The Ki67 antigen is a nuclear protein which is expressed in all active parts of the cell cycle (G1, S, G2 and mitosis) but is absent in resting cells (G0). In contrast to many other cell cycle-associated proteins, the Ki67 antigen is consistently absent in quiescent cells and is not detectable during DNA repair processes. Thus, the presence of Ki67 antigen is strictly associated with the cell cycle and confined to the nucleus, suggesting an important role in the maintenance and/or regulation of the cell division cycle.
Antibody Isotype:
IgG1
Monosan Range:
MONXtra
Clone:
K2
Concentration:
n/a
Storage buffer:
Tissue culture supernatant with 15 mM sodium azide
c-Myc is a phosphoprotein involved with cell proliferation and differentiation. It is a useful marker for differentiation between Burkitt's lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) since, despite morphological similarities between the two B-cell lymphomas, Anti-c-Myc stains all BL and only a few DLBCL cases. A panel of antibodies against c-Myc, CD10, BCL2, and Ki-67 is useful for cases where Myc FISH analysis is warranted or can be omitted. Nuclear c-Myc overexpression is common in luminal cells of prostate intraepithelial neoplasia, many primary carcinomas, and metastatic disease.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Ki67, also known as MKI67, is aprototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation
Ki67, also known as MKI67, is aprototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation
The Ki67 antigen is a nuclear protein which is expressed in all active parts of the cell cycle (G1, S, G2 and mitosis) but is absent in resting cells (G0). In contrast to many other cell cycle-associated proteins, the Ki67 antigen is consistently absent in quiescent cells and is not detectable during DNA repair processes. Thus, the presence of Ki67 antigen is strictly associated with the cell cycle and confined to the nucleus, suggesting an important role in the maintenance and/or regulation of the cell division cycle.
Chicken anti-Ki-67 Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Ki-67 protein (mixture of amino acids 1-300 and 1,111-1,490) expressed in and purified from <i>E. coli.</i>
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:2,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human only. Does not react with mouse or rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2 and M), but not in the resting phases (G0 and G1 early phase). Although the antigen has also been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has thus been indicated as an effective marker in grading the proliferation rate of tumours, including those of the brain, breast, cervix, and prostate.
c-Myc is a phosphoprotein involved with cell proliferation and differentiation. It is a useful marker for differentiation between Burkitt's lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) since, despite morphological similarities between the two B-cell lymphomas, Anti-c-Myc stains all BL and only a few DLBCL cases. A panel of antibodies against c-Myc, CD10, BCL2, and Ki-67 is useful for cases where Myc FISH analysis is warranted or can be omitted. Nuclear c-Myc overexpression is common in luminal cells of prostate intraepithelial neoplasia, many primary carcinomas, and metastatic disease.
Human Ki67 ELISA Kit (96 Tests). Quantitate Human MKI67 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 1pg/ml.
Product Type:
Assay & Detection
Storage Temp:
Mixed
Immunogen:
Expression system for standard: E.coli; Immunogen sequence: N3120-I3256
Applications:
ELISA
Additional Info:
For quantitative detection of human Ki67 in cell culture supernatants, serum and plasma (heparin, EDTA).
Biosite Brand:
BioSite ELISA
Species Reactivity:
human
Cross Reactivity:
There is no detectable cross-reactivity with other relevant proteins.
EntrezGene ID:
4288
UniProt No:
P46013
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