The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
RAb-50 reacts with SAD-Vnukovo and Pitman-Moore strains of rabies virus. It shows conformation dependent specificity for the viral envelope glycoprotein. In immunofluorescence test, SAD-Vnukovo strain is recognized by the antibody, while in Western blot, both strains are recognized by the antibody. In ELISA only SAD-Vnukovo strain is recognized. RAb-50 can neutralize the CVS strain of rabies virus.
Antibody Isotype:
IgG2b
Monosan Range:
MONOSAN
Clone:
RAB-50
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Macikova, I. et al, Acta virol. 36: 541-550 (1992)
References 2:
Sajjanar B, et al, Neuropeptides. 57: 59-64 (2016)
References 3:
Chen Li, et al, Acta Pharmacol Sin. 32(3): 329337 (2011)
Polyhisitidine tagged proteins (N- or C-terminus label)
Concentration:
DyLight® 550
Form:
Liquid
Purification:
Affinity Purified Using solid phase 6x Histidine IgG
Buffer:
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Human influenza hemagglutinin (HA) tagged proteins
Concentration:
1mg/ml
Conjugate:
DyLight® 550 (Ex = 562 nm; Em = 576 nm)
Form:
Liquid
Purification:
Affinity Purified Using solid phase HA
Buffer:
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Human influenza hemagglutinin (HA) tagged proteins
Concentration:
1mg/ml
Conjugate:
DyLight® 650 (Ex = 652 nm; Em = 672 nm)
Form:
Liquid
Purification:
Affinity Purified Using solid phase HA
Buffer:
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al. , 2012 , followed by lyophilisation using Biosensis' proprietary stabilization procedures. The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric A? 1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assays, as well as other research applications.
Product Type:
Peptide
Format:
Lyophilized, Supplied as 2 x 500 ng vials, each containing lyophilized A? oligomers</b>. Note that the amount of provided oligomeric protein is based on the amount of monomeric A? used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
Applications:
ELISA
Application Details:
<i>Use as positive control in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.<br><br><i>Use as oligomeric A-beta peptide standard in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1750-1000_Instructions for Generating a Calibration Curve.pdf"> here </a> for detailed instructions on generating a calibration curve with PE-1750-1000.<br><br><i>Use as positive control in other applications</i>: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 - 200 µL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.
Store unopened, lyophilized oligomeric A? with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2-8°C for up to 2 days. The reconstituted material should not be frozen for best results.
Laminin is a glycoprotein (Mr 850 - 1.000 kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses. <br />No cross reaction was obtained with human type I, III, IV and V collagen in immunoblotting, whereas the antibody reacted with a distinct band of appr. 200-220 kD from a 8M Urea extract from amnion basement membrane. Positive control: skin, kidney.
GIP occurs in endocrine cells in the small intestine. GIP is released upon feeding, particularly after carbohydrate-rich food, and is known to sensitize the insulin cells to rise in blood sugar and is thus involved in the insular axis. GIP also inhibits gastric acid secretion.<br> Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while CCK-39, VIP and secretin do not.
Monosan Range:
MONOSAN
Concentration:
n/a
Storage buffer:
Lyophilized; reconstitute in 50-100 ul dist. water (final solution contains 0.09% sodium azide, 1% BSA in PBS buffer, pH 7.4)
Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane. The antiserum is raised against the C-terminus of rat NIS. Suitable for studying the sensitivity of thyroid tumors to iodine treatment. Absorption with 50-100 ug immunogen per ml diluted antiserum abolishes staining. Positive control: frozen sections of rat thyroid.
Rabbit anti-Sortilin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with LPL and SLC2A4. Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin. TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. INDUCTION: During osteoblast differentiation. DOMAIN: The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding. DOMAIN: The extracellular domain may be shed following protease cleavage in some cell types. PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. PTM: Contains 8 intrachain disulfide bonds. PTM: N-glycosylated. SIMILARITY: Contains 9 BNR repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human sortilin produced in CHO cells was used as the immunogen.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA (1:5000 dilution). A dilution of 1:1000 is recommended for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. Reacts with human, rat and mouse Sortilin. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Endothelin B receptor (ET-BR) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Endothelin B receptor is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. It acts as a non-specific receptor for endothelin 1, 2, and 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from human Endothelin B receptor (299-313 aa).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB. Suggested dilution of 1:1,000-1:5,000. Human Endothelin B receptor has a predicted length of 442 residues and MW of 50 kDa. Biosensis recommends that the optimal working dilution should be determined by the end user.
Human influenza hemagglutinin (HA) tagged proteins
Concentration:
DyLight® 550
Form:
Liquid
Purification:
Affinity Purified Using solid phase HA
Buffer:
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Human influenza hemagglutinin (HA) tagged proteins
Concentration:
1mg/ml
Conjugate:
DyLight® 650 (Ex = 652 nm; Em = 672 nm)
Form:
Liquid
Purification:
Affinity Purified Using solid phase HA
Buffer:
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
100 mM NaPO4 (pH 7.4), 100 mM NaCl, 2 mM Sodium Azide
Storage:
-20C or colder
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
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