Anti-p53 (Clones PAb1620 and PAb122)

Anti-p53 (Clones PAb1620 and PAb122)

p53 acts as a tumor suppressor in many tumor types; depending on the physiological circumstances and cell type it induces growth arrest or apoptosis. Involved in cell cycle regulation as a trans-activator, p53 acts to negatively regulate cell division by controlling a set of genes required for this process. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

p53 localizes in the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through post-translational modifications, including phosphorylation and acetylation.

Our p53 antibodies for flow cytometry.

Anti p53 Clone PAb122 binds to amino acids 371 - 378 of p53 and is recommended for the detection of a conserved denaturation-resistant determinant of the p53 protein.

Anti p53 Clone PAb1620 binds a conformational epitope that disappears in some mutant p53 or upon denaturation. It therefore preferentially immunoprecipitates wild-type p53 and does not cause immunoprecipitation of mutant and denatured p53.

Caprico Biotechnologies' antibodies are specific for human immune cells, and can be used in both research and diagnostic applications.