The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/600 dilution and a proven biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala.
Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.
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