Chicken anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid: Concentrated ammonium sulphate in PBS pH 7.4
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:250 to 1:500 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cadherin family member 12; Ret;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human RET
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Purification:
Affinity purified
Target:
Proto-oncogene tyrosine-protein kinase receptor Ret (RET)
Chicken anti-GDNF family receptor alpha-4 (GFR alpha-4) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
GFR_-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7.4 containing no preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFRa-4 protein produced using CHO-based cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
WB, IHC. A dilution of 1:5 000-1:10 000 is recommended for Western blots and 1:1 000 to 1:3 000 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Chicken anti-GDNF family receptor alpha-3 (GFR alpha-3) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 contain no preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:1500 to1:3000 for immunocytochemistry. Biosensis recommends that optimal dilutions/concentrations should be determined by the end user.
Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-GDNF family receptor alpha-2 (GFR alpha-2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFR_-2 is a member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NRTN compared to its other family member, GDNF family receptor alpha 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFR alpha-2 protein produced using CHO-based cell line. For production of hGFR alpha-2, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:500 to 1:1,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
human GFR alpha-2. No cross reaction with hGFR alpha-1, hGFR alpha-3, hGFR alpha-4
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-GDNF family receptor alpha-1 (GFR alpha-1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFR_-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:1,000 to 1:2,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-mCherry Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Foerster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Species Independent
Immunogen:
Recombinant full length mCherry.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,000 - 1:5,000 isC recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a band at ~28-30 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Growth associated protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Other Mammals (Predicted),Rat
Immunogen:
C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
neuron growth-associated protein 43; neuromodulin; nerve growth-related peptide GAP43; axonal membrane protein GAP-43; protein F1; calmodulin-binding protein P-57; neural phosphoprotein B-50; Growth Associated Protein 43; GAP43;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It is expected that it will work on other mammal tissues due to amino acid sequence similarity.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two mouse beta NGF peptides (74-85 and 113-124 aa).
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:1,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human beta NGF, cross reactivity to other species NGF is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Dicer is the rate limiting enzyme in the formation of mature microRNAs.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, 0.02% sodium azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:3,000. Mouse Dicer Protein has a predicted length of 1916 residues and the MW of the monomer is 218 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Specificity:
Specificity confirmed by IHC and WB in mouse brain. Mouse; Rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Chicken anti-Mitochondrial uncoupling protein 3 (UCP 3) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Uncoupling Protein 3 (UCP3) belongs to the mitochondrial carrier family. Located in the mitochondrion inner membrane, UCP3 creates proton leaks across the membrane thus uncoupling oxidative phosphorylation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from the C-terminus of human Uncoupling Protein 3 (298-312 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
UCP3; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; SLC25A9;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects UCP3 at approx 35 kDa. Human, Mouse, Rat, Porcine
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Transforming growth factor beta-1 (TGFB1) is a multi-functional cytokine with roles in proliferation, differentiation and other functions in many cell types. The secreted TGFB1 protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from human and mouse Transforming growth factor beta-1 (372-386 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
TGFB1; TGFB; TGF-beta-1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
TGF beta 1, cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Rat
Immunogen:
A peptide from rat C-reactive protein (108-121 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat C-reactive protein
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody) .
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-2 (PS-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-2 (PSEN2) is a multi-pass membrane protein and component of the gamma-secretase complex. Defects in PSEN2 are a cause of Alzheimer disease type 4 (AD4), an autosomal dominant Alzheimer disease. (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 2 peptides (319-330 and 349-360 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-1 (PS-1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 1 peptides (311-322 and 341-352 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Presenilin 1
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear hormone receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Peroxisome proliferator-activated receptor gamma peptide (115-129 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:2,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
PPAR gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Peroxisome proliferator-activated receptor gamma
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Orexin B Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin B (1-14 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Orexin A Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin A (1-13 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Obestatin Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Obestatin is generated from the proteolytic cleavage of Ghrelin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
Obestatin peptide (76-98 aa) from mouse ghrelin precursor.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:500 to 1:2,000 in coating buffer as coating antibody in competitive ELISA testing. Biosensis recommends that the optimal working dilution should be determined by the end user.
Beta-adrenergic receptors are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family. Their function is to mediate the catecholamine-induced activation of adenylate cyclase through the action of G-proteins (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse beta 3 Adrenergic Receptor (386-400 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Fatty acid-binding protein, adipocyte peptide peptides (71-82 aa, 97-111 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Fatty acid-binding protein, adipocyte peptide (97-111 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two peptides from human CNTF (11-25 and 186-200 aa). Both peptides are highly conserved among human and mouse.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA at a suggested dilution of 1:500 to 1:2,000. A titration between 1:50 to 1:500 is suggested for IHC. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor; CNTF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CNTF, Cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Appetite-regulating hormone, active (Active Ghrelin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human active Ghrelin peptide (24-33 aa) S3n-octanoicacid covalently linked to amino acid 28
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:500 to 1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Adiponectin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Adiponectin is synthesized by adipocytes and is involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from human Adiponectin (230-244 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
HSL; LIPE; Hormone-sensitive lipase; Lipe;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects HSL at approx 83 kDa in various fat cell lysates from mouse and rat. Additional weaker band may appear at approx 40 kDa (unknown). Mouse, Rat, Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
Chicken anti-Rhodopsin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Rod shaped photoreceptor cells that are required for image-forming vision at low light intensity and for photoreceptor cell viability after birth (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Purified chicken IgY. Liquid in PBS with 0.02% Sodium Azide. Concentration 1 mg/mL.
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat,Tiger Finch,Zebra Finch
Immunogen:
Mixture of synthetic peptide (14-26 aa) of Mouse Green cones Opsin 1 and synthetic peptide (18-30 aa) of Tiger finch red cones opsin 1 was used as immunogen.
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:500 to 1:2,000. The antibody recognizes a band of approximately 37 kD on mouse brain lysate. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Rho; Green cone; Red cone; opsin1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse, Rat, Tiger Finch, Zebra finch
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-G-Protein B3 (Rho) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
G-Protein b3 (GNB3) is a guanine nucleotide-binding protein (G protein). G-proteins are involved as a modulator or transducer in various transmembrane signaling systems (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human GNB3 peptide (216-230 aa)
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Appetite-regulating hormone (Grehlin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide from human Ghrelin, C-terminal, (17-28 aa)
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human GDNF peptides (101-118 and 199-211 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:500. Biosensis recommends that the optimal working dilution should be determined by the end user.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human BDNF peptides (73-87 and 194-208 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Acetyl-lysine Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Lysine acetylation of histones and non-histone proteins plays an important part in many cellular processes such as chromatin and nuclear signaling, transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Species Independent
Immunogen:
Acetylated Lysine OVA
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Acetyl-lysine;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Binds to proteins with acetylated lysine residues.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene) .
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT4/NT5 peptides (104-118 and 198-210 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Neurotrophin-3 (NT-3) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT3 peptides (90-104 and 199-214 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse
Immunogen:
A peptide from the C-terminus of mouse C-reactive protein (210-225 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
C-reactive protein Mouse
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Purified human C-reactive protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human C-reactive protein Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Vimentin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human Vimentin purified from E.coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000-5,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
VIM;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~50 kDa Vimentin enzyme. Hu, Rat, Ms. It is predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals,Rat
Immunogen:
Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Fel, and other mammals
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (ref: SWISSPROT). Pin1 is concentrated in the nucleus in small punctate structures and is particularly obvious in tumor cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Neurofilament medium polypeptide (NF-M) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000. Shaw G. et al (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites. Biochem Biophys Res Commun. 2004 Dec 10;325(2):619-25.
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Purified porcine NF-L from spinal cord and recombinant NF-L.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody binds primarily to the phosphorylated axonal forms of NF-H.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilution for WB is 1:20,000-50,000. Suggested dilution for ICC/IHC is 1:20,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000. Shaw G. et al (2005) Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336(4):1268-77.
Specificity:
This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
IgY
Target:
Neurofilament heavy polypeptide, phosphorylated (pNF-H)
Chicken anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Three peptide sequences conserved in higher verterbrate MBP protein.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Hu, Rat, Ms, Bov. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Microtubule-associated protein 2 (MAP2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Bovine MAP2 isolated from brain by the GTP microtubule cycling method.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilutions are: 1:10,00-1:20,000 (WB), 1:5,000-1:10,000 (ICC, IHC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Microtubule-associated protein 2; MAP-2; MAP2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Michurina A (2021) "Loss of Setd1b methyltransferase in the murine forebrain as a novel model for human intellectual disability." PhD Thesis. Application: IHC (IF) Species: Mouse. Iwata M et al. (2019) "Regulatory mechanisms for the axonal localization of tau protein in neurons." Mol Biol Cell. 30(19):2441-57. Application: ICC/IF Species: Mouse, rat. Duda JK et al. (2019) "The role of DLG-MAGUKs in mediating signaling specificity at the postsynaptic density." PhD Thesis. [Epub ahead of print]. Application: ICC/IF Species: Mouse. Awashti A et al. (2018) "Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting." Science. 2018; [Epub ahead of print]. Application: IHC/ICC/IF Species: Mouse. Wolfes AC et al. (2016) "A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes." J Gen Physiol. 2016; [Epub ahead of print]. Application: IF Species: Rat. Dziennis S et al. (2007) "Role of signal transducer and activator of transcription-3 in estradiol-mediated neuroprotection." J Neurosci. 2007; 27(27):7268-74. Application: IHC Species: Rat.
Specificity:
The specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Bov
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant rat alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Background Info:
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Hascup KN et al. (2020) Riluzole attenuates glutamatergic tone and cognitive decline in A?PP/PS1 mice. J Neurochem. [Epub ahead of print] Application: Mouse, IHC(IF).
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Shortest isoform of recombinant full length CK1a.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB): 1:5,000 - 1:10,000. Casein kinase 1 alpha has a predicted molecular weight of 38 kDa. <br><br>Immunocytochemistry (ICC): 1:500-1:1,000.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CK1; CKI-alpha;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
BL-005-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-005-500: BEK-2212, Human NGF; Application: Citrate-Plasma One vial of BL-005-500 contains 500 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use mouse assay antibodies may also benefit from addition of blocker BL-005-500, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2212 and similar ELISA assays
BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-004-500: BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA) BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma One vial of BL-004-500 contains 500 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-003-1000: BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma BEK-2218, Human NT4/5; Application: Citrate-Plasma One vial of BL-003-1000 contains 1000 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2226 & BEK-2218 and similar ELISA assays
BL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. One vial of BL-002-1250 contains 1250 ?g IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 ?g/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays utilizing mouse antibodies.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2219 and similar ELISA assays
BL-001-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce the sIgA cross-reactivity present in certain substrates such as milk. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-001-1250: BEK-2211, Mature BDNF; Application: human milk One vial of BL-001-1250 contains 1250 ?g of proprietary immunoglobulins, which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays may also benefit from addition of blocker BL-001-1250, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate secretory IgA (sIgA) interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Secretory IgA immunoassay Blocker for BEK-2211 and similar ELISA assays
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein
The Biosensis Mature BDNF/proBDNF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF ( BEK-2211 ) and full-length proBDNF ( BEK-2237 ). Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate and EDTA), mouse and rat cell lysates and rat brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2211 (Mature BDNF Rapid TM ELISA) and BEK-2237 (proBDNF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. BEK-2241 is the preferred Combo kit for human blood samples. The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance. Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
See BEK-2211 & BEK-2237 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature BDNF and proBDNF in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x BDNF antibody, 1 x proBDNF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2211 (Mature BDNF Rapid TM ELISA Kit) and BEK-2237 (proBDNF Rapid TM ELISA Kit) .
Specificity:
Mature BDNF ELISA: Human, mouse, rat BDNF and numerous other species. Although the BDNF assay antibodies are raised against the mature domain, the mature BDNF ELISA preferentially detects mature BDNF over full-length proBDNF.
proBDNF ELISA: Human, mouse and rat proBDNF. The capture antibody used in the proBDNF ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF, and does not quantify mature BDNF.
Typical limit of detection (LOD) for mature BDNF is less than 2 pg/mL, and 6 pg/mL for proBDNF determined as 150% of the blank value.
Cross Reactivity:
<b>Cross-reactivity of BDNF isoforms:</b><br><br><b>Mature BDNF ELISA:</b><br>No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM).<br><br><b>proBDNF ELISA:</b><br>Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration. The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Mature BDNF/proBDNF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF ( BEK-2211 ) and full-length proBDNF ( BEK-2217 ). Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate), cell lysates and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2211 (Mature BDNF Rapid TM ELISA) and BEK-2217 (proBDNF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. For human blood samples, we suggest the use of BEK-2241 (Mature BDNF/proBDNF Combo kit) . The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance. Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
See BEK-2211 & BEK-2217 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature BDNF and proBDNF in Culture Supernatant, Serum, Plasma (Citrate), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x BDNF antibody, 1 x proBDNF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Sharma GP et al. (2021) Brain-derived neurotrophic factor promotes immune reconstitution following radiation injury via activation of bone marrow mesenchymal stem cells. PLoS One. 16(10):e0259042 Application: Mouse, culture supernatant. Weaver KR et al. (2021) Neuronal-enriched extracellular vesicles in individuals with IBS: A pilot study of COMT and BDNF. Neurogastroenterol Motil. [Epub ahead of print] Application: Human, plasma, extracellular vesicles.
Specificity:
Mature BDNF ELISA: Human, mouse, rat BDNF and numerous other species. Although the BDNF assay antibodies are raised against the mature domain, the mature BDNF ELISA preferentially detects mature BDNF over full-length proBDNF. proBDNF ELISA: Human, mouse and rat proBDNF. The capture antibody used in the proBDNF ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF, and does not quantify mature BDNF.
Typical limit of detection (LOD) for mature BDNF is less than 2 pg/mL, and 10 pg/mL for proBDNF determined as 150% of the blank value.
Cross Reactivity:
<b>Cross-reactivity of BDNF isoforms:</b><br><br><b>Mature BDNF ELISA:</b><br>No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM).<br><br><b>proBDNF ELISA:</b><br>A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75 ECD ELISA kit employs a recombinant human p75 ECD -Fc chimera. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD . Note: For research use only. Not for diagnostic and clinical purposes. Receive a 20% discount on the Biosensis Creatinine (Urinary) Colorimetric Assay Kit (CRE-001) if purchased together with this ELISA kit in one transaction. To receive a quote, contact us at sales@biosensis.com .
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75 ECD ELISA kit employs a recombinant human p75 ECD -Fc chimera. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD . Note: For research use only. Not for diagnostic and clinical purposes. Receive a 20% discount on the Biosensis Creatinine (Urinary) Colorimetric Assay Kit (CRE-001) if purchased together with this ELISA kit in one transaction. To receive a quote, contact us at sales@biosensis.com .
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Mouse and Rat proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant mouse, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Mossa A et al. (2021). Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder. Exp Gerontol. [Epub ahead of print]. Application: Rat urine. Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). Mossa AH et al. (2020). Antagonism of proNGF or its receptor p75NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Specificity:
Mouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Typical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Reacts with human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
The Biosensis Mouse and Rat proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant mouse, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Mossa A et al. (2021). Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder. Exp Gerontol. [Epub ahead of print]. Application: Rat urine. Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). Mossa AH et al. (2020). Antagonism of proNGF or its receptor p75NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Specificity:
Mouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Rabbit anti-Adrenocorticotropic hormone (ACTH) Polyclonal Antibody (Unconjugated), suitable for IHC-Paraffin-embedded.
Background Info:
Adrenocorticotropic hormone (ACTH) is cleaved from the precursor pro-opiomelanocortin (POMC). The hormone is produced and secreted by the pituitary gland and stimulates release of cortisol by adrenal glands.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
Porcine Adrenocorticotropic hormone (ACTH) conjugated to BSA
Applications:
IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Corticotropin; ACTH; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat; ACTH is highly conserved so cross-reactivity with other species is expected.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain. Mutations in the TrkB gene have been associated with obesity and mood disorders.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IF (1:1000-1:3000), 1-site ELISA (1:10,000 dilution). A dilution of 1:1000 to 1:3000 o/n is recommended for IF. 4% PFA frozen sections tested. Not yet tested on paraffin embedded tissues. Use triton X-100 permeabilizaiton with frozen sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rudolph M (2020). Mesolimbic GluA1 AMPA Receptor Signaling in Dopaminergic Neurons Plays a Critical Role in the Induction of Cross-Sensitization to Psychostimulants in Response to Social Stress. PhD Thesis. Application: IHC (IF), frozen sections. Species: Rat. Penzo MA et al. (2015). The paraventricular thalamus controls a central amygdala fear circuit. Nature. [Epub ahead of print]. Application: IHC (IF), 4% PFA, frozen sections. Species: Mouse.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SPRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (aa: 32-47) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Immunohistochemistry (IHC):</b> 0.5-1 ug/mL. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals.<br><b>Western Blotting (WB):</b> 0.5-2 ug/mL. This antibody demonstrates clear immunoreactivity for TH at 60 kDa in rat PC12 cell lysate and mouse brain homogenate. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunohistochemistry on 4% PFA fixed or 2% PLP fixation, 1:100-1000 primary antibody dilution. IC, Immunocytochemistry: acetone fixed specimens recommended. 1:100-1:1000 primary antibody dilution. Western Blot: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol). 1:500 overnight 4 degrees is recommended for primary antibody dilution in western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC, WB and ELISA confirmed the specificity for MAP1LC3 A. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 10 (ATG10) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 10; APG10-like; ATG10
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG10. Human. Other species not yet tested.
