SAE1a (SUMO-activating enzyme subunit 1A) is the dimeric enzyme acts as an E1 ligase for SUMO1 and SUMO2. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Functionally redundant with its paralog SAE1B. Alternative names: SUMO-activating enzyme subunit 1-1, Ubiquitin-like 1-activating enzyme E1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Cajanus cajan, Cicer arietinum, Noccaea caerulescens, Parasponia andersonii, Trema orientale Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length SAE1a of Arabidopsis thaliana, UniProt:Q8VY78-1, TAIR: At4g24940, overexpressed in E.coli
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Saracco et al. (2007). Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145(1):119-34.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse
Immunogen:
A peptide from the C-terminus of mouse C-reactive protein (210-225 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
C-reactive protein Mouse
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
E3 reacts with keratin 17 (45 kDa). The antibody reveals myoepithelial cells, basal cells and proliferating epithelia in some benign epithelial tumors as well as malignant carcinomas.
EBS-huKappa reacts with kappa light chain In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
EBS-huKappa
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Takahashi, H. et al. Pathol Res Prac 189, 300-311 (1993)
References 2:
Momose, H., et al. Hum. Pathol 23: 1115-1119 (1992)
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. <br><br>Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
L.Y. Chen et al (2010) Learning induces neurotrophin signaling at hippocampal synapses Proc Natl Acad Sci USA. Apr 13;107(15):7030-5 Soderquist R.G. et al (2009) PEGylation of brain-derived neurotrophic factor for preserved biological activity and enhanced spinal cord distribution J Biomed Mater Res A. 2009 Dec;91(3):719-29. Tang S. et al (2010) Immunolocalization of pro- and mature-brain derived neurotrophic factor (BDNF) and receptor TrkB in the human brainstem and hippocampus. Brain Res. Oct 1;1354:1-14. Sadri-Vakili G. et al (2010) Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. J Neurosci. 2010 Sep 1;30(35):11735-44. Maldonado M.A. et al (2008) Motor skill training, but not voluntary exercise, improves skilled reaching after unilateral ischemic lesions of the sensorimotor cortex in rats. Neurorehabil Neural Repair. 2008 May-Jun;22(3):250-61. Nakajima H. et al (2007) Rescue of rat anterior horn neurons after spinal cord injury by retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene. J Neurotrauma. 2007 Apr;24(4):703-12. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains. J Histochem Cytochem. 2007 Jan;55(1):1-19. Carrasco M.A. et al (2007) Regulation of glycinergic and GABAergic synaptogenesis by brain-derived neurotrophic factor in developing spinal neurons. Neuroscience. 2007 Mar 16;145(2):484-94. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Mouse anti-Rhodopsin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Purified bovine rhodopsin
Applications:
IHC-Frozen,WB
Clone number:
B630
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000) and Immunohistochemistry (1:1,000). Due to the highly hydrophobic nature of rhodopsin, avoid boiling samples in SDS-PAGE sample buffer for rhodopsin analysis by Western Blotting, as this will result in extensive aggregation of the rhodopsin protein and appearance of high molecular weight bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Bovine,reacts with Human, Rat, Mouse, Cow, Pig. Expected to react with other mammalian species.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
recombinant rat PCNA
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.
Androgen Receptor (AR) is a transcriptional regulator with a broad array of functions. This marker is clinically significant in the understanding of tumour progression and tumour aggressiveness. The detection of AR by immunohistochemical staining is important for diagnosis of all types of prostate carcinoma, including both therapy-responsive and therapy-unresponsive disease states. Co-testing with AR and CK20 is used for differential diagnosis of desmoplastic trichoepithelioma (DTE) [CK20+/AR-], morpheaform basal cell carcinoma (BCC) [CK20-/AR+], and microcystic adnexal carcinoma (MAC) [CK20-/AR-].
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC511
Antibody Isotype:
IgG
GMDN Code:
56796
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Prostate Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
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