 28-30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..G recka et al. (2019). Photosystem II 22kDa protein level a prerequisite for excess light-inducible memory, cross-tolerance to UV-C, and regulation of electrical signalling. Plant Cell Environ. 2019 Nov 23. doi: 10.1111/pce.13686.Liu et al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080.
Special application note:
Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.This product can be sold containing ProClin if requested.
PsbC (CP43) acts as an antenna to the PSII core and its presence seem to be also necessary for maintaining water splitting activity. This protein is more weakly associated with the PSII reaction centre and can be removed from the isolated core.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
In C4 plants like Echinochloa crus-galli and Zea mays antibody detects 2 bands.
Application Details:
1 : 3 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
45 | 43 kDa
Not reactive in:
diatoms
Selected references:
Beckova et al. (2022). Photosystem II antenna modules CP43 and CP47 do not form a stable 'no reaction centre complex' in the cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res. 2022 Jan 11. doi: 10.1007/s11120-022-00896-w. Epub ahead of print. PMID: 35015206.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Okegawa et al (2021) Maintaining the Chloroplast Redox Balance Through the PGR5-Dependent Pathway and the Trx System is Required for Light-Dependent Activation of Photosynthetic Reactions. Plant Cell Physiol. 2021 Oct 8:pcab148. doi: 10.1093/pcp/pcab148. Epub ahead of print. PMID: 34623443.Sakuraba at al. (2020). Multilayered regulation of membrane-bound ONAC054 is essential for abscisic acid-induced leaf senescence in rice. Plant Cell. 2020 Jan 6. pii: tpc.00569.2019. doi: 10.1105/tpc.19.00569.Dong et al. (2020). Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana. J Integr Plant Biol. 2020 Jan 15. doi: 10.1111/jipb.12907.
Procaryotic Hsp70 protein family includes DnaK, HscA (Hsc66), HscC (Hsc62). Those proteins are involved in protein folding, cell protection from environmental stress and other functions which are vital for a living cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
It is not determined which isoform of DnaK is recognized by this antibody in Arabidopsis thaliana.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
G hre et al. (2006). One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas The Plant Cell 18:1454–1466.
ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
To induce detectable levels of ERD7, plants need to be exposed to low temperature of 4 C for 24 h.The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
49 | 58 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Barajas-Lopez et al. (2021) EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis. Plant Cell Physiol. 2021 Mar 25;62(1):80-91. doi: 10.1093/pcp/pcaa139. PMID: 33165601.
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
Applications:
Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)
This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, (AS03 037) combined with Rubisco ready to use standard (AS01 017) is recommended.
Application Details:
(IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 g of total cellular protein, (WB)
Purity:
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Guljamow et al. (2021) Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806. Microorganisms. 2021 Jun 10;9(6):1265. doi: 10.3390/microorganisms9061265. PMID: 34200971; PMCID: PMC8230624. (IF)Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1–20. (sea-ice diatom)Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gell rt et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005.Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.
Special application note:
Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots; Nannochloropsis gaditana, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1
For detection on a diatom samples, load of 5-10 g/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dogra et al. (2019). Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6. Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Tietz et al. (2015). Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes. J Biol Chem. 2015 Apr 20. pii: jbc.M114.619841.Campbell et al. (2013). Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana. Photosynth. Res. March 16.
Hydroxypyruvate reductase (HPR) belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family (oxidoreductases) and is involved in glycine, serine and theronine and glyoxylate and dicarboxylate metabolism. Synonymes: HPR, beta-hydroxypyruvate reductase, NADH:hydroxypyruvate reductase, D-glycerate dehydrogenase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Chlamydomonas reinhardtii, Chlorella sp. ,Cucumis sativus, Glycine max, Oryza sativa, Populus albaxtremula, Ricinus communis, VolvoxSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known plant HRP sequences, including Arabidopsis thaliana UniProt: Q9C9W5,TAIR: At1g68010
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Korotaeva et al. (2018). Effect of Heat Hardening on Expression of Genes phb3 and phb4 and Accumulation of Phb Proteins in Green Leaves of Arabidopsis thaliana. Russian Journal of Plant Physiology, 65(5), 688-696, 2018 https://doi.org/10.1134/s1021443718040039Farmer et al. (2013).Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation. Plant Cell, Oct 31.
UniProt number:
Q9C9W5
TAIR number:
AT1G68010
Research area:
Arabidopsis thaliana antibodies
Code:
CM20
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