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Product Search Results: SMC-176D

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Product Search Results: SMC-176D

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StressMarq SMC-176D 100 µg £263.00
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Product Detail: This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Product Type: Antibodies
Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temp: -20ºC
Host Animal: Mouse
Species Reactivity: Mm | Fs | Av | Am | Shk
Immunogen: Bovine Rhodopsin
Applications: WB | IHC | ICC/IF | IP | ELISA
Clone number: 4D2
Antibody Isotype: IgG1
Application Details: WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.
Research Area(s): Neuroscience | Neurotransmitter Receptors | Rhodopsins | Cell Signaling
Target: Rhodopsin
Conjugate: Unconjugated
Alternative Name(s): OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody
Category: Monoclonal
Immunogen Species: Bovine
Accession Number: NP_001014890.1
Gene ID: 509933
Swiss-Prot: P02699
Species Reactivity Full Name: Mammals | Fish | Anchovies | Avian | Amphibians | Shark | Extinct Spiny Shark (Acanthodes bridgei)
Purification: Protein G Purified
Concentration: 1 mg/ml
Specificity: Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Certificate of Analysis: 1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization: Membrane
References: 1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
Tarriff Code: 3002.15.0000
ADR Code: Non-hazardous
UN Code for transport: Non-hazardous
Country of Origin: Canada
Cite this Product: StressMarq Biosciences Cat# SMC-176D, RRID: AB_10599810