Storage:
After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Gamma-aminobutyric acid receptor-associated protein (GABARAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
GABARAP is highly positively charged in its N-terminus and shares sequence homology with MAP1LC3 1A and 1B. This protein clusters neurotransmitter receptors (GABA(A) receptors) by mediating interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. TISSUE SPECIFICITY: Heart, brain, placenta, skeletal muscle, kidney and pancreas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (FEKRRSEGEKIC) corresponding to the N-terminal of human GABARAP protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC, WB and ELISA confirmed the specificity for GABARAP. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Gamma-aminobutyric acid receptor-associated protein (GABARAP)
FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family. ESTIMATED MOLECULAR WEIGHT: 13.667 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CVESAKIRAKYP) corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 3-related protein; GABARAPL2; FLC3A; GEF2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC, WB and ELISA confirmed the specificity for GABARAP L2 (GABARAPL2). A 14 kDa band, that corresponds to the molecular weight of GABARAPL2, is detected via western blot analysis. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beclin-1 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beclin-1 plays a central role in autophagy. It may also play a role in antiviral host defense. It is ubiquitously expressed and it localises to the cytoplasm, golgi apparatus membrane, and in dendrites and cell bodies of cerbellar Purkinje cells. The unprocessed precursor has a length of 450 amino acids and an estimated molecular weight of 51.89 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
C-terminal of human Beclin-1 conjugated to Blue Carrier Protein.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; APG 6; BECN1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specific for Beclin-1. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Glutaraldehyde cross-linked ubiquitin.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin. Human. Predicted to react with other mammalian tissues.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Peripherin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length Peripherin protein expressed in and purified from E.coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,00-1:2,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ise H. et al (2017) Elucidation of GlcNAc-binding properties of type III intermediate filament proteins, using GlcNAc-bearing polymers. Genes Cell. 2017 Sep; [Epub ahead of print] Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein and a smaller molecule derived from Peripherin at ~48 kDa. Hu, Rat, Ms, Fel, Rb. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C protein in Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC and WB confirmed the specificity for MAP1LC3 C . This antibody should recognise MAP1LC3 C only and not the other forms MAP1LC3A and MAP1LC3B. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C)
Rabbit anti-Neuron specific enolase (NSE) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neuron Specific Enolase (NSE) expressed in and purified from E.coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Neurofilament Medium (NF-M) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB, ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. Hu, Rat, Ms, Fel, Chk
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human NF-L protein
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:2,000 - 1:5,000 is recommended for ICC/IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted),Rat
Immunogen:
Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E.coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. This antibody stains dendritic and perikaryal neurofilaments particularly well. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with noth phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. Rat. Predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H)
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Native NF-H purified from bovine spinal cord
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunocytochemistry (IHC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for ICC/IHC is 1:1,000-1:5,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Hashiguchi S et al. (2019) "Ataxic phenotype with altered CaV3.1 channel property in a mouse model for spinocerebellar ataxia 42." Neurobiol Dis. Jun 20:104516 [Epub ahead of print]. Application: IHC. Species: Human.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa. Rat. Predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. MMP2 isoform 2 mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. Catalytic activity of MMP2 causes cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Western Blotting (WB): 1:5,000 - 1:10,000. MMP2 appears as two bands at apparent molecular weights of 66 and 72 kDa. Immunohistochemistry (IHC): 1:500-1:1,000.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
72 kDa gelatinase; Gelatinase A; MMP-2; TBE-1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human. Expected to react with horse, cow, pig, chicken, rat and mouse MMP2.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Myristoylated alanine rich C kinase substrate (MARCKS) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Myristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant full length MARCKS expressed and purified from E. coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein;Marcks; Macs;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. Does not react with rodent protein.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Myristoylated alanine rich C kinase substrate (MARCKS)
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Flow Cytometry (2 ?g/10^6 cells), IHC, immunofluorescence, WB. Use a dilution of 1:200 to 1:1000 for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Villanueva-Paz M. et al. (2016) Amitriptyline induces mitophagy that precedes apoptosis in human HepG2 cells. Genes Cancer. 2016;7(7-8):260-277 Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease Br J Pharmacol. 2012 Jul 2. De la Mata M. et al (2012) Recovery of MERRF fibroblasts and cybrids pathophysiology by Coenzyme Q10 Neurotherapeutics. 2012 Apr;9(2):446-63. Cotan D. et al. (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts FASEB J. 2011
Specificity:
IHC and wb confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Cat,Human,Mouse,Other Mammals,Rat
Immunogen:
Recombinant alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, Rat, Ms, Fel, and other mammals
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB, and 1:1,000 - 1:5,000 for ICC and IHC. A dilution of 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, immunofluorescence, WB (1-5 µg/mL working concentration), Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Coronin- 1A Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Coronins belong to the WD40 or WD family of proteins. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family). The mammalian coronin family members are abundant components of eukaryotic cells and each type has a restricted cell type specific expression pattern. Coronin 1A is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. This antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human coronin 1a coupled to KLH
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,500-5,000 is recommended for WB. Human Coronin 1A has a predicted length of 461 residues and a MW of 51 kDa. A concentration of 1:500-1:1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO; CORO1A; CORO1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Stephens A.N. et al (2010) Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE. J Proteome Res. 2010 May 7;9(5):2438-49. Ahmed Z. et al (2007) Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry. J Histochem Cytochem. 2007 Jul;55(7):687-700.
Specificity:
The specificity of this antibody has been confirmed by WB and ICC against the antigen. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
PERK (PKR-like ER kinase) is a single-pass type I ER membrane protein with a stress-sensing luminal domain connected by a transmembrane segment to a cytoplasmic-kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A recombinant peptide from mouse PERK.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:500 is recommended for WB. A dilution of 30 µL of antibody in a total reaction mixture of 500 µL is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feliziani C. et al (2022) Ca2+ signalling system initiated by Endoplasmic reticulum stress stimulates PERK activation Cell Calcium. 2022 [Epub ahead of print] Bollo M. et al (2010) Calcineurin interacts with PERK and dephosphorylates calnexin to relieve ER stress in mammals and frogs PLoS One. 2010 Aug 5;5(8)
Specificity:
This antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL2 is an isoform of APPL1 with 54% identity in their protein sequences. APPL2 has a role in cell proliferation and embryonic development. Recently, it was shown that APPL2 regulates FSH signaling and acts as a negative regulator in adiponectin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Mouse APPL2 protein (amino acids: 619-662) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution between 1:2500 and 1:5000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2; APPL2; DIP13B;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2)
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL1 (adaptor protein containing PH domain, PTB domain and leucine zipper motif 1) mediates adiponectin signaling in various types of cells. APPL1 is also thought to have a role in insulin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:1,000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-alpha; Dip13-alpha; Adapter protein containing PH domain; PTB domain and leucine zipper motif 1; APPL1; APPL; DIP13A; KIAA1428;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL1.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1)
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Human Adiponectin Receptor 2 protein (amino acids: 78-219) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Progestin and adipoQ receptor family member II; ADIPOR2; PAQR2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The intracellular portion of mouse Adiponectin Receptor 1 protein (amino acids: 4-142) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adipor1; AdipoR1; Progestin and adipoQ receptor family member I; ADIPOR1; PAQR1;TESBP1A; CGI-45;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 1.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Myc Tag Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
The Myc tag contains the amino acids Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu (E-Q-K-L-I-S-E-E-D-L) corresponding to amino acids 410-419 of human Myc. This tag is widely used for monitoring expression of recombinant proteins in bacteria, insect and mammalian cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
Myc epitope tag peptide
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
ELISA and Western Blotting (WB). Suggested dilutions for ELISA at 1:1,000 and WB 1:500 - 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Martel, D. et al. (2017) Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Res. Int. 2017 Article ID 4635605. Application: WB
Specificity:
This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Storage:
Maintain at -20°C prior to opening for up to 1 year. The product is also stable at 2-8°C undiluted for several weeks. After opening store at -20°C in undiluted aliquots for up to six months. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Avoid repeated freezing and thawing.
Rabbit anti-Maltose-Binding Protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Maltose binding protein (MBP) is encoded by the malE gene of E.coli. MBP is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
MBP epitope tag recombinant protein.
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and ELISA. Suggested starting dilutions for WB of 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.02% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
ELISA, WB, IP, IF, IHC. Western Blotting at a suggested concentration of 1:500~1:1000. ELISA at a suggested concentration of 1: 2500. IP 5 µg/sample. IF 5 µg/mL. IHC 5 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
His-tagged fusion proteins
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Rabbit anti-Influenza hemagglutin (HA) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Human influenza hemagglutin (HA) tag corresponds to a region (98-106 amino acids) from the HA molecule.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
The antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting at a suggested dilution 1:500~1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody detects HA-tagged proteins (on amino- and carboxy termini) in transfected mammalian cells.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Rabbit anti-Contactin-6 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3 subgroup member of immunoglobulin superfamily. It is expressed exclusively in the nervous system and mainly upregulated at the early postnatal stage during mouse brain development. Employing Northern blot analysis Kamei et al found that amongst different regions of the adult human nervous system cerebellum expressed highest level of NB-3 mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast, the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 like other neural recognition molecules plays a vitally important role in axonal guidance during development, plasticity, and maintenance of synaptic connections in the adult brain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate in oligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of the Notch intracellular domain and promotes oligodendrogliogenesis from progenitor cells and differentiation of oligodendrocyte precursor cells via Deltex1. In primary oligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence, the NB-3/Notch signaling pathway may be worthwhile a closer examination for its potential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-3 86% identity in nucleotide sequences and 90% identity in amino acid sequences. FUNCTION: Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum. DEVELOPMENTAL STAGE: Highly expressed after birth, reaching a maximum at the postnatal day 7, and declines thereafter in the cerebrum, whereas it increases in the cerebellum to adulthood.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (KLHKMSSLSSVGVQILKPSTQF) as part of rat contactin-6 conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1: 5000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neural recognition molecule NB-3; mNB-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes. Confirmed to react with rat Contactin-6
Storage:
After reconstitution keep aliquots at minus 20ºC for increased stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (ASGEPVV LSGQIT) as part of mouse superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Park KH et al. (2013) Postnatal muscle modification by myogenic factors modulates neuropathology and survival in an ALS mouse model. Nat Commun. 2013;4:2906.
Specificity:
Specificity was confirmed by western blot detecting mouse superoxide dismutase (SOD1). This antiserum is known to react with mouse and rat superoxide dismutase (SOD1) protein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was confirmed by western blot detecting human superoxide dismutase (SOD1). This antiserum is known to react with human superoxide dismutase (SOD1).
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Noxa Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A synthetic peptide (CAQLRR IGDKVNLRQK) as part of mouse Noxa (aa: 75-90) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PMAIP1; phorbol-12-myristate-13-acetate-induced protein 1; adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; PMA-induced protein 1; Pmaip1; Noxa
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum. This antiserum cross-reacts with mouse. Not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Noxa Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A synthetic peptide (MPGRKARRNA PVNPTR) as part of mouse Noxa (aa: 1-16) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PMAIP1; phorbol-12-myristate-13-acetate-induced protein 1; adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; PMA-induced protein 1; Pmaip1; Noxa
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum. This antiserum cross-reacts with mouse. Not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. For WB, a 1:500 to 1:1000 dilution is recommended. The sequence of the immunogen and the conjugate are identical to the one used by Yan et al (see the Refs) and the antiserum appears to have similar characteristics. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Warren PM et al. (2018) "Rapid and robust restoration of breathing long after spinal cord injury." Nat Commun. 2018 Nov. PMID: 30482901 9(1):4843. Application: IHC/IF. Species: Rat, spinal cord sections. Wang T et al. (2016) "Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB." Nat Commun. 2016 Sep. PMID: 27687129 30(7):12976. Application: IF & structured illumination microscopy (SIM). Species: Rat, Mouse; fixed 4% PFA with 4% sucrose Matusica D et al. (2016) Inhibition of motor neuron death in vitro and in vivo by a p75 neurotrophin receptor intracellular domain fragment. J Cell Sci. 2016 Feb 1;129(3):517-30. Application: WB. Brock JH et al. (2010) Local and remote growth factor effects after primate spinal cord injury. J Neurosci. 2010 Jul 21;30(29):9728-37. Gruber H. et al. (2008) Brain-derived neurotrophic factor and its receptor in the human and the sand rat intervertebral disc. Arthritis Res Ther. 2008; 10(4): R82.
Specificity:
Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: This is a specific receptor for parathyroid hormone. The activity of this receptor is mediated by g proteins which activate adenylyl cyclase. PTHR2 may be responsible for PTH effects in a number of physiological systems. It may play a significant role in pancreatic function. PTHR2 presence in neurons indicates that it may function as a neurotransmitter receptor. SUBUNIT: Binds to TIPF39/TIP39. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in brain, arterial and cardiac endothelium. Found as well in sperm, in the head of the epididymis. Lower expression is found in vascular smooth muscle, exocrine pancreas, testis and placenta. SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (RQIDSHVTLPGYVWSSSEQDC) of rat Parathyroid hormone receptor protein (aa: 481-501) conjugated to KLH has been used as the immunogen.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity has been shown by western blot. A band of 63 kDa corresponding to the theoretical molecular weight of Parathyroid hormone receptor is easily detectable by WB. Absorption of the antiserum with the immunising peptide abolished the binding of the antibody to the target hence detectability of the band. This antibody is known to react with rat Parathyroid hormone receptor.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Rat
Immunogen:
A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
<b>Immunohistochemistry (IHC):</b> 1:2,000 to 1:5,000, dilutions of up to 1:100,000 have been reported. This is a superb antibody for detection of tyrosine hydroxylase (TH) containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. <br><b>Western blotting (WB)</b>: 1:100 to 1:500. Antibody has been tested on RIPA-extracted PC12 cell lysate and shown to be specific for TH (~60 kDa). Tissue homogenates show a higher level of non-specific binding and presence of uncharacterized bands. Affinity-purified anti-TH antibody (R-148-50) is recommended for tissue homogenates. <br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Pierre S.R., Lemmens M.A., Figueiredo-Pereira M.E. (2009) Subchronic infusion of the product of inflammation prostaglandin J2 models sporadic Parkinson's disease in mice J Neuroinflammation. Jul 25;6:18 Takeoka A. et al (2010) Noradrenergic innervation of the rat spinal cord caudal to a complete spinal cord transection: effects of olfactory ensheathing glia J Exp Neurol. 2010 Mar;222(1):59-69. Brown R.E. et al (2008) Characterization of GABAergic neurons in rapid-eye-movement sleep controlling regions of the brainstem reticular formation in GAD67-green fluorescent protein knock-in mice. Eur J Neurosci. 2008 Jan;27(2):352-63. Bisem NJ et al (2012) Mapping of FGF1 in the Medulla Oblongata of Macaca fascicularis. Acta Histochem Cytochem. 2012 Dec 26;45(6):325-34.
Specificity:
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Synphilin-1 (Sph1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CASKGKNKAA) as part of human synphilin-1a conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1: 1000 to 1:2000 is recommended for immunohistochemistry and 1:500-1:1000 for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A high level of specificity has been shown for this antiserum by IHC on human brain. This antiserum is known to react with rat and human synphilin-1a.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Synphilin-1 (Sph1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SLELNGEKDKDKGRTLQRT) as part of human synphilin-1a conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1: 1000 to 1:2000 is recommended for this application. This antiserum will stain Lewy bodies in Dorsal raphe nucleus of Parkinson's diseased human brain. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A high level of specificity has been shown for this antiserum by IHC on human brain. This antiserum is known to react with rat and human synphilin-1a.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for immunohistochemistry and 1:2000 to 1:4000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Parkinson disease protein 2 (Parkin) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (NSLIKELHHFRILGEEQ) as part of human Parkin conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 is recommended for immunohistochemistry and 1:2000 for western blot. Nice staining is achieved in neuronal and cytoplasmic granules sections treated with citrate buffer for antigen retrieval. Few inclusions are stained but these were not positively identified as Lewy bodies. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Song Y. J. C. et al (2009). Degeneration in different parkinsonian syndromes relates to astrocyte type and astrocyte protein expression. J. Neuropathol. Exp. Neurol. Oct 2009;68(10):1073-1083 Huang Y. et al (2008). LRRK2 and parkin immunoreactivity in multiple system atrophy inclusions. Acta Neuropathol. 2008 Dec;116(6):639-46.
