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5-HT (Serotonin) Rabbit Antibody ImmunoStar 20080 100uL £297.00
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Product Detail: 5-HT (Serotonin) Rabbit Antibody
Background Info: Raised against serotonin coupled to BSA with paraformaldehyde.
Product Type: Antibody - Antibodies
Antibody Type: polyclonal
Format: Lyophilised
Host Animal: Rabbit
Species Reactivity: Rat, Mouse, Aplysiid Molluscs
Immunogen: Serotonin
Applications: Immunohistochemistry, Immunocytochemistry, immunofluorescence
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Both our 5-HT Goat and Rabbit Antibodies were raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA.

Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.
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