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ImmunoStar 24274 100uL £248.00
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Product Detail: 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
Background Info: Raised to 5-HIAA coupled to BSA with paraformaldehyde.
Product Type: Antibody - Antibodies
Antibody Type: polyclonal
Format: Lyophilised
Host Animal: Rabbit
Species Reactivity: Rat
Immunogen: 5-HIAA
Applications: Immunohistochemistry, Immunocytochemistry, immunofluorescence
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: The 5-HIAA antibody was raised to 5-HIAA coupled to BSA with paraformaldehyde. The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique.

The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.