Specificity:
This antiserum is known to be highly specific for Parkin shown by IHC and WB. This antibody is known to react with rat and human Parkin.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Parkinson disease protein 2 (Parkin) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (RILGEEQYNRYQQYGAEEC) as part of human Parkin conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, immunoblot, 1-site ELISA. A dilution of 1:500 to 1:2000 is recommended for these applications. This antiserum stains trigeminal motor neurons in rat brain stem. A 50 kDa band was identified in rat brain extract using western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Francelin C et al (2021) BACE1 Inhibition Increases Susceptibility to Oxidative Stress by Promoting Mitochondrial Damage. Antioxidants (Basel). 10(10):1539. E. Rubio de la Torre et al (2009) Combined kinase inhibition modulates parkin inactivation. Hum Mol Genet. 2009 Mar 1;18(5):809-23. D'Agata V. et al (2009) Parkin expression profile in dopamine d3 receptor knock-out mice brains. Neurochem Res. 2009 Feb;34(2):327-32. Tamo W. et al (2007) Parkin is expressed in vascular endothelial cells. Neurosci Lett. 2007 Jun 4;419(3):199-201. Trimmer P.A. et al (2004) Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies. J Neurochem. 2004 Feb;88(4):800-12. Denison S.R. et al (2004) Alterations in the common fragile site gene Parkin in ovarian and other cancers. Oncogene. 2003 Nov 13;22(51):8370-8. Pawlyk A.C. et al (2003) Novel monoclonal antibodies demonstrate biochemical variation of brain parkin with age. J Biol Chem. 2003 Nov 28;278(48):48120-8. Horowitz J.M. et al (2001) Spatial distribution, cellular integration and stage development of Parkin protein in Xenopus brain. Brain Res Dev Brain Res. 2001 Jan 31;126(1):31-41.
Specificity:
This antiserum is known to specifically recognise Parkin shown by IHC and WB. This antibody is known to react with Parkin of guinea pig and rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; APG 12; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; Apoptosis-specific protein; APG5; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
E.N. Wilson et al (2011) A Switch Between Cytoprotective and Cytotoxic Autophagy in the Radiosensitization of Breast Tumor Cells by Chloroquine and Vitamin D. Horm Cancer. 2011 Sep 2. Bristol ML et al (2012) Dual functions of autophagy in the response of breast tumor cells to radiation: cytoprotective autophagy with radiation alone and cytotoxic autophagy in radiosensitization by vitamin D 3. Autophagy. 2012 May 1;8(5):739-53. Guido C et al (2012) Metabolic reprogramming of cancer-associated fibroblasts by TGF-? drives tumor growth: connecting TGF-_ signaling with "Warburg-like" cancer metabolism and L-lactate production. Cell Cycle. 2012 Aug 15;11(16):3019-35.
Specificity:
IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Guinea Pig,Human,Rat
Immunogen:
Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), Western Blot, radioimmunoassay and inhibition of enzymatic activity. Recommended to be used at a dilution of 1:500 to 1: 2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Bowolaksono A. (2012) Molecular Regulation of Noradrenaline in Bovine Corpus Luteum. HAYATI Journal of Biosciences June 2012 Vol. 19 No. 2, p 81-87 Blanco-Centurion C. et al (2004) Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci. 2004 May;19(10):2741-52. Ohshita N. et al (2004) Characteristics of the trigeminal depressor response in cats. J Neurosci Res. 2004 Jun 15;76(6):891-901.
Specificity:
This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Contactin-6 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3 subgroup member of immunoglobulin superfamily. It is expressed exclusively in the nervous system and mainly upregulated at the early postnatal stage during mouse brain development. Employing Northern blot analysis Kamei et al found that amongst different regions of the adult human nervous system cerebellum expressed highest level of NB-3 mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast, the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 like other neural recognition molecules plays a vitally important role in axonal guidance during development, plasticity, and maintenance of synaptic connections in the adult brain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate in oligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of the Notch intracellular domain and promotes oligodendrogliogenesis from progenitor cells and differentiation of oligodendrocyte precursor cells via Deltex1. In primary oligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence, the NB-3/Notch signaling pathway may be worthwhile a closer examination for its potential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-3 86% identity in nucleotide sequences and 90% identity in amino acid sequences. FUNCTION: Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (GYPSPHYRWKQ) as part of mouse contactin-6 conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neural recognition molecule NB-3; mNB-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes. Confirmed to react with rat Contactin-6 and expected to recognise human and mouse Contactin-6 as well.
Storage:
After reconstitution keep aliquots at -20°C for increased stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Nociceptin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
FUNCTION: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. PTM: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. SIMILARITY: Belongs to the opioid neuropeptide precursor family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Rat
Immunogen:
A synthetic peptide (C-TG ARKSARKLAN Q) as part of rat Nociceptin peptide (aa: 139-151) conjugated to diphtheria toxoid
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA. A dilution of 1: 1000 to 1: 3000 is recommended for both applications. This antibody may also be used for staining of nerve fibres in guinea pig myenteric plexus. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
There are no synonyms for this peptide; however the precursor protein contains: Neuropeptide 1; Nociceptin (Orphanin FQ; PPNOC; ORL1 receptor agonist); Neuropeptide 2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been tested by the use of different peptides including enkephalin, dynorphin and endorphin for absorption in immunihistochemistry. This antibody is known to cross react with guinea pig and rat.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Orexin-A Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Rat
Immunogen:
A synthetic peptide (CRLYELLHGAGNHAAGILTL) as part of Bovine Orexin A (aa: 14-33) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. This is a superb antiserum for immunohistochemistry on Orexin A containing neurons exhibiting intense labelling of neurons with very low back ground. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Orexin-A; Hypocretin-1; Hcrt1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kruger J.L. et al (2010) Cellular location and major terminal networks of the orexinergic system in the brains of five microchiropteran species. J Chem Neuroanat. 2010 Nov;40(3):256-62. Gaykema R.P. et al (2009) Lipopolysaccharide challenge-induced suppression of Fos in hypothalamic orexin neurons: their potential role in sickness behavior. Brain Behav Immun. 2009 Oct;23(7):926-30. Lee H.S. et al (2005) Retrograde study of hypocretin-1 (orexin-A) projections to subdivisions of the dorsal raphe nucleus in the rat. Brain Res. 2005 Oct 12;1059(1):35-45. Yao S.T. et al (2005) Water deprivation increases the expression of neuronal nitric oxide synthase (nNOS) but not orexin-A in the lateral hypothalamic area of the rat. J Comp Neurol. 2005 Sep 19;490(2):180-93.
Specificity:
The specificity for this antiserum has been confirmed by immunohistochemistry on rat brain and the results reflect the current literature. This antibody is known to react with rat Orexin A.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Neurokinin-3 Receptor (NK-3R) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
FUNCTION: This is a receptor for the tachykinin neuropeptide neuromedin K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. PTM: The anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue. MISCELLANEOUS: The rank order of affinity of this receptor to tachykinins is: neuromedin K > substance K > substance P. SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (ASTTSSF ISSPYTSVDE YS) corresponding to the absolute C-terminal of rat NK-3 receptor protein (aa: 434-452) conjugated to KLH
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neuromedin K receptor; NKR; Neurokinin B receptor; NK-3 receptor; NK-3R; Tachykinin receptor 3; Tacr3; Tac3r
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been shown by western blot using rat brain homogenate. A band of 66 kDa, the theoretical MW of NK-3R, could be easily detected. This antiserum is know to cross react with rat NK-3 R.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, ICC, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 1-10 µg/mL for immunohistochemistry, ELISA, ICC and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human CNTF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1: 4000 is recommended for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody specifically detects CNTF shown by western blot. This antiserum to known to react with rat, mouse and human CNTF protein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS with no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:1000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 2-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 without preservatives
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Tumor necrosis factor receptor superfamily member 19 (TROY) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Can mediate activation of c-Jun and NF-kappa-B. May promote caspase-independent cell death. Isoform 2 and isoform 3 may act as decoy receptors. SUBUNIT: Associates with TRAF1, TRAF2, TRAF3 and TRAF5. SUBCELLULAR LOCATION: Isoform 1, isoform 3, isoform 4: Cell membrane; single-pass type I membrane protein (Probable). Isoform 2: Secreted protein (Probable). ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Highly expressed in adult brain, and in embryos from day 11-17, but not earlier. Detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, mainly in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. Isoform 3 was found in embryonic day 17.5 skin but not in brain and liver. SIMILARITY: Contains 3 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (CRPHRF KEDWGFQK) as part of mouse TROY protein (aa: 75-88) conjugated to the immunogenic protein Blue Carrier Protein
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Tumor necrosis factor receptor superfamily member 19; TNFRSF19; Toxicity and JNK inducer; TRADE
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for TROY was confirmed by IHC. This antiserum is known to react with rat TROY. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Tumor necrosis factor receptor superfamily member 19 (TROY)
Rabbit anti-Huntingtin-associated protein 1 (HAP-1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: In the brain, especially in the olfactory bulb and in the brain stem. No detectable expression in peripheral tissues such as lung, testis, spleen, and small intestine. SIMILARITY: Contains 1 HAP1 N-terminal domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
Recombinant rat HAP-1
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Huntingtin-associated protein 1; HAP1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for HAP-1 was confirmed by IHC. This antiserum is known to react with rat HAP-1. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
Extra cellular domain of human p75NTR
Applications:
ICC,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. Recommended to be used at a dilution of 1:500 to 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC shows specific staining for p75NTR. This antibody is known to react to rat p75NTR.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. <br><br>Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
L.Y. Chen et al (2010) Learning induces neurotrophin signaling at hippocampal synapses Proc Natl Acad Sci USA. Apr 13;107(15):7030-5 Soderquist R.G. et al (2009) PEGylation of brain-derived neurotrophic factor for preserved biological activity and enhanced spinal cord distribution J Biomed Mater Res A. 2009 Dec;91(3):719-29. Tang S. et al (2010) Immunolocalization of pro- and mature-brain derived neurotrophic factor (BDNF) and receptor TrkB in the human brainstem and hippocampus. Brain Res. Oct 1;1354:1-14. Sadri-Vakili G. et al (2010) Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. J Neurosci. 2010 Sep 1;30(35):11735-44. Maldonado M.A. et al (2008) Motor skill training, but not voluntary exercise, improves skilled reaching after unilateral ischemic lesions of the sensorimotor cortex in rats. Neurorehabil Neural Repair. 2008 May-Jun;22(3):250-61. Nakajima H. et al (2007) Rescue of rat anterior horn neurons after spinal cord injury by retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene. J Neurotrauma. 2007 Apr;24(4):703-12. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains. J Histochem Cytochem. 2007 Jan;55(1):1-19. Carrasco M.A. et al (2007) Regulation of glycinergic and GABAergic synaptogenesis by brain-derived neurotrophic factor in developing spinal neurons. Neuroscience. 2007 Mar 16;145(2):484-94. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:5000 is recommended for both applications. ICC: 1:500 to 1:2000, antibody works on 4% formaldehyde fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antiserum is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Mulhall J.P. et al (2008) J Sex Med. May;5(5):1126-36.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
<b>Western Blotting:</b> This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014).<br><br><b>ELISA:</b> Detection only, 1:1000-1:5000 recommended.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
McLean NA, Popescu BF, Gordon T, Zochodne DW, Verge VM. (2014) "Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves." PLoS One. 2014 Oct 13;9(10):e110174 Application: WB , non-reducing, Species: Rat Cysneiros R.M. et al (2010) Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids Epilepsy Behav. 2010 Jan;17(1):33-8. Ooe N. et al (2009) Dynamic regulation of bHLH-PAS-type transcription factor NXF gene expression and neurotrophin dependent induction of the transcriptional control activity Biochem Biophys Res Commun. 2009 Jan 23;378(4):761-5.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, mouse and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. <br />FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. <br />DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB and dot blot. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. The predicted molecular weight of the GDNF monomer is 11.6 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in Western Blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, Western blot. Recommended to be used at a dilution of 1: 1000 to 1: 2000 for immunohistochemistry and Western blot, for Immunofluorescence at a dilution of 1:50 to 1: 200 in free-floating sections or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, containing 0.02% sodium azide as preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, Immunofluorescence, Western blot and Immunoblot. Recommended to be used at a concentration of 0.5-1 µg for these applications. This antiserum works superbly for immunohistochemistry on free-floating or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Jabin Fagelskiold A et al (2012) Insulin-secreting INS-1E cells express functional TRPV1 channels. Islets. 2012 Jan 1;4(1).
Specificity:
Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord. Human, rat. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (SEEGYQDYEPEA) corresponding to the C-terminal of human alpha synuclein protein (aa 129-140) conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from alpha synuclein protein in monkey and pig.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:3000 is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Tinsley R.B. et al (2010) Sensitive and specific detection of alpha-synuclein in human plasma J Neurosci Res. 2010 Sep;88(12):2693-700.
Specificity:
Immunohistochemical and western blot analysis of human brain indicates a high level of specificity for this antiserum. This antibody is known to react with human, mouse and rat alpha synuclein. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Adenosine triphosphate (ATP)- ase (ATPase) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (C-ELHRQEEAKQVLRYY) as part of mouse ATP13A2 protein (aa: 147-161) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry shows specific staining for ATPase. This antiserum is known to react with rat ATPase. Other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Adenosine triphosphate (ATP)- ase (ATPase) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-DDVHRSRHGLSLQDQ) as part of human ATP13A2 protein (aa: 195-209) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry shows specific staining for ATPase. This antiserum is known to react with rat ATPase. Other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Amyloid-beta precursor protein (APP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity). FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1 inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues: kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (HMNVQNGKWESDPSGTKTC, aa: 44-62) as part of mouse APP isoform A conjugated to the immunogenic protein Blue Carrier Protein
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:3000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Amyloid beta A4 protein; ABPP; Alzheimer disease amyloid protein homolog; Amyloidogenic glycoprotein; AG
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for APP was confirmed by IHC. This antiserum is known to react with rat APP. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Amyloid-beta precursor protein (APP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity). FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1 inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues: kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
Synthetic peptides (C-ETHLHW HTVAKET, aa: 145-157; C-HAH FQKAKERLEA KHRER, aa: 388-405; C-KKKQYTS IHHGVVE, aa: 724-737) as parts of human APP isoform A conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:3000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity for APP was confirmed by IHC. This antiserum is known to react with rat APP. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity). SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). SIMILARITY: Belongs to the GDNFR family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (MGLSWSPRPPL) as part of mouse GFR alpha-3 protein (aa: 1-11) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains nicely large neurons in the rat DRG. Specifically, if you would like to perform WB using rat brain homogenate detecting GFR alpha-3, this is the antibody to be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
GDNF family receptor alpha-3; GFR-alpha-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis indicates a high level of specificity to GFR alpha-3 for this antiserum. This antibody is known to react with rat GFR alpha-2.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PRVEKTPSLPDDLSD) as a part of human GFR alpha-2 protein (aa: 376-390) conjugated to KLH.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains beautifully the large neurons in the rat DRG. This antibody also is ideal for WB analysis of rat DRG and brain homogenate. The optimal dilution should be determined by the end user, but a dilution of 1 in 500 is recommended for initial testing. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Western blot analysis indicates a high level of specificity to GFR alpha-2 for this antiserum. This antibody is known to react with rat GFR alpha-2 and from IHC analysis, there appears to be no cross-reactivity with other members of the GFR family.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer, and Parkinson disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain.<br />FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting:</b> A concentration of 1-10 µg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate).<br><br><b>IHC:</b> Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. <b>ELISA:</b> 1-10 µg/mL capture/detection.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Detects human alpha-, beta-, and gamma synuclein proteins. A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins conjugated to diphteria toxoid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and immunoblot. A dilution of 1:1000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ulusoy A. et al (2010) Eur J Neurosci. 2010 Aug;32(3):409-22 Naesstrom T. et al (2010) The lipid peroxidation products 4-oxo-2-nonenal and 4-hydroxy-2-nonenal promote the formation of _-synuclein oligomers with distinct biochemical, morphological, and functional properties. Free Radic Biol Med. 2010 Dec 1. Eslamboli A. et al (2007) Long-term consequences of human alpha-synuclein overexpression in the primate ventral midbrain. Brain. 2007 Mar;130(Pt 3):799-815. Mukaetova-Ladinska E.B. et al (2008) Alpha- and gamma-synuclein proteins are present in cerebrospinal fluid and are increased in aged subjects with neurodegenerative and vascular changes. Dement Geriatr Cogn Disord. 2008;26(1):32-42.
