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Product Search Results: M-1752-100

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Product Search Results: M-1752-100

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Biosensis M-1752-100 100 µg £288.00
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Product Detail: Mouse anti-Cellular oncogene fos (c-Fos) Monoclonal Antibody (Unconjugated), suitable for IHC-Frozen, ICC.
Background Info: FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).
Product Type: Antibody
Antibody Type: Monoclonal
Format: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal: Mouse
Species Reactivity: Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen: Full length, E.coli-derived recombinant human c-FOS protein.
Applications: ICC,IHC-Frozen
Clone number: 2H2
Antibody Isotype: IgG1
Application Details: Immunohistochemistry (IHC) and immunocytochemistry (ICC): 1-2 µg/mL. This antibody has been shown to work on 4% PFA fixed mouse brain sections.<br><br>Western blotting (WB): 0.5-1.0 µg/mL. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names: Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
Biosensis Brand: Biosensis®
Conjugate: Unconjugated
Shelf Life: 12 months after date of receipt (unopened vial).
Use: For research use only.
Product references: Choi S et al. (2020). Parallel ascending spinal pathways for affective touch and pain. Nature. 587(7833):258-263. Application: IHC . Species: Mouse . Bai L et al. (2019). Genetic Identification of Vagal Sensory Neurons That Control Feeding. Cell. 179(5):1129-43. Application: IHC . Species: Mouse .
Specificity: Human. Horse, cow, pig, chicken, rat, mouse.
Storage: Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification: Affinity purified.
Target: Cellular oncogene fos (c-Fos)
Uniprot Number: P01100