C-46 reacts with keratins 7 (54kDa) and 17 (46kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-46
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Bártek et al. J. of Pathol. 164: 215-24 (1991)
References 2:
Vojt?ek et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Taylor Papadimitriou J. et. al. J. Cell Sci. 94: 403-13 (1989)
References 4:
Kova?ík et al. Int. J. Cancer Suppl. 3: 50-55, (1988)
References 5:
Kova?ík et al. J. Tumor Marker Oncol. 5: 219, (1990)
C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Antibody Isotype:
IgG1-K
Monosan Range:
MONOSAN
Clone:
C-11
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Vojt?ek, B. et al. Folia Biol., 35(6): 373-382, (1989)
References 2:
Vojt?ek, B. et al. Neoplasma 37(3): 333-342 (1990)
References 3:
Kova?ík J. et al. Int. J. Cancer, Suppl. 3: 50-55, (1988)
References 4:
Kova?ík J. et al. J. Tumor Marker Oncol. 5: 219 (1990)
CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
COS-7 (African green monkey) cells
Applications:
FC,WB,IHC
Additional Info:
The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Clone number:
MEM-122
Antibody Isotype:
IgM
Application Details:
Flow cytometry: Recommended dilution: 2 ?g/ml. Western blotting: Non-reducing conditions.
CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human peripheral blood lymphocytes
Applications:
FC
Additional Info:
The antibody LT27 reacts with an extracellular epitope of CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
Clone number:
LT27
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: Recommended dilution: 2 ?g/ml.
Biosite Brand:
BioSite Flow
Conjugation:
Unconjugated
Concentration:
1 mg/ml
Species Reactivity:
human
EntrezGene ID:
939
UniProt No:
P26842
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