Specificity:
Overlap specific immunohistochemical staining of alpha-, beta- and gamma synucleins This antiserum recognises human and rat alpha-, beta- and gamma synucleins.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Gamma-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. SIMILARITY: Belongs to the synuclein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (aa: 114-127) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical/western blot analysis indicate a high level of specificity for this antiserum for gamma synuclein. This antiserum is known to react with human and rat gamma synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB , immunoblot. A dilution of 1:500 to 1:2000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Israeli E. and Sharon R. (2009) Beta-synuclein occurs in vivo in lipid-associated oligomers and forms hetero-oligomers with alpha-synuclein J Neurochem. 2009 Jan;108(2):465-74
Specificity:
Less than 0.1% cross reactivity to human alpha synuclein This antiserum is known to react with human and rat beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (YFSHLKEYVAS) of human capsaicin receptor protein (aa: 531-541) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rehman R et al (2013) TRPV1 inhibition attenuates IL-13 mediated asthma features in mice by reducing airway epithelial injury. Int Immunopharmacol. 2013 Mar;15(3):597-605.
Specificity:
Immunohistochemical analysis of rat DRG indicates a superb degree of specificity for this serum. This antibody is known to react with rat Capsaicin and due to sequence homology is expected to react with mouse Capsaicin . Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Pig,Rabbit,Rat
Immunogen:
A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times<br>ELISA: direct antigen ELISA<br>This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beig MI, Dampney BW and Carrive P (2014) Both ox1r and ox2r orexin receptors contribute to the cardiovascular and locomotor components of the novelty stress response in the rat. Neuropharmacology September 16 [Epub ahead of print] Application: IH ; Species: Rat Ellis KM, O'Carroll DC, Lewis MD, Rychkov GY, Koblar SA (2014) Neurogenic potential of dental pulp stem cells isolated from murine incisors. Stem Cell Res Ther. 2014 Feb 27;5(1):30. Application: IF ; Species: Mouse Leong WK, Klaric TS, Lin Y, Lewis MD, Koblar SA (2013) Upregulation of the neuronal Per-Arnt-Sim domain protein 4 (Npas4) in the rat corticolimbic system following focal cerebral ischemia. Eur J Neurosci. 2013 Jun;37(11):1875-84 Application: IH ; Species: Rat Li S et al (2011) The expression and localization of Prune2 mRNA in the central nervous system. Neurosci Lett. Oct 10;503(3):208-14.
Specificity:
This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Saporin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Plant
Immunogen:
Saporin, whole molecule
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot. A dilution of 1:200 to 1: 2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Saponaria officinalis; Common soapwort
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 µg/mL for ELISA and for inhibition of biological activity in vitro 1-10 µg/mL. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 µg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Serra, M.P. et al. (2022) Anti-Inflammatory Effect of Beta-Caryophyllene Mediated by the Involvement of TRPV1, BDNF and trkB in the Rat Cerebral Cortex after Hypoperfusion/Reperfusion Int. J. Mol. Sci. Mar; 23(7),3633 Lin J.C-Y. and Rosenthal A. (2011) Methods for treating obesity by administering a trkB antagonist US Patent US 7935342 B2 Counts S.E. and Mufson E.J. (2010) Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity J Neurochem. 2010 May;113(3):649-60. Unsain N. et al (2009) Brain-derived neurotrophic factor facilitates TrkB down-regulation and neuronal injury after status epilepticus in the rat hippocampus J Neurochem. 2009 Oct;111(2):428-40 Salehi Z., Mashayekhi F. (2009) Brain-derived neurotrophic factor concentrations in the cerebrospinal fluid of patients with Parkinson's disease J Clin Neurosci. Jan;16(1):90-3.
Specificity:
Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot. This antiserum is known to react with BDNF from rat, mouse and human. Expected to react with BDNF of other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
PE-1832 is A2 beta casein protein that is purified in its native form from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A2 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A2 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
PE-1831 is A1 beta casein protein that is in its native form and purified from bovine A1/A1 commercial cow's milk collected from cows that are certified BSE and disease free by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A1 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A1 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
Purification:
The product is >95% A1 beta casein based on SDS-PAGE gel. Affinity-purified, native protein
This peptide may be used to block binding activity of antibody to ChAT (# R-043-100 and # R-1621-100).
Product Type:
Peptide
Format:
Lyophilized.
Applications:
Block
Application Details:
Control peptide. This peptide may be used to block binding activity of antibody to ChAT (# R-043-100 and # R-1621-100). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Store lyophilized peptide dry at -20°C to -80°C. Store reconstituted peptide at 2-8°C for maximum of 2 days to avoid degradation. Aliquot the reconstituted peptide and store at -20°C to -80°C; avoid repetitive freeze-thaw cycles.
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al. , 2012 , followed by lyophilisation using Biosensis' proprietary stabilization procedures. The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric A? 1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assays, as well as other research applications.
Product Type:
Peptide
Format:
Lyophilized, Supplied as 2 x 500 ng vials, each containing lyophilized A? oligomers</b>. Note that the amount of provided oligomeric protein is based on the amount of monomeric A? used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
Applications:
ELISA
Application Details:
<i>Use as positive control in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.<br><br><i>Use as oligomeric A-beta peptide standard in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1750-1000_Instructions for Generating a Calibration Curve.pdf"> here </a> for detailed instructions on generating a calibration curve with PE-1750-1000.<br><br><i>Use as positive control in other applications</i>: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 - 200 µL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.
Store unopened, lyophilized oligomeric A? with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2-8°C for up to 2 days. The reconstituted material should not be frozen for best results.
Synthetic beta-amyloid A? 1-42 was monomerized by HFIP (hexafluoro-2-propanol) treatment and dried. One vial contains 50 ?g monomeric A? peptide that can be used to form solutions of unaggregated A? monomers, aggregated A? oligomers, A? fibrils and A? protein complexes according to published protocols, and used in a variety of research applications.
Product Type:
Peptide
Format:
Lyophilized.
Applications:
ELISA
Application Details:
<i>Preparation of unaggregated A-beta<sub>1-42</sub></i>:<br><br><b>Important:</b> unaggregated A-beta has to be prepared just prior to use!<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important</b>: refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM. Vortex-spin for 3 more times<br> 4. Final concentration of A-beta is 450 µg/mL<br>5. Use reconstituted peptide <b>immediately</b> to avoid oligomer formation<br><br><i>Preparation of oligomeric A-beta<sub>1-42</sub></i>:<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important</b>: refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM<br>4. Vortex-spin for 3 more times<br>5. Incubate the solution at 2-8ºC for 24 hours (protected from light)<br>6. Final concentration of A-beta is 450 µg/mL<br>7. Once reconstituted and oligomerized, o-A-beta should be used as soon as possible and within 7 days to ensure the stability of the oligomers<br><br><b>Note:</b> while the concentration of monomeric A-beta peptide used to form the oligomeric complexes is accurately determined, the precise formation, size and number of oligomers cannot be quantified by any known method.<br><br><i>Preparation of fibrillar A-beta<sub>1-42</sub></i>:<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important:</b> refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of 10 mM HCl to make up to 111 µL total volume and a peptide concentration of 100 µM<br>4. Vortex-spin for 3 more times<br> 5. Incubate the solution at 37ºC for 24 hours (protected from light)<br>6. Final concentration of A-beta is 450 µg/mL<br><br><i>Preparation of A-beta<sub>1-42</sub> Complexes</i>:<br><br><b>Important:</b> only unagreggated A-beta will form complexes. Use A-beta peptide immediately after reconstitution to form complexes.<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important:</b> refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM<br> 4. Vortex-spin for 3 more times<br> 5. Use reconstituted peptide <b>immediately</b> to avoid oligomer formation<br>6. Mix the A-beta monomer with its complex partner (eg., lipoprotein) at desired concentrations in PBS, pH 7.4, or other suitable buffers compatible with its intended application<br>7. Incubate at room temperature for 2 hours without shaking<br>8. Use complexes immediately after incubation<br><br>These protocols are based on procedures published by <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/22423893">Youmans KL <i>et al.</i>, 2012</a> and <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/23293020">Tai LM <i>et al.</i>, 2013</a>, and we refer to these publications and other relevant literature for further details.<br><br>Provided working concentrations are only meant to guide the user. Optimal concentrations depend on the experimental design and need to be determined empirically.
Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity all members of the neurotrophin family including beta NGF, BDNF, NT-3 and NT-4/5. It also binds the pro-neurotrophins. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The p75NTR NGF receptor is a type I transmembrane glycoprotein (396 aa) consisting of a signal peptide (21 aa), an extracellular domain (225 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (19 aa) and a cytoplasmic domain (152 aa). It is a member of the TNF-alpha receptor family (TNR16). Recently, p75NTR has been shown to act as a receptor for many pathogens such as prions, rabies virus and amyloid beta. It also acts as a co-receptor for NOGO, mediating inhibitory signals of myelin associated protein.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Background Info:
Leucine-Rich Repeat Kinase 2 (LRRK2) is a member of the leucine-rich repeat kinase family. Mutations in the LRRK2 gene have been linked with late-onset autosomal dominant Parkinson's disease (PD). A recent screening identified the analog LRRK2-IN-1 as a potent and selective inhibitor of LRRK2. This research by Deng et al (2011, Nat Chem Biol) showed that LRRK2-IN-1 suppressed LRRK2 kinase activity in vivo leading to dephosphorylation of Ser910/Ser935, loss of 14-3-3 binding and accumulation of LRRK2 within aggregrate fibrillar structures. LRRK2-IN-1, is the first selective and potent inhibitor of LRRK2. LRRK2-IN-1 inhibits both wild-type and G2019S mutant LRRK2 kinase activity with IC50 values of 13nM and 6nM respectively (Deng et al 2011, Nat Chem Biol).
Product Type:
Small Molecule
Format:
Dry, Synthetic chemical powder
Applications:
Block
Application Details:
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Deng X. et al (2011) Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2 Nat Chem Biol. 2011 Apr;7(4):203-5.
Storage:
Store dry, unopened vial protected from light for up to 3 months at RT or at -70ºC for up to 1 year. Reconstitute product in 100% ACS grade DMSO and store at -80ºC, in aliquots, protected from light, for up to 1 month. Prevent multiple freeze-thaw cycles. CAS Number 1234480-84-2.
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3GF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of rat NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kim GB et al. (2018) "The critical chemical and mechanical regulation of folic acid on neural engineering." Biomaterials. [Epub ahead of print] Application: Rat Schwann cell culture supernatants.
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of mouse NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kawanokuchi J et al. (2021) Acupuncture Treatment for Social Defeat Stress. Front Behav Neurosci. 15:685433. Application: Brain homogenate. Takagishi S et al. (2021) Protein nanoparticles modified with PDGF-B as a novel therapy after acute cerebral infarction. eNeuro. [Epub ahead of print]. Application: Brain homogenate. Leitao L et al. (2020) Osteoblasts are inherently programmed to repel sensory innervation. Bone Res. 8:20. Application: Culture supernatant. Yamada K et al. (2020) The impact of ovariectomy on olfactory neuron regeneration in mice. Chem Senses. [Epub ahead of print]. Application: Mouse olfactory bulb homogenate. Hutson TH et al. (2019) Cbp-dependent histone acetylation mediates axon regeneration induced by environmental enrichment in rodent spinal cord injury models. Sci Transl Med. 11(487). Application: Rodent DRG lysates. Miura-Yura E et al. (2019) Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin-induced diabetic mice. J Diabetes Investig. [Epub ahead of print]. Application: Conditioned medium of stem cells from human exfoliated deciduous teeth. Noda T et al. (2019) Effects of Tokishakuyakusan on regeneration of murine olfactory neurons in vivo and in vitro. Chem Senses. [Epub ahead of print]. Application: Mouse olfactory bulb homogenate.
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
Recombinant rat GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. Castro SL et al. (2022) Blueberry Juice Augments Exercise-Induced Neuroprotection in a Parkinsons Disease Model Through Modulation of GDNF Levels. IBRO Neurosci Rep. [Epub ahead of print] Application: Rat, brain tissue homogenate.
Specificity:
Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
Recombinant rat GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. Castro SL et al. (2022) Blueberry Juice Augments Exercise-Induced Neuroprotection in a Parkinsons Disease Model Through Modulation of GDNF Levels. IBRO Neurosci Rep. [Epub ahead of print] Application: Rat, brain tissue homogenate.
Specificity:
Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Mouse GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is less than 5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Mouse GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is less than 5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis Rat Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2214) and full-length proNGF (BEK-2236). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants and tissue homogenates only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2214 (Mature BDNF Rapid TM ELISA) and BEK-2236 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a high-quality recombinant rat NGF protein as standard, and thus the Mature NGF/proNGF Combo kit is designed to measure the rat NGF isoforms. However, the rat mature NGF ELISA kit will cross-react with mouse and human mature NGF protein. The proNGF ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli. Mouse proNGF and rat proNGF share 96% sequence homology, and internal validation data has demonstrated the ability of the proNGF ELISA to detect proNGF in rat PC12 cell lysates and rat brain tissue homogenate. However, the proNGF ELISA shows only little cross-reactivity (20%) with human proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates that the mature NGF ELISA assay antibodies preferentially detect the mature form of NGF. Cross-reaction of full-length proNGF was This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
See BEK-2214 & BEK-2236 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2214 (Rat Mature NGF Rapid TM ELISA Kit) and BEK-2236 (Mouse/Rat proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects rat NGF, and cross-reacts with human and mouse mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects mouse and rat proNGF only, and shows only little reactivity (20%) with human proNGF. The proNGF ELISA detects the full-length form of proNGF only, and does not quantify mature NGF or the pro-domain peptide.
Typical limit of detection (LOD) for rat mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for rat proNGF is less than 50 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the mature NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings.<br><br><b>proNGF ELISA:</b><br>Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of human NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum, plasma (EDTA and citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human serum and plasma samples. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Lamb WDB (2021) "Potential of Muller glia-derived extracellular vesicles for retinal neuroprotection." PhD Thesis Application: Human cell culture supernatant. Elisa A et al. (2021) "Aging is associated with cardiac autonomic nerve fiber depletion and reduced cardiac and circulating BDNF levels." J Geriatr Cardiol. 18(7): 549559 Application: Rat serum. Woo JH et al. (2019) "Effect of Dehydrocostus Lactone Isolated from the Roots of Aucklandia lappa on the Apoptosis of Endometriotic Cells and the Alternative Activation of Endometriosis-Associated Macrophages." Am J Chin Med. [Epub ahead of print] Application: Human cell culture supernatants. Kalinowska-Lyszczarz A et al. (2018) "Immune-cell BDNF expression in treatment-naive relapsing-remitting multiple sclerosis patients and following one year of immunomodulation therapy." Neurol Neurochir Pol. [In Press] Application: Human PBMC cell lysates. Lindsay SL et al. (2016) "Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells." Stem Cell Reports. 6(5):729-42 Application: MSC-conditioned medium/cell supernatant. Carnevale G et al. (2016) "Human dental pulp stem cells expressing STRO-1, c-kit and CD34 markers in peripheral nerve regeneration." J Tissue Eng Regen Med. [Epub ahead of print] Application: Supernatant of human dental pulp stem cells (hDPSCs).
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the mouse and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The Biosensis proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Serum, Plasma (Heparin), Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021). ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020). Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Rowe CW et al. (2019). The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis. BMC Endocr Disord. 19(1):128. Application: Human serum. Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: Human primary culture supernatant. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Human urine. Sherif IO & Al-Gayyar MMH (2018). Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Specificity:
Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
The Biosensis proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Serum, Plasma (Heparin), Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021). ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020). Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Rowe CW et al. (2019). The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis. BMC Endocr Disord. 19(1):128. Application: Human serum. Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: Human primary culture supernatant. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Human urine. Sherif IO & Al-Gayyar MMH (2018). Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Specificity:
Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
The Biosensis Mouse Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2213) and full-length proNGF (BEK-2236). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants and tissue homogenates only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Researchers have used both kits for NGF/proNGF measurement in mouse urine, however, Biosensis has not yet internally validated the use of urine in both ELISA kits. End-users are strongly advised to perform essential validation experiments to assure accurate NGF/proNGF quantification in mouse urine. Please refer to the most current kit protocols for BEK-2213 (Mature BDNF Rapid TM ELISA) and BEK-2236 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a native mouse NGF protein as standard, purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. The proNGF ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli. Note that this Mature NGF/proNGF Combo kit is designed to measure the mouse protein forms, although due to sequence homology the Mature NGF ELISA kit will detect human and rat mature NGF. The proNGF ELISA kit cross-reacts with rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. However, the proNGF ELISA shows only little cross-reactivity (20%) with human proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
See BEK-2213 & BEK-2236 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2213 (Mouse Mature NGF Rapid TM ELISA Kit) and BEK-2236 (Mouse/Rat proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects mouse NGF, and cross-reacts with human and rat mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects mouse and rat proNGF only, and shows only little reactivity (20%) with human proNGF. The proNGF ELISA detects the full-length form of proNGF only, and does not quantify mature NGF or the pro-domain peptide.
