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Product Search Results: SMC-155D

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Product Search Results: SMC-155D

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StressMarq SMC-155D 100 µg £243.00
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Product Detail: This antibody is available conjugated to the following 23 tags: AP (Alkaline Phosphatase), HRP (Horseradish peroxidase), Biotin, Streptavidin, Dylights 350, 405, 488, 594, 633, APC/Cy7; ATTO 390, ATTO 488, ATTO 565, ATTO 594, PE/ATTO594, ATTO633, ATTO 655, ATTO 680, ATTO700, FITC, R-PE, APC, PerCP. Please call for pricing.
Background Info: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
Product Type: Antibodies
Format: PBS, 50% glycerol, 0.09% sodium azide
Storage Temp: -20ºC
Host Animal: Mouse
Species Reactivity: ALL
Immunogen: 8-hydroxy-guanosine-BSA and –casein conjugates
Applications: IHC | ICC/IF | ELISA | DB | IP | FCM | FA
Clone number: 15A3
Antibody Isotype: IgG2b
Application Details: IHC (1:1000); optimal dilutions for assays should be determined by the user.
Research Area(s): Cancer | Oxidative Stress | Cell Signaling | Post-translational Modifications | Oxidation | Cell Signaling | Epigenetics and Nuclear Signaling | DNA/RNA | DNA Damage and Repair
Target: DNA Damage
Conjugate: Unconjugated
Alternative Name(s): 8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, 8OHG Antibody, 80G Antibody, 8 hydroxyguanine Antibody, 8 hydroxy 2' deoxyguanosine Antibody, 8 hydroxyguanosine Antibody, 8 OHG Antibody, 8-OHG Antibody, 8OHdG Antibody
Category: Monoclonal
Trademark: MOLECULAR SIGNATURE®
Species Reactivity Full Name: Species Independent
Purification: Protein G Purified
Concentration: 1 mg/ml
Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
References: 1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241.
2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15.
3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432.
4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283.
5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9.
6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9.
7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.
Tarriff Code: 3002.15.0000
ADR Code: Non-hazardous
UN Code for transport: Non-hazardous
Country of Origin: Canada
Cite this Product: StressMarq Biosciences Cat# SMC-155D, RRID: AB_889490