Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1,863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Control tissue Pancreas, breast carcinoma, ovarian carcinoma. Staining Nuclear and cytoplasmic.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
C-4
Concentration:
n/a
Format:
Concentrate
Storage buffer:
PBS with < 0.1% sodium azide and 0.1% gelatin
Storage:
2-8°C
References 1:
Hall, J.M., et al. 1990, Science 250: 1684-1689.
References 2:
Yoshikawa,Y., et al. 2012, Cancer Sci. 103: 868-874.
References 3:
Gammon, B., et al. 2013, J. Cutan. Pathol. 40: 538-542.
References 4:
Kerl, K., et al. 2013, Am. J. Dermatopathol. 35: 151-158.
References 5:
Popova T., et al. 2013, Am. J. Hum. Genet. 92: 974-980.
Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Arabidopsis thaliana, Manihot esculenta, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.
Applications:
Co-Immunoprecipitation (IP) (Co-IP), Immunolocalization (IL), Western blot (WB)
Important note: For blocking 5 % skim milk in PBS without Ca++ should be used,This antibody is purified by affinity chromarography on Portein G
Application Details:
8 g/ml (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
35, 34 | 32 and 34 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mansilla et al. (2020).- Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants. Eur J Plant Pathol 158, 211?222 (2020). https://doi.org/10.1007/s10658-020-02067-wColman et al. (2019). Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry Volume 143, October 2019, Pages 203-211. Kumari et al. (2017), Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Front Plant Sci. 2017 Jan 6;7:2027. doi: 10.3389/fpls.2016.02027.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ?Penncross?)Ko et al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.
Special application note:
Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.
The antibody reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm5 was raised against a bacterial fusion protein of MRP1, containing amino acids 986-1204 of the protein. MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRPm5
Concentration:
100 ug/ ml
Format:
Protein G purified
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Cole S et al. Science 1992; 258: 1650-1654
References 2:
Flens M et al. Cancer Res 1994; 54: 4557-4563
References 3:
Zaman et al. Proc Nat Acad Sci 1994; 91: 8822-8826
MRPr1 reacts with an epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPr1 was raised against a bacterial fusion protein of MRP, containing a segment of 168 amino acids in the amino-proximal half of the protein. MRPr1 does not cross-react with the human MDR1 and MDR3 gene products (Flens et al. 1994).
Antibody Isotype:
IgG2a
Monosan Range:
MONOSAN
Clone:
MRPr1
Concentration:
250 ug/ ml
Storage buffer:
Serum free tissue culture supernatant with 0.7% BSA and 0.1% sodium azide
Storage:
2-8°C
References 1:
Cole S et al. Science 1992; 258: 1650-1654
References 2:
Flens M et al. Cancer Res 1994; 54: 4557-4563
References 3:
Zaman et al. Proc Nat Acad Sci 1994; 91: 8822-8826
The Bombesin Antibody was raised to synthetic human bombesin coupled to bovine thyroglobulin with glutaraldehyde. The ImmunoStar bombesin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400-1/600 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Technical details how to work with this antibody are provided here: P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Special application note:
This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
HTF-14
Concentration:
1 mg/ml
Format:
Purified by protein-A affinity chromatography.
Storage buffer:
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage:
2-8°C
References 1:
Bartek J., et al., Immunol. Lett. 1982; 7: 231.
References 2:
Bartek, J., et al., Br. J. Haematol 1985; 59: 435-441.
References 3:
Nováková, M., et al., Cell Motil Cytoskeleton 1996;33(1):38-51
EBS-IF-004 reacts specifically with keratin 14 (50kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.
Antibody Isotype:
IgM-K
Monosan Range:
MONOSAN
Clone:
EBS-IF-004
Concentration:
100 ug/ml
Storage buffer:
PBS with 0.02% sodium azide
Storage:
2-8°C
References 1:
Ivanyi, D. et al. Am. J. Vet. Res. 53: 304-314 (1992)
References 2:
Ivanyi, D. et al. Cancer Res. 50(16): 5143-5152 (1990)
References 3:
Balm A.J.M. et al., Eur. Arch. Otorhinolaryngol. 253: 227-233 (1996)
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