Typical limit of detection (LOD) for mouse mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.<br><br><b>proNGF ELISA:</b><br>Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
The Biosensis Human Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2212) and full-length proNGF (BEK-2226). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants, serum, and plasma (citrate) only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Researchers have used both kits for NGF/proNGF measurement in human urine, however, Biosensis has not yet internally validated the use of urine in both ELISA kits. End-users are strongly advised to perform essential validation experiments to assure accurate NGF/proNGF quantification in human urine. Please refer to the most current kit protocols for BEK-2212 (Mature BDNF Rapid TM ELISA) and BEK-2226 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a high-quality recombinant human NGF standard approved by the World Health Organization (WHO). The proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. Note that this Mature NGF/proNGF Combo kit is designed to measure the human protein forms, although due to sequence homology the Mature NGF ELISA kit will detect mouse and rat mature NGF. The proNGF ELISA kit does not cross-react with rodent forms of proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates Mossa AH et al. (2020) . The antibodies used in the proNGF ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Important: Accurate NGF quantification in human citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-005-500 . Accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 . Both Heterophilic Antibody Blockers are provided in the kit. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
See BEK-2212 & BEK-2226 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Serum, Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2212 (Human Mature NGF Rapid TM ELISA Kit) and BEK-2226 (Human proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects human NGF, and cross-reacts with mouse and rat mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects human proNGF only, does not cross-react with mouse and rat proNGF. Both capture and detection antibodies used in the proNGF ELISA kit binds to epitopes within the pro-domain of proNGF. Thus, the proNGF ELISA detects the full-length form of proNGF, and does not quantify mature NGF.
Typical limit of detection (LOD) for human mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for human proNGF is < 60 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in the Mature NGF ELISA kit bind epitopes within the mature domain of the protein. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), demonstrating the preferential quantification of mature NGF over full-length human proNGF. The absence of proNGF cross-reactivity in the mature NGF ELISA kit has been independently confirmed by <a class="newA" target="_blank" href="https://pubmed.ncbi.nlm.nih.gov/32870355/">Mossa AH <i>et al.</I> (2020)</a>. The Mature NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat!<br><br><b>proNGF ELISA:</b><br>Does not cross-react with recombinant human mature NGF and proBDNF tested at 25 ng/mL. Does not react with rodent proNGF.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF. Guinea pig GDNF has been quantified in serum using the Human GDNF Rapid TM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Human recombinant GDNF with N-terminal methionine residue.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Widbiller M et al. (2019) Neurotrophic Proteins in Dentin and Their Effect on Trigeminal Sensory Neurons. J Endod. [Epub ahead of print]. Application: Human tooth homogenate.
Specificity:
Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF. Guinea pig GDNF has been quantified in serum using the Human GDNF Rapid TM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Human recombinant GDNF with N-terminal methionine residue.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Widbiller M et al. (2019) Neurotrophic Proteins in Dentin and Their Effect on Trigeminal Sensory Neurons. J Endod. [Epub ahead of print]. Application: Human tooth homogenate.
Specificity:
Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis NT3 Rapid TM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-004-500 ). This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 Rapid TM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid TM ELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant NT3, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-3 (NT-3) in Culture Supernatant, Plasma (Citrate), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Bavaresco DV et al. (2018) Depressive symptoms and neurotrophin levels in ostomy patients. J Bras Psiquiatr. 67(3). Application: Human serum. Shen W et al. (2017) Effects of Ranibizumab and Aflibercept on Human Mueller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 ELISA will also react with those species.
The Biosensis NT3 Rapid TM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-004-500 ). This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 Rapid TM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid TM ELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant NT3, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-3 (NT-3) in Culture Supernatant, Plasma (Citrate), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Bavaresco DV et al. (2018) Depressive symptoms and neurotrophin levels in ostomy patients. J Bras Psiquiatr. 67(3). Application: Human serum. Shen W et al. (2017) Effects of Ranibizumab and Aflibercept on Human Mueller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid ELISA will also react with those species.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Culture Supernatant, Urine, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse plasma. Zabbarova IV et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn. 2018 May 28 [Epub ahead of print]. Application: Mouse urine. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Specificity:
Mouse. The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Storage:
Store at 2-8°C.
Range:
62.5 - 4,000 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates,Urine
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Culture Supernatant, Urine, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse plasma. Zabbarova IV et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn. 2018 May 28 [Epub ahead of print]. Application: Mouse urine. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Specificity:
Mouse. The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Storage:
Store at 2-8°C.
Range:
62.5 - 4,000 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates,Urine
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
The Biosensis Neurotrophin 4/5 Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-003-1000 ). This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards. This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey. The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4/5, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-4/5 (NT-4/5) in Culture Supernatant, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Ishimoto T et al. (2018) Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4/5-TrkB signaling in murine neural stem cells. Cell Signal. [Epub ahead of print]. Application: Mouse cell lysate and brain homogenate. Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates. Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.
Specificity:
Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
The Biosensis Neurotrophin 4/5 Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-003-1000 ). This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards. This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey. The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4/5, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-4/5 (NT-4/5) in Culture Supernatant, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Ishimoto T et al. (2018) Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4/5-TrkB signaling in murine neural stem cells. Cell Signal. [Epub ahead of print]. Application: Mouse cell lysate and brain homogenate. Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates. Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.
Specificity:
Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Alpha-Synuclein Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of alpha-synuclein in less than 4 hours in human citrate-plasma, serum, CSF, as well as mouse and rat brain homogenates only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application. This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit has not been tested for other substrate applications, only citrate plasma and CSF, other substrates have not been tested and performance may vary. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The alpha-synuclein ELISA kit employs a recombinant human standard expressed in E.coli.
Applications:
ELISA
Application Details:
ELISA. For the quantification of alpha-synuclein in human serum, plasma (citrate), CSF, and mouse/rat tissue homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human, mouse and rat alpha-synuclein.
Storage:
Store at 2-8°C
Range:
0.16 - 10 ng/mL
Sample Type:
CSF,Plasma (Citrate),Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Interference and cross-reactivity of human beta- and gamma-synuclein was assessed by spiking each protein at excess concentration of 20 ng/mL into two human plasma samples, each diluted 1:10 and 1:20, in comparison to unspiked samples. Cross-reactivity was calculated based on increase or decrease of apparent ?-synuclein concentrations in spiked samples. At a 3-6 fold excess (w/v) of spiked protein over endogenous alpha-synuclein at both sample dilutions, this ELISA shows very little cross-reactivity of 2.3% or less.<br>Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.
The Biosensis Alpha-Synuclein Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of alpha-synuclein in less than 4 hours in human citrate-plasma, serum, CSF, as well as mouse and rat brain homogenates only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application. This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit has not been tested for other substrate applications, only citrate plasma and CSF, other substrates have not been tested and performance may vary. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The alpha-synuclein ELISA kit employs a recombinant human standard expressed in E.coli.
Applications:
ELISA
Application Details:
ELISA. For the quantification of alpha-synuclein in human serum, plasma (citrate), CSF, and mouse/rat tissue homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human, mouse and rat alpha-synuclein.
Storage:
Store at 2-8°C
Range:
0.16 - 10 ng/mL
Sample Type:
CSF,Plasma (Citrate),Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Interference and cross-reactivity of human beta- and gamma-synuclein was assessed by spiking each protein at excess concentration of 20 ng/mL into two human plasma samples, each diluted 1:10 and 1:20, in comparison to unspiked samples. Cross-reactivity was calculated based on increase or decrease of apparent ?-synuclein concentrations in spiked samples. At a 3-6 fold excess (w/v) of spiked protein over endogenous alpha-synuclein at both sample dilutions, this ELISA shows very little cross-reactivity of 2.3% or less.<br>Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.
The oligomeric form of Amyloid Beta peptide (A?, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting A? have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al. , 2012) , has been shown to specifically detect A?, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of A? peptide (o-A?) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. The Biosensis oligomeric A? ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric A? peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-A? capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-A? present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric A? peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-A? standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human,Rat
Immunogen:
The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kasus-Jacobi A et al. (2022) "Selecting Multitarget Peptides for Alzheimers Disease" Biomolecules. 12, 1386 Application: Human, A?142 oligomers. Eid A et al. (2022) "Effects of DDT on Amyloid Precursor Protein Levels and Amyloid Beta Pathology: Mechanistic Links to Alzheimer's Disease Risk" Environ Health Perspect. [Epub ahead of print] Application: Mouse, brain tissue homogenate. Kasus-Jacobi A et al. (2021) "Neutrophil Granule Proteins Inhibit Amyloid Beta Aggregation and Neurotoxicity." Curr Alzheimer Res. 18(5):414-427 Application: Mouse in-vitro assay, cell culture supernatant. Hark TJ et al. (2020) "Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer s Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals." Cell Syst. [Epub ahead of print] Application: Mouse cortical homogenates. Xiao L et al. (2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells." Neural Regen Res. 15(12): 2270-2 Application: Rat PC12 RIPA cell extract. Hrynchak MV et al. (2020) "Chronic Presence of Oligomeric A? Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression." Front Synaptic Neurosci. Apr 24;12:16 Application: Mouse lysate. El-Sayed NA et al. (2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents." Bioorg Chem. [Epub ahead of print] Application: Human. In-vitro screening of drug candidates. Oh Joo Kweon, Young Chul Youn, Yong Kwan Lim, Mi-Kyung Lee, Hye Ryoun Kim (2019) "Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease." J Neurol Sci. [In press] Application: Human serum. Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal A? aggregation and limits secretion of A? via exosomes." FASEB J. [Epub ahead of print] Application: Human cell line, mouse brain and organotypic brain slice cultures. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis." Brain Pathol. [Epub ahead of print] Application: Transgenic mouse brain homogenates. S Liu, S Park, G Allington, F Prelli, Y Sun, M Marta-Ariza, H Scholtzova, G Biswas, B Brown, PB Verghese, PD Mehta, Y-U Kwon and T Wisniewski (2017) "Targeting Apolipoprotein E/Amyloid _ Binding by Peptoid CPO_A?17-21 P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline." Sci Rep. 7(1):8009 Application: Transgenic mouse brain homogenates. M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models." Transl Psychiatry. Jan 31;7(1):e1022. Application: Extracts of E3FAD and E4FAD transgenic mouse brains. Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice." Acta Neuropathol Commun. 4(1):111 Application: Tris-extracts of EFAD transgenic mouse brains. Nor Faeizah Ibrahim, Daijiro Yanagisawa, Lina Wati Durani, Hamizah Shahirah Hamezah, Hanafi Ahmad Damanhuri, Wan Zurinah Wan Ngah, Mayumi Tsuji, Yuji Kiuchi, Kenjiro Ono, Ikuo Tooyama (2016) "Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in A?PP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]. Application: Tris-extracts of mouse brain homogenates. Jia Luo, Sue H. Lee, Lawren VandeVrede, Zhihui Qin, Manel Ben Aissa, John Larson, Andrew F. Teich, Ottavio Arancio, Yohan D'Souza, Ahmed Elharram, Kevin Koster, Leon M. Tai, Mary Jo LaDu, Brian M. Bennett and Gregory R. J. Thatcher (2016) "A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease." Molecular Neurodegeneration 2016 11:35. Application: Tris-extracts of EFAD transgenic mouse brains. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." Neurobiology of Disease. Vol. 93, Pages 215-225 Application: TBSX-extracts of mouse cerebral cortex. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice." Neurobiology of Aging. Volume 37, January 2016, Pages 47-57 Application: Tris-extracts of E3FAD and E4FAD transgenic mouse brains. Combes M, Poindron P, Callizot N.(2015) "Glutamate protects neuromuscular junctions from deleterious effects of ?-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture." J Neurosci. Res. 93(4):633-43 Application: Native Rat neurites & human muscle cell co-culture supernatants. Seo, Dong Han, et al. (2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors." Green Chem. 17:2164-2171. Application: Synthetic constructs. Tai, LM (2014) "Amyloid-_ Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo." J Biol Chem. 289(44):30538-55 Application: EFAD-Tg mice. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology." (2014) J Neurosci. Sep 24;34(39):12982-99 Application: Transgenic Mouse brain lysates, supernatants.
Specificity:
Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 and is highly specific just to amyloid beta peptide. The Biosensis o-A? Elisa detects A? oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015). Rat.
The oligomeric form of Amyloid Beta peptide (A?, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting A? have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al. , 2012) , has been shown to specifically detect A?, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of A? peptide (o-A?) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. The Biosensis oligomeric A? ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric A? peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-A? capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-A? present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric A? peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-A? standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human,Rat
Immunogen:
The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kasus-Jacobi A et al. (2022) "Selecting Multitarget Peptides for Alzheimers Disease" Biomolecules. 12, 1386 Application: Human, A?142 oligomers. Eid A et al. (2022) "Effects of DDT on Amyloid Precursor Protein Levels and Amyloid Beta Pathology: Mechanistic Links to Alzheimer's Disease Risk" Environ Health Perspect. [Epub ahead of print] Application: Mouse, brain tissue homogenate. Kasus-Jacobi A et al. (2021) "Neutrophil Granule Proteins Inhibit Amyloid Beta Aggregation and Neurotoxicity." Curr Alzheimer Res. 18(5):414-427 Application: Mouse in-vitro assay, cell culture supernatant. Hark TJ et al. (2020) "Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer s Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals." Cell Syst. [Epub ahead of print] Application: Mouse cortical homogenates. Xiao L et al. (2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells." Neural Regen Res. 15(12): 2270-2 Application: Rat PC12 RIPA cell extract. Hrynchak MV et al. (2020) "Chronic Presence of Oligomeric A? Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression." Front Synaptic Neurosci. Apr 24;12:16 Application: Mouse lysate. El-Sayed NA et al. (2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents." Bioorg Chem. [Epub ahead of print] Application: Human. In-vitro screening of drug candidates. Oh Joo Kweon, Young Chul Youn, Yong Kwan Lim, Mi-Kyung Lee, Hye Ryoun Kim (2019) "Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease." J Neurol Sci. [In press] Application: Human serum. Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal A? aggregation and limits secretion of A? via exosomes." FASEB J. [Epub ahead of print] Application: Human cell line, mouse brain and organotypic brain slice cultures. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis." Brain Pathol. [Epub ahead of print] Application: Transgenic mouse brain homogenates. S Liu, S Park, G Allington, F Prelli, Y Sun, M Marta-Ariza, H Scholtzova, G Biswas, B Brown, PB Verghese, PD Mehta, Y-U Kwon and T Wisniewski (2017) "Targeting Apolipoprotein E/Amyloid _ Binding by Peptoid CPO_A?17-21 P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline." Sci Rep. 7(1):8009 Application: Transgenic mouse brain homogenates. M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models." Transl Psychiatry. Jan 31;7(1):e1022. Application: Extracts of E3FAD and E4FAD transgenic mouse brains. Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice." Acta Neuropathol Commun. 4(1):111 Application: Tris-extracts of EFAD transgenic mouse brains. Nor Faeizah Ibrahim, Daijiro Yanagisawa, Lina Wati Durani, Hamizah Shahirah Hamezah, Hanafi Ahmad Damanhuri, Wan Zurinah Wan Ngah, Mayumi Tsuji, Yuji Kiuchi, Kenjiro Ono, Ikuo Tooyama (2016) "Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in A?PP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]. Application: Tris-extracts of mouse brain homogenates. Jia Luo, Sue H. Lee, Lawren VandeVrede, Zhihui Qin, Manel Ben Aissa, John Larson, Andrew F. Teich, Ottavio Arancio, Yohan D'Souza, Ahmed Elharram, Kevin Koster, Leon M. Tai, Mary Jo LaDu, Brian M. Bennett and Gregory R. J. Thatcher (2016) "A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease." Molecular Neurodegeneration 2016 11:35. Application: Tris-extracts of EFAD transgenic mouse brains. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." Neurobiology of Disease. Vol. 93, Pages 215-225 Application: TBSX-extracts of mouse cerebral cortex. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice." Neurobiology of Aging. Volume 37, January 2016, Pages 47-57 Application: Tris-extracts of E3FAD and E4FAD transgenic mouse brains. Combes M, Poindron P, Callizot N.(2015) "Glutamate protects neuromuscular junctions from deleterious effects of ?-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture." J Neurosci. Res. 93(4):633-43 Application: Native Rat neurites & human muscle cell co-culture supernatants. Seo, Dong Han, et al. (2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors." Green Chem. 17:2164-2171. Application: Synthetic constructs. Tai, LM (2014) "Amyloid-_ Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo." J Biol Chem. 289(44):30538-55 Application: EFAD-Tg mice. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology." (2014) J Neurosci. Sep 24;34(39):12982-99 Application: Transgenic Mouse brain lysates, supernatants.
