Rieske Iron-Sulfur Protein (Q9ZR03) is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Kana et al. (2020). Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane. Life (Basel). 2020 Dec 29;11(1):E15. doi: 10.3390/life11010015. PMID: 33383642.Zhang et al. (2020). Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.
Special application note:
This product can be sold containing Proclin if requested.
Sec21p is a constituent of the COPI vesicle coatomer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
98 kDa
Not reactive in:
Nicotiana tabacum, Microsporidia sp.
Selected references:
Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Lupette et al. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research Volume 38, March 2019, 101415.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.Kitakura et al. (2017). BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. doi: 10.1093/pcp/pcx118.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Wang et al. (2016). Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis. J Exp Bot. 2016 Dec;67(22):6323-6335. Epub 2016 Oct 27.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization).Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24.Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
Special application note:
This product can be sold containing proclin if requested
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005). Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17(5):1513-31. Epub 2005 Apr 1.
Application Details:
1 : 50 (IG), 1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Nicotiana tabacum
Selected references:
Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 | doi: 10.3389/fgene.2014.00228
Special application note:
For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Microsporidia sp.
Selected references:
Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Narasimhan et al. (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiol. 2021 Mar 18:kiab134. doi: 10.1093/plphys/kiab134. Epub ahead of print. PMID: 33734402.Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.
Special application note:
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot
PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Chlamydomonas reinhardtii, Chlorella, Oryza sativa, Populus canadensis, Solanum lycopersicum, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from PsaE N-terminal part, conserved in di and monocots and some green algae PsaE protein (not Chlamydomonas), including Arabidopsis thaliana PSI-E A Q9S831, At4g28750 and PSI-E B Q9S714, At2g20260
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Simakawa et al. (2020). Near-infrared in Vivo Measurements of Photosystem I and Its Lumenal Electron Donors With a Recently Developed Spectrophotometer. Photosynth Res. , 144 (1), 63-72 Li et al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Yang et al. (2017). Tetratricopeptide repeat protein Pyg7 is essential for photosystem I assembly by interacting with PsaC in Arabidopsis. Plant J. 2017 Jun 21. doi: 10.1111/tpj.13618.
Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation
Application Details:
1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
56.9 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
Hordeum vulgare Pht1-6 is a putative low-affinity barley phosphate transporter that is likely to function in phosporus remobilisation around the plant (expressed in plant vascular tissues).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Immunogen:
two synthetic peptides from the loop region of Pht1-6 (Q8H6D9), conjugated to KLH
Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Nicotiana tabacum, Zea maysSpecies of your interest not listed? Contact us
Nishioka et al. (2021). Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane. Photosynth Res. 2021 Jan;147(1):107-124. doi: 10.1007/s11120-020-00803-1. Epub 2020 Dec 2. PMID: 33269435; PMCID: PMC7728655.Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Special application note:
This product can be sold containing ProClin if requested
Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).Using this antibody genome editing in Chlorella vulgaris UTEX395 by CRISPR-Cas9 system has been demonstrated as described in Kim et al. (2021)Chemiluminescent detection is advised for NR detection using this antibody.
Costa-Broseta et al. (2021). Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis. Int J Mol Sci. 2021 Jan 7;22(2):E549. doi: 10.3390/ijms22020549. PMID: 33430433.Kim et al. (2021). Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395. Int J Mol Sci. 2021 Jan 6;22(2):E480. doi: 10.3390/ijms22020480. PMID: 33418923.Prinsi et al. (2021). Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability. Plants 10, no. 4: 792. https://doi.org/10.3390/plants10040792Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Zhang et al. (2020). Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10,97 | 12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody can be used to check qubiquitination status in the whole plant extracts.Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20���mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
8,5 kDa
Not reactive in:
Algae
Selected references:
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Filippi et al. (2019). Caspase-3-like activity and proteasome degradation in grapevine suspension cell cultures undergoing silver-induced programmed cell death. J Plant Physiol. 2019 Feb;233:42-51. doi: 10.1016/j.jplph.2018.12.003.Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]
Special application note:
Ubiquitin mutant is lethal, therefore could not be used in validation of this antibody.
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Sorghum bicolor, Zea mays
Expected Species:
Hordeum vulgare, Oryza sativa Species of your interest not listed? Contact us
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Brassica campestris, Oryza sativa, Spinacia oleracea, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.
CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Diplotaxis tenuifolia,Eruca versicaria, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrium patens, Populus sp., Triticum aestivum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
Hertle et al. (2021). Horizontal genome transfer by cell-to-cell travel of whole organelles. Science Advances. Volume 7. 01 Jan 2021 : eabd8215 DOI: 10.1126/sciadv.abd8215Ancin et al. (2021) Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco. J Exp Bot. 2021 Jun 22;72(13):4949-4964. doi: 10.1093/jxb/erab193. PMID: 33963398; PMCID: PMC8219043.Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Special application note:
This product can be sold contacining proclin if requested
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Silva et al. (2019). Characterization of plant glutamine synthetase S-nitrosation. Nitric Oxide. 2019 Apr 23;88:73-86. doi: 10.1016/j.niox.2019.04.006.Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.
Special application note:
The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
38 | 38 kDa
Not reactive in:
Synechocystis sp.
Selected references:
Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20 °C; once reconstituted to a final volume this antibody can be kept in 4°Cfor up to one year, in smaller portions to avoid contamination. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody will not recognize any other cereal hsp101
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak
When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38 C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Balfagon et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, note that there might be no HSPs accumulation below temperature of 32-34 C, HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10 C, So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C,Another very effective parameter is the humidity, When using low humidity the plant has a chance to cool down through transpiration, In this case the HSPs induction requires higher temperatures
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25 | 21 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)L mke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Hai-Dong Yu et al. (2012). Downregulation of Chloroplast RPS1 Negatively Modulates Nuclear Heat-Responsive Expression of HsfA2 and Its Target Genes in Arabidopsis. Plos Genetics.
Special application note:
Recommended load per well 20 g of total proteinThis product can be sold containing proClin if requested.
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) UniProt: P13853, TAIR: At1g53540
There are six total class I genes, Essentially this antibody might react to some extent with all of them, But does not react with class II, organelle, or any other shsp classes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Nicotiana tabacum, Vitis vinifera Species of your interest not listed? Contact us
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Expected Species:
Chlamydomonas reinhardtii, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
full length recombinant ycf4 protein of Chlamydomonas reinhardtii UniProt: O20030 as described in Boudreau et al. 1997
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
UniProt number:
O20030
Research area:
PSI (Photosystem I)
Code:
Photo20
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