Specificity:
Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 and is highly specific just to amyloid beta peptide.The Biosensis o-A? Elisa detects A? oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015). Rat.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit (BEK-2213). Cross-reaction of full-length proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Guinea Pig,Rat
Immunogen:
Sf 21 (baculovirus)-derived rat beta-NGF protein
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Mossa A et al. (2021) "Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder." Exp Gerontol. [Epup ahead of print] Application: Rat urine. Itai S et al. (2020) "Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration." Biomed Microdevices. 22(4):81 Application: Rat cell culture supernatant. Cheppudira BP et al. (2016) "Anti-nerve growth factor antibody attenuates chronic morphine treatment-induced tolerance in the rat." BMC Anesthesiol. 16:73 Application: Acid-extracted rat spinal cord homogenate.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for rat NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! <br><br>Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings. Data was obtained with the Mouse NGF ELISA kit (BEK-2213) which uses the same assay capture and detection antibodies
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit (BEK-2213). Cross-reaction of full-length proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Guinea Pig,Rat
Immunogen:
Sf 21 (baculovirus)-derived rat beta-NGF protein
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Mossa A et al. (2021) "Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder." Exp Gerontol. [Epup ahead of print] Application: Rat urine. Itai S et al. (2020) "Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration." Biomed Microdevices. 22(4):81 Application: Rat cell culture supernatant. Cheppudira BP et al. (2016) "Anti-nerve growth factor antibody attenuates chronic morphine treatment-induced tolerance in the rat." BMC Anesthesiol. 16:73 Application: Acid-extracted rat spinal cord homogenate.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for rat NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! <br><br>Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings. Data was obtained with the Mouse NGF ELISA kit (BEK-2213) which uses the same assay capture and detection antibodies.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A mouse NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Native mouse NGF, purified from submaxillary glands
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Xu J et al. (2022) NGF-p75 signaling coordinates skeletal cell migration during bone repair Sci Adv. 8(11):eabl5716. Application: Mouse cell lysates. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse hippocampal RIPA homogenates. Sugimoto J et al. (2021) Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). La Porta C and Tappe-Theodor A (2020) Differential impact of psychological and psychophysical stress on low back pain in mice. Pain. 161(7):1442-1458. Application: Mouse tissue homogenate. Mossa AH et al. (2020) Antagonism of proNGF or its receptor p75 NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Roy S et al. (2020) Neurogenic Tissue Nanotransfection in the Management of Cutaneous Diabetic Polyneuropathy. Nanomedicine. [Epub ahead of print]. Application: Mouse lysate. Vigli D et al. (2018) Chronic treatment with the phytocannabinoid Cannabidivarin (CBDV) rescues behavioural alterations and brain atrophy in a mouse model of Rett syndrome. Neuropharmacology. 2018 Jul 26; [Epub ahead of print]. Application: Mouse hippocampus homogenate. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for mouse NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! <br><br>Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A mouse NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Native mouse NGF, purified from submaxillary glands
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Xu J et al. (2022) NGF-p75 signaling coordinates skeletal cell migration during bone repair Sci Adv. 8(11):eabl5716. Application: Mouse cell lysates. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse hippocampal RIPA homogenates. Sugimoto J et al. (2021) Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). La Porta C and Tappe-Theodor A (2020) Differential impact of psychological and psychophysical stress on low back pain in mice. Pain. 161(7):1442-1458. Application: Mouse tissue homogenate. Mossa AH et al. (2020) Antagonism of proNGF or its receptor p75 NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Roy S et al. (2020) Neurogenic Tissue Nanotransfection in the Management of Cutaneous Diabetic Polyneuropathy. Nanomedicine. [Epub ahead of print]. Application: Mouse lysate. Vigli D et al. (2018) Chronic treatment with the phytocannabinoid Cannabidivarin (CBDV) rescues behavioural alterations and brain atrophy in a mouse model of Rett syndrome. Neuropharmacology. 2018 Jul 26; [Epub ahead of print]. Application: Mouse hippocampus homogenate. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for mouse NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! <br><br>Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum and plasma samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate 3,3',5,5'-tetramethylbenzidine, TMB yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A human NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure human NGF and thus employs a recombinant human NGF standard approved by the World Health Organization (WHO). Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from numerous other species including mouse and rat! The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, internal validation data demonstrates Note that accurate NGF quantification in human citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-005-500 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human NGF, made in CHO cells (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. Chen S et al. (2021) Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation. J Med Investig. Vol. 68. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020) Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human Plasma. Sherif IO & Al-Gayyar MMH (2018) Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Typical limit of detection (LOD) for human NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), suggesting the preferential quantification of mature NGF over full-length human proNGF.<br><br> This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat!
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum and plasma samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate 3,3',5,5'-tetramethylbenzidine, TMB yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A human NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure human NGF and thus employs a recombinant human NGF standard approved by the World Health Organization (WHO). Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from numerous other species including mouse and rat! The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, internal validation data demonstrates Note that accurate NGF quantification in human citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-005-500 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human NGF, made in CHO cells (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. Chen S et al. (2021) Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation. J Med Investig. Vol. 68. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020) Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human Plasma. Sherif IO & Al-Gayyar MMH (2018) Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Typical limit of detection (LOD) for human NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), suggesting the preferential quantification of mature NGF over full-length human proNGF.<br><br> This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat!
The Biosensis CE Marked BDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. This BDNF ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognize the mature as well as the pro-form of BDNF. However, cross-reactivity to the full-length proBDNF protein is low. This CE Marked BDNF Rapid ELISA [Cat. No. BEK-2211-CE] Kit is approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. ( www.calbiotech.com ) for Biosensis. BEK-2211-CE is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211 . This research-use-only ELISA kit can be used for human and animal research purposes worldwide, and has been validated for a wider range of sample types and species.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Serum, Plasma (Citrate). BEK-2211-CE is expressly designed and tested only for use on human blood and plasma samples. Any other use is deemed "off label use" and thus the performance characteristics of the assay will have to be determined by the end user, and such results are not supported by Biosensis or CalBioTech at this time. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
See BEK-2211-2P-CE protocol insert for specific expiration dating of the kit and its components.
Use:
Approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. (www.calbiotech.com) for Biosensis.
This kit is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Reed JL et al. (2021) The effects of high-intensity interval training, Nordic walking and moderate-to-vigorous intensity continuous training on functional capacity, depression and quality of life in patients with coronary artery disease enrolled in cardiac rehabilitation: A randomized controlled trial (CRX study). Prog Cardiovasc Dis. [Epub ahead of print]. Application: Human blood. Valkenborghs SR et al. (2019) Aerobic exercise and consecutive task-specific training (AExaCTT) for upper limb recovery after stroke: A randomized controlled pilot study. Physiother Res Int. [Epub ahead of print]. Application: Human serum.
Specificity:
Human BDNF when used as directed.
Storage:
Store at 2-8°C
Range:
7.8 pg/mL - 500 pg/mL
Sample Type:
Plasma (Citrate),Serum
Sensitivity:
Typical limit of detection (LOD) for BDNF is < 3 pg/mL determined by calculating the mean + 2x standard deviation of mean of blank (n=20).
The Biosensis Mature BDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), pig serum, cell lysates, brain extracts, human milk and Sheep CSF only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples, human milk and CSF. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately ( BL-001-1250 ). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com . This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015). This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance. This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. Extensive validation has demonstrated that the Mature BDNF Rapid TM ELISA shows only minimal cross-reactivity with proBDNF. Please refer to our Technical Note #5 for further details on ELISA assay validation for BDNF isoform detection and quanification. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF ELISA kit (BEK-2211-CE) .
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates, Human Milk, CSF. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Total Number of References: 99 Latest Publications (2019-2022):
Meshkat S et al. (2022) Brain-Derived Neurotrophic Factor (BDNF) as a biomarker of treatment response in patients with Treatment Resistant Depression (TRD): A systematic review & meta-analysis Psychiatry Res. 317:114857 Application: Human, serum. Cook A et al. (2022) Activation of TrkB-Akt signaling rescues deficits in a mouse model of SCA6 Sci Adv. [Epub ahead of print] Application: Mouse, brain extracts. Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Salem HA et al. (2022) Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis Pharmaceuticals. 15, 1012. Application: Rat, brain supernatant. Aldhshan MS & Mizuno TM. (2022) Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice Behav Brain Res. [Epub ahead of print]. Application: Mouse, brain tissue homogenate. Fujino M et al. (2022) Orally Administered Plasmalogens Alleviate Negative Mood States and Enhance Mental Concentration: A Randomized, Double-Blind, Placebo-Controlled Trial Front Cell Dev Biol. 10:894734 Application: Human, plasma. Abrial E et al. (2022) Investigating Predictive Factors of Suicidal Re-attempts in Adolescents and Young Adults After a First Suicide Attempt, a Prospective Cohort Study. Study Protocol of the SURAYA Project Front. Psychiatry. [Epub ahead of print] Application: Human, plasma. Tanaka-Kanegae R et al. (2022) Sufficiently Elevated Core Body Temperature May Be Necessary to Maintain Cerebral Blood Flow Response throughout the Morning Neurosci Med. 13, 70-90 Application: Human, serum. Merlo S et al. (2022) Microglial polarization differentially affects neuronal vulnerability to the ?-amyloid protein: Modulation by melatonin Biochem Pharmacol. 202:115151 Application: Human, cell culture supernatant. Dalile B et al. (2022) Extruded Wheat Bran Consumption Increases Serum Short-Chain Fatty Acids but Does Not Modulate Psychobiological Functions in Healthy Men: A Randomized, Placebo-Controlled Trial Front Nutr. 9:896154 Application: Human, serum. Agapouda A et al. (2022) Rhodiola Rosea Extract Counteracts Stress in an Adaptogenic Response Curve Manner via Elimination of ROS and Induction of Neurite Outgrowth Oxid. Med. Cell. Longev. [Epub ahead of print] Application: Human, cell lysates. Breazeale S et al. (2022) Symptom cluster profiles following traumatic orthopaedic injuries Injury. [Epub ahead of print] Application: Human, serum. Jaehne EJ et al. (2022) Behavioral phenotyping of a rat model of the BDNF Val66Met polymorphism reveals selective impairment of fear memory Transl Psychiatry. 12(1):93 Application: Rat, acid extracted tissue lysates. Wang RY et al. (2022) The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats. Int J Mol Sci. 23, 1780 Application: Rat, brain tissue homogenate and serum. Hugues N et al. (2022) Time-Dependent Cortical Plasticity during Moderate-Intensity Continuous Training Versus High-Intensity Interval Training in Rats. Cereb Cortex. [Epub ahead of print] Application: Rat, cortical tissue homogenate. Cefis M et al. (2021) Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep. 12(1):311 Application: Rat, skeletal muscle tissue homogenate. Becker AM et al. (2021) Acute Effects of Psilocybin After Escitalopram or Placebo Pretreatment in a Randomized, Double-Blind, Placebo-Controlled, Crossover Study in Healthy Subjects. Clin Pharmacol Ther. [Epub ahead of print] Application: Human, plasma. Berbenetz N et al. (2021) The Relationship Between Brain Derived Neurotrophic Factor (BDNF) and Symptoms Following Catheter Ablation for Paroxysmal Atrial Fibrillation (AF)- NEURO-AF Study. Circulation. [Epub ahead of print] Application: Human, serum. Walsh JJ et al. (2021) Short-term ketone monoester supplementation improves cerebral blood flow and cognition in obesity: A randomized cross-over trial. J Physiol. [Epub ahead of print] Application: Human, serum, platelet-poor plasma. Boukhatem I et al. (2021) The brain-derived neurotrophic factor prompts platelet aggregation and secretion. Blood Adv. 5(18):3568-3580 Application: Human, plasma. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765 Application: Human, serum. Inoue T et al. (2021) Ipsilateral BDNF mRNA expression in the motor cortex positively correlates with motor function of the affected forelimb after intracerebral hemorrhage. Brain Res. [Epub ahead of print] Application: Rat, brain homogenate. Shoshina II et al. (2021) Visual processing and BDNF levels in first-episode schizophrenia. Psychiatry Res. [Epub ahead of print] Application: Human, serum. Cappoli N et al. (2021) Effects of remifentanil on human C20 microglial pro-inflammatory activation. Eur Rev Med Pharmacol Sci. 25(16):5268-5274 Application: Human, cell culture. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print] Application: Human, urine. Mori Y et al. (2021) Serum BDNF as a Potential Biomarker of Alzheimer's Disease: Verification Through Assessment of Serum, Cerebrospinal Fluid, and Medial Temporal Lobe Atrophy. Front Neurol. 12:653267 Application: Human, serum. Seno S et al. (2021) Effects of Selective Serotonin Reuptake Inhibitors on Depression-Like Behavior in a Laser-Induced Shock Wave Model. Front Neurol. 12:602038 Application: Mouse, hippocampal homogenates. Medeiros GC et al. (2021) Treatment of depression with ketamine does not change plasma levels of brain-derived neurotrophic factor or vascular endothelial growth factor. J Affect Disord. 280(Pt A):136-139 Application: Human, plasma. Dorandish S et al. (2021) Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media. Int J Mol Sci. 22(13):7059 Application: Human, culture supernatant. Yap NY et al. (2021) Relationship between cytokines and brain-derived neurotrophic factor (BDNF) in trajectories of cancer-related cognitive impairment. Cytokine. [Epub ahead of print] Application: Human, plasma. Wang L et al. (2021) The mediating effect of brain-derived neurotrophic factor levels on childhood trauma and psychiatric symptoms in patients with first-episode schizophrenia. Aust N Z J Psychiatry. [Epub ahead of print] Application: Human. Mallik SB et al. (2021) Remedial effects of caffeine against depressive-like behaviour in mice by modulation of neuroinflammation and BDNF. Nutr Neurosci. [Epub ahead of print] Application: Mouse. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179 Application: Mouse, hippocampal RIPA homogenates. Caruso GI et al. (2021) SIRT1-Dependent Upregulation of BDNF in Human Microglia Challenged with A?: An Early but Transient Response Rescued by Melatonin. Biomedicines. 9(5):466 Application: Human, cell culture supernatant. Miller KM et al. (2021) Striatal Afferent BDNF Is Disrupted by Synucleinopathy and Partially Restored by STN DBS. J Neurosci. 41(9):2039-52 Application: Rat, tissue homogenates (RIPA). Vickneson K et al. (2021) Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia. Diabetologia. 64(6):1436-41 Application: Rat, blood. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Pan S et al. (2021) The microRNA-195 - BDNF pathway and cognitive deficits in schizophrenia patients with minimal antipsychotic medication exposure. Transl Psychiatry. 11(1):117 Application: Human, plasma. Miyamoto T et al. (2021) Effect of pedaling cadence on serum levels of brain-derived neurotrophic factor during ergometric exercise in healthy adults. Sport Sci Health. Application: Human, serum. Normann AJ (2020) The Effect of Light Therapy and Acute Aerobic Exercise on Serum Brain Derived Neurotrophic Factor in Older Adults. MSc Thesis. Application: Human, serum. Holze F et al. (2020) Acute dose-dependent effects of lysergic acid diethylamide in a double-blind placebo-controlled study in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human, plasma. Hasler G et al. (2020) The Association Between Adolescent Residential Mobility and Adult Social Anxiety, BDNF and Amygdala-Orbitofrontal Functional Connectivity in Young Adults With Higher Education. Front. Psychiatry. Application: Human, serum. Wallace AW (2020) The Impact of Six Weeks of Intermittent Fasting, With and Without Aerobic Exercise, on Serum BDNF in Young Adult Males. MSc Thesis. Application: Human, serum. Walsh JJ et al. (2020) The Effect of Exogenous Ketone Monoester Ingestion on Plasma BDNF During an Oral Glucose Tolerance Test. Front Physiol. 11:1094. Application: Human, plasma. Meade GM et al. (2020) A Model of Negative Emotional Contagion Between Male-Female Rat Dyads: Effects of Voluntary Exercise on Stress-Induced Behavior and BDNF-TrkB Signaling. Physiol Behav. 113286 Application: Rat, serum. Hutten NRPW et al. (2020) Low Doses of LSD Acutely Increase BDNF Blood Plasma Levels in Healthy Volunteers. ACS Pharmacol. Transl. Sci. Application: Human, plasma. Barbosa AC et al. (2020) Assessment of BDNF serum levels as a diagnostic marker in children with autism spectrum disorder. Sci Rep. 10(1):17348. Application: Human, serum. Okamura M et al. (2020) Low-Level Inhibition of GABAergic Synapses Enhances Gene Expressions Crucial for Neuronal Plasticity in the Hippocampus After Ischemic Stroke. J Stroke Cerebrovasc Dis. 29(12):105316. Application: Rat, hippocampus homogenate. Payne AJ et al. (2020) The Effects of Alcohol on BDNF and CD5 Dependent Pathways. PhD Thesis. Application: Mouse, RIPA tissue homogenate. Nishiyama M et al. (2020) Homostachydrine is a Xenobiotic Substrate of OCTN1/SLC22A4 and Potentially Sensitizes Pentylenetetrazole-Induced Seizures in Mice. Neurochem Res. [Epub ahead of print]. Application: Mouse, acid-extracted hippocampal homogenate. Lorinczova HT et al. (2020) Co-Administration of Iron and a Bioavailable Curcumin Supplement Increases Serum BDNF Levels in Healthy Adults. Antioxidants (Basel). 9(8):E645. Application: Human serum. Yap NY F et al. (2020) Associations of plasma brain-derived neurotrophic factor (BDNF) and Val66Met polymorphism (rs6265) with long-term cancer-related cognitive impairment in survivors of breast cancer. Breast Cancer Res Treat. [Epub ahead of print]. Application: Human plasma. Vasilopoulou F et al. (2020) Amelioration of BPSD-Like Phenotype and Cognitive Decline in SAMP8 Mice Model Accompanied by Molecular Changes After Treatment With I 2-Imidazoline Receptor Ligand MCR5. Pharmaceutics. 12(5):E475. Application: Mouse hippocampus RIPA-homogenates. Mueller ST et al. (2020) Negative Association Between Left Prefrontal GABA Concentration and BDNF Serum Concentration in Young Adults. Heliyon. 6(5):e04025. Application: Human serum. Chen LF et al. (2020) The NMDA receptor subunit GluN3A regulates synaptic activity-induced and myocyte enhancer factor 2C (MEF2C)-dependent transcription. J Biol Chem. [Epub ahead of print]. Application: Rat neuronal cell lysate, acid-extracted. Companys-Alemany J et al. (2020) A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice. Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Furukawa Y et al. (2020) Citrus Auraptene Induces Expression of Brain-Derived Neurotrophic Factor in Neuro2a Cells. Molecules. 25(5). Application: Mouse Neuro2a culture supernatant. Holze F et al. (2019) Distinct acute effects of LSD, MDMA, and D-amphetamine in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human plasma. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human plasma. Sartori A et al. (2019) Interferon-beta, but not Glatiramer Acetate treatment induces gender-specific increase in BDNF serum levels in relapsing-remitting multiple sclerosis female patients. Res J Neuro N Disord. 1:5-18. Application: Human serum. Please refer to our Technical Note #5 for validation experiments disproving the author's claim that the Biosensis Mature BDNF Rapid TM ELISA quantifies total BDNF! Vanicek T et al. (2019) Repetitive Enhancement of Serum BDNF Subsequent to Continuation ECT. Acta Psychiatr Scand. [Epub ahead of print]. Application: Human serum. Gejl AK et al. (2019) Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy. Sci Rep. 9(1):9655. Application: Human serum and EDTA-plasma. Li X et al. (2019) Exercise enhances the expression of brain-derived neurotrophic factor in the hippocampus accompanied by epigenetic alterations in senescence-accelerated mice prone 8. Neurosci Lett. [Epub ahead of print]. Application: Mouse brain homogenates. Yang CY et al. (2019) Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats. Neurotox Res. [Epub ahead of print]. Application: Rat brain homogenates. Du Y et al. (2019) Genome-Wide, Integrative Analysis Implicates Exosome-Derived MicroRNA Dysregulation in Schizophrenia. Schizophr Bull. [Epub ahead of print]. Application: Human serum. Vanicek T et al. (2019) Acute and Subsequent Continuation Electroconvulsive Therapy Elevates Serum BDNF Levels in Patients with Major Depression. Brain Stimul. [In press]. Application: Human serum, plasma. Duart-Castells L et al. (2019) 7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway. Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates.
Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value.
Cross Reactivity:
No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM). Additional proBDNF cross-reactivity experiments were conducted as summarized in our <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/Technical-Note-5-Mature-BDNF-Isoform-Detection-and-Quantification-by-ELISA.pdf">Technical Note #5</a>.
The Biosensis CE Marked BDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. This BDNF ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognize the mature as well as the pro-form of BDNF. However, cross-reactivity to the full-length proBDNF protein is low. This CE Marked BDNF Rapid ELISA [Cat. No. BEK-2211-CE] Kit is approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. ( www.calbiotech.com ) for Biosensis. BEK-2211-CE is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211 . This research-use-only ELISA kit can be used for human and animal research purposes worldwide, and has been validated for a wider range of sample types and species.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Serum, Plasma (Citrate). BEK-2211-CE is expressly designed and tested only for use on human blood and plasma samples. Any other use is deemed "off label use" and thus the performance characteristics of the assay will have to be determined by the end user, and such results are not supported by Biosensis or CalBioTech at this time. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
See BEK-2211-1P-CE protocol insert for specific expiration dating of the kit and its components.
Use:
Approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. (www.calbiotech.com) for Biosensis.
This kit is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Reed JL et al. (2021) The effects of high-intensity interval training, Nordic walking and moderate-to-vigorous intensity continuous training on functional capacity, depression and quality of life in patients with coronary artery disease enrolled in cardiac rehabilitation: A randomized controlled trial (CRX study). Prog Cardiovasc Dis. [Epub ahead of print]. Application: Human blood. Valkenborghs SR et al. (2019) Aerobic exercise and consecutive task-specific training (AExaCTT) for upper limb recovery after stroke: A randomized controlled pilot study. Physiother Res Int. [Epub ahead of print]. Application: Human serum.
Specificity:
Human BDNF when used as directed.
Storage:
Store at 2-8°C
Range:
7.8 pg/mL - 500 pg/mL
Sample Type:
Plasma (Citrate),Serum
Sensitivity:
Typical limit of detection (LOD) for BDNF is < 3 pg/mL determined by calculating the mean + 2x standard deviation of mean of blank (n=20).
The Biosensis Mature BDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), pig serum, cell lysates, brain extracts, human milk and Sheep CSF only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples, human milk and CSF. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately ( BL-001-1250 ). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com . This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015). This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance. This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. Extensive validation has demonstrated that the Mature BDNF Rapid TM ELISA shows only minimal cross-reactivity with proBDNF. Please refer to our Technical Note #5 for further details on ELISA assay validation for BDNF isoform detection and quanification. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF ELISA kit (BEK-2211-CE) .
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates, Human Milk, CSF. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Total Number of References: 99 Latest Publications (2019-2022):
Meshkat S et al. (2022) Brain-Derived Neurotrophic Factor (BDNF) as a biomarker of treatment response in patients with Treatment Resistant Depression (TRD): A systematic review & meta-analysis Psychiatry Res. 317:114857 Application: Human, serum. Cook A et al. (2022) Activation of TrkB-Akt signaling rescues deficits in a mouse model of SCA6 Sci Adv. [Epub ahead of print] Application: Mouse, brain extracts. Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Salem HA et al. (2022) Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis Pharmaceuticals. 15, 1012. Application: Rat, brain supernatant. Aldhshan MS & Mizuno TM. (2022) Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice Behav Brain Res. [Epub ahead of print]. Application: Mouse, brain tissue homogenate. Fujino M et al. (2022) Orally Administered Plasmalogens Alleviate Negative Mood States and Enhance Mental Concentration: A Randomized, Double-Blind, Placebo-Controlled Trial Front Cell Dev Biol. 10:894734 Application: Human, plasma. Abrial E et al. (2022) Investigating Predictive Factors of Suicidal Re-attempts in Adolescents and Young Adults After a First Suicide Attempt, a Prospective Cohort Study. Study Protocol of the SURAYA Project Front. Psychiatry. [Epub ahead of print] Application: Human, plasma. Tanaka-Kanegae R et al. (2022) Sufficiently Elevated Core Body Temperature May Be Necessary to Maintain Cerebral Blood Flow Response throughout the Morning Neurosci Med. 13, 70-90 Application: Human, serum. Merlo S et al. (2022) Microglial polarization differentially affects neuronal vulnerability to the ?-amyloid protein: Modulation by melatonin Biochem Pharmacol. 202:115151 Application: Human, cell culture supernatant. Dalile B et al. (2022) Extruded Wheat Bran Consumption Increases Serum Short-Chain Fatty Acids but Does Not Modulate Psychobiological Functions in Healthy Men: A Randomized, Placebo-Controlled Trial Front Nutr. 9:896154 Application: Human, serum. Agapouda A et al. (2022) Rhodiola Rosea Extract Counteracts Stress in an Adaptogenic Response Curve Manner via Elimination of ROS and Induction of Neurite Outgrowth Oxid. Med. Cell. Longev. [Epub ahead of print] Application: Human, cell lysates. Breazeale S et al. (2022) Symptom cluster profiles following traumatic orthopaedic injuries Injury. [Epub ahead of print] Application: Human, serum. Jaehne EJ et al. (2022) Behavioral phenotyping of a rat model of the BDNF Val66Met polymorphism reveals selective impairment of fear memory Transl Psychiatry. 12(1):93 Application: Rat, acid extracted tissue lysates. Wang RY et al. (2022) The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats. Int J Mol Sci. 23, 1780 Application: Rat, brain tissue homogenate and serum. Hugues N et al. (2022) Time-Dependent Cortical Plasticity during Moderate-Intensity Continuous Training Versus High-Intensity Interval Training in Rats. Cereb Cortex. [Epub ahead of print] Application: Rat, cortical tissue homogenate. Cefis M et al. (2021) Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep. 12(1):311 Application: Rat, skeletal muscle tissue homogenate. Becker AM et al. (2021) Acute Effects of Psilocybin After Escitalopram or Placebo Pretreatment in a Randomized, Double-Blind, Placebo-Controlled, Crossover Study in Healthy Subjects. Clin Pharmacol Ther. [Epub ahead of print] Application: Human, plasma. Berbenetz N et al. (2021) The Relationship Between Brain Derived Neurotrophic Factor (BDNF) and Symptoms Following Catheter Ablation for Paroxysmal Atrial Fibrillation (AF)- NEURO-AF Study. Circulation. [Epub ahead of print] Application: Human, serum. Walsh JJ et al. (2021) Short-term ketone monoester supplementation improves cerebral blood flow and cognition in obesity: A randomized cross-over trial. J Physiol. [Epub ahead of print] Application: Human, serum, platelet-poor plasma. Boukhatem I et al. (2021) The brain-derived neurotrophic factor prompts platelet aggregation and secretion. Blood Adv. 5(18):3568-3580 Application: Human, plasma. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765 Application: Human, serum. Inoue T et al. (2021) Ipsilateral BDNF mRNA expression in the motor cortex positively correlates with motor function of the affected forelimb after intracerebral hemorrhage. Brain Res. [Epub ahead of print] Application: Rat, brain homogenate. Shoshina II et al. (2021) Visual processing and BDNF levels in first-episode schizophrenia. Psychiatry Res. [Epub ahead of print] Application: Human, serum. Cappoli N et al. (2021) Effects of remifentanil on human C20 microglial pro-inflammatory activation. Eur Rev Med Pharmacol Sci. 25(16):5268-5274 Application: Human, cell culture. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print] Application: Human, urine. Mori Y et al. (2021) Serum BDNF as a Potential Biomarker of Alzheimer's Disease: Verification Through Assessment of Serum, Cerebrospinal Fluid, and Medial Temporal Lobe Atrophy. Front Neurol. 12:653267 Application: Human, serum. Seno S et al. (2021) Effects of Selective Serotonin Reuptake Inhibitors on Depression-Like Behavior in a Laser-Induced Shock Wave Model. Front Neurol. 12:602038 Application: Mouse, hippocampal homogenates. Medeiros GC et al. (2021) Treatment of depression with ketamine does not change plasma levels of brain-derived neurotrophic factor or vascular endothelial growth factor. J Affect Disord. 280(Pt A):136-139 Application: Human, plasma. Dorandish S et al. (2021) Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media. Int J Mol Sci. 22(13):7059 Application: Human, culture supernatant. Yap NY et al. (2021) Relationship between cytokines and brain-derived neurotrophic factor (BDNF) in trajectories of cancer-related cognitive impairment. Cytokine. [Epub ahead of print] Application: Human, plasma. Wang L et al. (2021) The mediating effect of brain-derived neurotrophic factor levels on childhood trauma and psychiatric symptoms in patients with first-episode schizophrenia. Aust N Z J Psychiatry. [Epub ahead of print] Application: Human. Mallik SB et al. (2021) Remedial effects of caffeine against depressive-like behaviour in mice by modulation of neuroinflammation and BDNF. Nutr Neurosci. [Epub ahead of print] Application: Mouse. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179 Application: Mouse, hippocampal RIPA homogenates. Caruso GI et al. (2021) SIRT1-Dependent Upregulation of BDNF in Human Microglia Challenged with A?: An Early but Transient Response Rescued by Melatonin. Biomedicines. 9(5):466 Application: Human, cell culture supernatant. Miller KM et al. (2021) Striatal Afferent BDNF Is Disrupted by Synucleinopathy and Partially Restored by STN DBS. J Neurosci. 41(9):2039-52 Application: Rat, tissue homogenates (RIPA). Vickneson K et al. (2021) Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia. Diabetologia. 64(6):1436-41 Application: Rat, blood. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Pan S et al. (2021) The microRNA-195 - BDNF pathway and cognitive deficits in schizophrenia patients with minimal antipsychotic medication exposure. Transl Psychiatry. 11(1):117 Application: Human, plasma. Miyamoto T et al. (2021) Effect of pedaling cadence on serum levels of brain-derived neurotrophic factor during ergometric exercise in healthy adults. Sport Sci Health. Application: Human, serum. Normann AJ (2020) The Effect of Light Therapy and Acute Aerobic Exercise on Serum Brain Derived Neurotrophic Factor in Older Adults. MSc Thesis. Application: Human, serum. Holze F et al. (2020) Acute dose-dependent effects of lysergic acid diethylamide in a double-blind placebo-controlled study in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human, plasma. Hasler G et al. (2020) The Association Between Adolescent Residential Mobility and Adult Social Anxiety, BDNF and Amygdala-Orbitofrontal Functional Connectivity in Young Adults With Higher Education. Front. Psychiatry. Application: Human, serum. Wallace AW (2020) The Impact of Six Weeks of Intermittent Fasting, With and Without Aerobic Exercise, on Serum BDNF in Young Adult Males. MSc Thesis. Application: Human, serum. Walsh JJ et al. (2020) The Effect of Exogenous Ketone Monoester Ingestion on Plasma BDNF During an Oral Glucose Tolerance Test. Front Physiol. 11:1094. Application: Human, plasma. Meade GM et al. (2020) A Model of Negative Emotional Contagion Between Male-Female Rat Dyads: Effects of Voluntary Exercise on Stress-Induced Behavior and BDNF-TrkB Signaling. Physiol Behav. 113286 Application: Rat, serum. Hutten NRPW et al. (2020) Low Doses of LSD Acutely Increase BDNF Blood Plasma Levels in Healthy Volunteers. ACS Pharmacol. Transl. Sci. Application: Human, plasma. Barbosa AC et al. (2020) Assessment of BDNF serum levels as a diagnostic marker in children with autism spectrum disorder. Sci Rep. 10(1):17348. Application: Human, serum. Okamura M et al. (2020) Low-Level Inhibition of GABAergic Synapses Enhances Gene Expressions Crucial for Neuronal Plasticity in the Hippocampus After Ischemic Stroke. J Stroke Cerebrovasc Dis. 29(12):105316. Application: Rat, hippocampus homogenate. Payne AJ et al. (2020) The Effects of Alcohol on BDNF and CD5 Dependent Pathways. PhD Thesis. Application: Mouse, RIPA tissue homogenate. Nishiyama M et al. (2020) Homostachydrine is a Xenobiotic Substrate of OCTN1/SLC22A4 and Potentially Sensitizes Pentylenetetrazole-Induced Seizures in Mice. Neurochem Res. [Epub ahead of print]. Application: Mouse, acid-extracted hippocampal homogenate. Lorinczova HT et al. (2020) Co-Administration of Iron and a Bioavailable Curcumin Supplement Increases Serum BDNF Levels in Healthy Adults. Antioxidants (Basel). 9(8):E645. Application: Human serum. Yap NY F et al. (2020) Associations of plasma brain-derived neurotrophic factor (BDNF) and Val66Met polymorphism (rs6265) with long-term cancer-related cognitive impairment in survivors of breast cancer. Breast Cancer Res Treat. [Epub ahead of print]. Application: Human plasma. Vasilopoulou F et al. (2020) Amelioration of BPSD-Like Phenotype and Cognitive Decline in SAMP8 Mice Model Accompanied by Molecular Changes After Treatment With I 2-Imidazoline Receptor Ligand MCR5. Pharmaceutics. 12(5):E475. Application: Mouse hippocampus RIPA-homogenates. Mueller ST et al. (2020) Negative Association Between Left Prefrontal GABA Concentration and BDNF Serum Concentration in Young Adults. Heliyon. 6(5):e04025. Application: Human serum. Chen LF et al. (2020) The NMDA receptor subunit GluN3A regulates synaptic activity-induced and myocyte enhancer factor 2C (MEF2C)-dependent transcription. J Biol Chem. [Epub ahead of print]. Application: Rat neuronal cell lysate, acid-extracted. Companys-Alemany J et al. (2020) A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice. Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Furukawa Y et al. (2020) Citrus Auraptene Induces Expression of Brain-Derived Neurotrophic Factor in Neuro2a Cells. Molecules. 25(5). Application: Mouse Neuro2a culture supernatant. Holze F et al. (2019) Distinct acute effects of LSD, MDMA, and D-amphetamine in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human plasma. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human plasma. Sartori A et al. (2019) Interferon-beta, but not Glatiramer Acetate treatment induces gender-specific increase in BDNF serum levels in relapsing-remitting multiple sclerosis female patients. Res J Neuro N Disord. 1:5-18. Application: Human serum. Please refer to our Technical Note #5 for validation experiments disproving the author's claim that the Biosensis Mature BDNF Rapid TM ELISA quantifies total BDNF! Vanicek T et al. (2019) Repetitive Enhancement of Serum BDNF Subsequent to Continuation ECT. Acta Psychiatr Scand. [Epub ahead of print]. Application: Human serum. Gejl AK et al. (2019) Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy. Sci Rep. 9(1):9655. Application: Human serum and EDTA-plasma. Li X et al. (2019) Exercise enhances the expression of brain-derived neurotrophic factor in the hippocampus accompanied by epigenetic alterations in senescence-accelerated mice prone 8. Neurosci Lett. [Epub ahead of print]. Application: Mouse brain homogenates. Yang CY et al. (2019) Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats. Neurotox Res. [Epub ahead of print]. Application: Rat brain homogenates. Du Y et al. (2019) Genome-Wide, Integrative Analysis Implicates Exosome-Derived MicroRNA Dysregulation in Schizophrenia. Schizophr Bull. [Epub ahead of print]. Application: Human serum. Vanicek T et al. (2019) Acute and Subsequent Continuation Electroconvulsive Therapy Elevates Serum BDNF Levels in Patients with Major Depression. Brain Stimul. [In press]. Application: Human serum, plasma. Duart-Castells L et al. (2019) 7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway. Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates.
Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value.
Cross Reactivity:
No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM). Additional proBDNF cross-reactivity experiments were conducted as summarized in our <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/Technical-Note-5-Mature-BDNF-Isoform-Detection-and-Quantification-by-ELISA.pdf">Technical Note #5</a>.
Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis. Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Product Type:
Buffer & Reagent
Format:
Powder
Applications:
IP,WB
Application Details:
Please download the protocol below for detailed instructions on how to use Phospho-Sure in neuronal and other soft tissues.
Alternative Names:
Phospho-sure
Biosensis Brand:
Phospho-Sure RTD
Shelf Life:
Powdered format can be stored up to 12 months after purchase under cool, dry conditions.
Use:
For research use only.
Product references:
1. Suneja SK, Mo Z, Potashner SJ. (2006) Phospho-CREB and other phospho-proteins: improved recovery from brain tissue. J Neurosci Methods. 2006 Jan 30;150(2):238-41. 2. Elvira Mass, Dagmar Wachten, Anna C. Aschenbrenner, Andre Voelzmann, Michael Hochemail (2014) Murine Creld1 Controls Cardiac Development through Activation of Calcineurin/NFATc1 Signaling, Developmental Cell Volume 28, Issue 6, p711-726
Storage:
The dry, unopened container should be stored at room temperature in a dry or desiccated location protected from light. Do not store in the refrigerator unless material is in a dry, moisture free environment. Material is hydroscopic so once the seal is broken it should be hydrated and not resealed while dry. Once hydrated, the buffer can be stored at 2-8°C for up to 3 months. Solution can be frozen but clumping may occur upon thawing and is not recommended.
Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis. Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.
Product Type:
Buffer & Reagent
Format:
Powder
Applications:
IP,WB
Application Details:
Please download the protocol below for detailed instructions on how to use Phospho-Sure in neuronal and other soft tissues.
Alternative Names:
Phospho-sure
Biosensis Brand:
Phospho-Sure RTD
Shelf Life:
Powdered format can be stored up to 12 months after purchase under cool, dry conditions.
Use:
For research use only.
Product references:
1. Suneja SK, Mo Z, Potashner SJ. (2006) Phospho-CREB and other phospho-proteins: improved recovery from brain tissue. J Neurosci Methods. 2006 Jan 30;150(2):238-41. 2. Elvira Mass, Dagmar Wachten, Anna C. Aschenbrenner, Andre Voelzmann, Michael Hochemail (2014) Murine Creld1 Controls Cardiac Development through Activation of Calcineurin/NFATc1 Signaling, Developmental Cell Volume 28, Issue 6, p711-726
Storage:
The dry, unopened container should be stored at room temperature in a dry or desiccated location protected from light. Do not store in the refrigerator unless material is in a dry, moisture free environment. Material is hydroscopic so once the seal is broken it should be hydrated and not resealed while dry. Once hydrated, the buffer can be stored at 2-8°C for up to 3 months. Solution can be frozen but clumping may occur upon thawing and is not recommended.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Fusion protein of human IgG Fc fragment.
Applications:
WB,ELISA
Additional Info:
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Clone number:
EM-07
Antibody Isotype:
IgG1
Application Details:
Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Fusion protein of human IgG Fc fragment.
Applications:
FC
Additional Info:
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgG.
Applications:
FC,WB,ELISA
Additional Info:
The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG.
s Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of primary antibody in WB and effectively increase storage time of the primary antibody working solution.
Product Type:
Buffers & Mixes
Storage Temp:
Store at 4°C for one year. Avoid multiple freeze-thaw cycles.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
evGAG is a patented purification method that allows isolation of extracellular vesicles (EVs) from biofluids. The evGAG reaction is based on the interaction between the precipation solution and glycosaminoglycans (GAGs) in the EVs.The product is ideal for the discovery of EV associated biomarkers.
- Suitable for human and animal biofluids - Rapid and simple process
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Enolase kit
Storage Temp:
+ 4 C
Additional Info:
No initial exosome purification required User friendly and reliable and efficient Suitable for multiple marker analyses Available in custom format
HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
No initial exosome purification required User friendly and reliable and efficient Suitable for multiple marker analyses Available in custom format
HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
No initial exosome purification required User friendly and reliable and efficient Suitable for multiple marker analyses Available in custom format
The ExoTEST Ready to Use Kit is the ideal platform for capturing and quantifying exosomes from biological samples. It features ELISA plate(s) pre-coated with primary antibodies to exosome-specific markers, plus the secondary antibody, substrate reagents and the exosome standards. Plus we can also help you customise your own kit by choosing from the individual reagents available in our catalog.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
ExoTEST has been developed to fill the need for a reliable method to quantify exosomes and examine their biomarkers. >>>> HansaBioMed also provide the ability to design and create your own kit by choosing from among the wide variety of reagents available in our catalog. 1-Select the exosome standard you need. 2-Select the primary antibody for exosome detection.
HBM provides a ready to use kit for the qualitative FACS analysis of exosomes. The Exo-FACS assay for exosome characterisation consists of 4um beads used for the direct overall capture of pre-purified, validated and lyophilized exosomes from cell culture supernatants or human biological fluids. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
FACS analysis
Storage Temp:
+ 4 C
Applications:
FACS Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.
ExoTEST has been developed to fill the need for a reliable method to quantify exosomes and examine their biomarkers. >>>> HansaBioMed also provide the ability to design and create your own kit by choosing from among the wide variety of reagents available in our catalog. 1-Select the exosome standard you need. 2-Select the primary antibody for exosome detection.
Up to 100 ul of biological sample can be loaded per well.
Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.
Assay type:
Sandwich enzyme-linked immunoassay.
Sensitivity:
The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Background Info:
Sample type: Cell culture supernatant.
Starting material: Up to 100 ul of biological sample can be loaded per well. Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma, serum, urine and cell culture supernatant. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 murine antibody.
Exosome standard: Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: kit contains exosome standards for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated serum sample can be directly used for capture.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine.
ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit.
Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody
Exosome standard: Lyophilized exosomes from serum of healthy donors (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples. HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custommade kits in order to meet the need for detection of specific exosome markers.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Background Info:
Sample type: Urine
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine.
ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit.
Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody
Exosome standard: Lyophilized exosomes from urine of healthy donors (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Background Info:
Sample type: Plasma
Starting material: Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Assay type: Sandwich enzyme-linked immunoassay.
Sensitivity: The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.
Product overview: ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine.
ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely ?-CD9. Secondary antibodies and substrate reagents are included in the kit.
Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.
ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.
Primary antibody: ?-CD9 mouse antibody
Exosome standard: Lyophilized exosomes from plasma of healthy donors (see details).
Assay protocol: Kit contains all the reagents necessary for exosome capture and protein targeted detection.
Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.
This standards enable both absolute and relative quantification exosomes in analyzed samples.
Product Type:
immunoplate
Storage Temp:
+ 4 C
Applications:
Exosome capture and quantification
Additional Info:
Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples.
Application Details:
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
TFF-MV is a filter cartridge in hollow fibers made of polysulfone. The filter is very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.
Filter cartridge: Polysulfone hollow fibres
Sample volume per reaction: Recommended sample volume from 10 ml up to several liters if connected to mechanical pump
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles for. TFF-EVs allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5ml) or connected with a mechanical system for purifying larger volumes.
TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit of the TFF technique which is usable for processing big volumes of fluids. The filter is additionally suitable for dialyzing EV preparations.
TFF-Easy can be easily washed and it is reusable multiple times.
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles for. TFF-EVs allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5ml) or connected with a mechanical system for purifying larger volumes.
TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit of the TFF technique which is usable for processing big volumes of fluids. The filter is additionally suitable for dialyzing EV preparations.
TFF-Easy can be easily washed and it is reusable multiple times.
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Concentrator | EV purification
Storage Temp:
+ 4 C
Applications:
Concentrator | EV purification
Additional Info:
TFF-MV is a filter cartridge in hollow fibers made of polysulfone. The filter is very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.
Filter cartridge: Polysulfone hollow fibres
Sample volume per reaction: Recommended sample volume from 10 ml up to several liters if connected to mechanical pump
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Concentrator | EV purification
Storage Temp:
+ 4 C
Applications:
Concentrator | EV purification
Additional Info:
TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit of the TFF technique which is usable for processing big volumes of fluids. The filter is additionally suitable for dialyzing EV preparations.
TFF-Easy can be easily washed and it is reusable multiple times.
Applications: Concentration of diluted matrices as cell media or urine prior to EV isolation. Easy removal of small molecules and ions from the EV preparation. EV dialysis for changing buffer conditions. High efficiency of EV isolation if coupled with SEC columns.
Advantages: Washable, reusable multiple times, easy to use, fast concentration of EV containing matrices. EV dialysis.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from cell supernatants of human cell lines. Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Concentrated cell culture supernatant samples (10X in spin concentrator) are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Immunobeads are coupled with antibodies that allows for exosome isolation from human biofluids (tested for plasma, serum, urine). Latex immunobeads can be easily recovered after incubation using a centrifuge. Exosomes captured on immunobeads can be used directly protein and mRNA/miRNA profiling.
Starting material: Recommended starting sample volume from 0.5ml of sample up to 1ml. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Our Immunoplates are designed for the capture of exosomes from any biological sample. Our plates are a standard 96-well format allowing assays to be conducted as singles and/or multi-well. This enables easy optimisation of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). Uncoated and/or covalently-coated plates are also available if required.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
EXO-Prep is a new and efficient method for exosome isolation from biofluids (i.e plasma serum and urine) and from cell culture supernatants. Exosomes are quicky isolated from your sample by an incubation step with EXO-Prep precipitation reagent. Exosome pellet is subsequently collected by one-step centrifugation and can be easily resuspended in PBS 1X. Solubilized exosomes are suitable for different downstream analyses such as proteomic or nucleic acids marker profiling, NTA, electron microscopy etc.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Store up to 1 year at 4°C >>> Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.. Avoid repeated freeze-and-thaw cycles.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized plant derived ELNs
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized plant derived ELNs
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized plant derived ELNs
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized plant derived ELNs
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized plant derived ELNs
Storage Temp:
Store up to 6 months years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Purified and lyophilized Microvesicles (MV) are obtained from cell conditioned media. Diff erent EVs are separated by size using tangential flow filtration (TFF) and subsequantly purified by size exclusion chromatography (SEC) and microfiltration. Isolated vesicles are quantifi ed and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix).
Product Type:
Lyophilized microvesicles
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted microvesicles: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Quantity per vial: 100 µg (number of particles in 100 µg: > 1x1010
Fluorescent label type: Membrane lipid dye (penetrates into exosome membrane).
Spectrum data: Excitation maximum from 500 nm to 650 nm. Emission maximum from 510 to 665 nm.
Available Options: Plasma | urine | serum | A549 cell line | B16F10 mouse cell line | BLCL cell line | BPH-1 cell line | COLO-1 cell line| DAUDI cell line | HCT116 cell line | K-562 cell line | PC3 cell line | SK-N-SH cell line | U87MG cell line
Application Details:
Fluorescence microscopy. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Quantity per vial: 100 µg (number of particles in 100 µg: > 1x1010
Fluorescent label type: Membrane lipid dye (penetrates into exosome membrane).
Spectrum data: Excitation maximum from 500 nm to 650 nm. Emission maximum from 510 to 665 nm.
Available Options: Plasma | urine | serum | A549 cell line | B16F10 mouse cell line | BLCL cell line | BPH-1 cell line | COLO-1 cell line| DAUDI cell line | HCT116 cell line | K-562 cell line | PC3 cell line | SK-N-SH cell line | U87MG cell line
Application Details:
Fluorescence microscopy. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Storage of lyophilized exosomes: Store up to 1 years at 4°C (Expire date indicated on the label). Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.
Application Details:
NTA in fluorescent mode Flow cytometry. Cell spike- in and in vitro tracking Fluorescence assay calibration EV uptake monitoring, assay and instrument calibration
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Chinese Hamster Ovary (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Chinese Hamster Ovary (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Chinese Hamster Ovary (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Chinese Hamster Ovary (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from MSC from human adipose tissue (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from MSC from human adipose tissue (for research use only)
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
Our purified lyophilized exosomes are obtained from different biological sources including cell culture supernatant, human plasma, serum and urine. Isolation is performed by a combination of ultracentrifugation and microfiltration procedures, and subsequent quantification/validation for overall protein content and particle number by NTA with Nanosight.
Background Info:
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Product Type:
Lyophilized exosomes
Storage Temp:
Store up to 3 years at 4°C >>> Storage of reconstituted exosomes: -20°C for up to one month or -80°C for up to 6 months. Avoid repeated freeze-and-thaw cycles.
Lyophilized Exosome Standards from Human Biofluids
Additional Info:
Lyophilization is an ideal method for long-term storage of exosomes and microvesicles. It does not alter the stability of exosome proteins and nucleic acids, in comparison to other storage methods, including storage of fresh EVs at -20°C. Lyophilized EVs and microvesicles are easy to ship and stable for long term storage (up to 36 months).
Application Details:
Assay calibration. Control (spike-in) for exosome quantification. Protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
