Androgen receptor (AR) is a member of the steroid receptor superfamily that is essential for the growth of prostate cancer cells. Ithas been reported that tyrosine phosphorylation of AR is induced by growth factors and elevated in hormone-refractory prostate tumors. Data suggest that growth factors and their downstream tyrosinekinases, which are elevated during hormone-ablation therapy, can induce tyrosine phosphorylation of AR. Such modification may be important for prostate tumor growth under androgen-depletedconditions. Cellular signaling occurs following androgen binding tothe AR and translocation to the nucleus. This activated complex associates with androgen-responsive elements contained in the DNAsequence of target genes, affecting the transcriptional activity of these genes.
Antibody Isotype:
IgG
Monosan Range:
MONOSAN
Clone:
EP120
Concentration:
n/a
Storage buffer:
Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide
Storage:
2-8°C
References 1:
Horie K, et al.Hum Reprod, 1992, 7:1461-1466
References 2:
Loda M, et al.: Mod Pathol, 1994, 7:388-391
References 3:
Miyamoto H, et al.: Prostate, 2004, 61:332-353
References 4:
Guo Z, et al.: Cancer Cell, 2006, 10:309-319
References 5:
Callewaert L, et al.: Biochem Biophys Res Commun, 2003, 306:46-52
The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. </br> Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.</br> The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA, in frozen tissue sections fixed with acetone and in flow cytometry using a cell permeabilization method.
Antibody Isotype:
IgG1
Monosan Range:
MONOSAN
Clone:
8F4
Concentration:
100 ug/ ml
Storage buffer:
PBS with 0.1% BSA and 0.02% sodium azide
Storage:
2-8°C
References 1:
Huugen; D et al. Am J Pathol 2005; 167: 47
References 2:
Wang, D et al Blood 2006, 108:1223
References 3:
Matthijsen; M et al. Am J Pathol 2007; 171: 1743
References 4:
Leeuwen van M et al. Arterioscler Thromb Vasc Biol 2008; 28: 84
The histochemical antibody for Neurokinin 3 Receptor is generated in a rabbit from a synthetic peptide corresponding to rat NK3R 438-452 coupled to carrier protein. The antiserum is provided as l00 µL of affinity purified liquid.
Product Type:
Antibody - Antibodies
Antibody Type:
polyclonal
Format:
Liquid
Host Animal:
Rabbit
Species Reactivity:
Rat
Expected Species:
100% sequence homology with rat, human, mouse, dog and gerbil
Immunogen:
Synthetic peptide corresponding to rat NK3R 438-452 coupled to carrier protein.
The NK3R antiserum was quality control tested using standard immunohistochemical methods in rat hypothalamus using biotin/avidin-HRP techniques.Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK3R peptide residues 438-452. Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 80 and 115 kD.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 4 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Plasmodium falciparum
Immunogen:
Ferredoxin purified from Malaria parasite, Plasmodium falciparum, UniProt:Q8IED5
Applications:
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
18 kDa
Selected references:
Kimata and Ariga et al. (2007). Cloning and Characterization of Ferredoxin and ferredoxin-NADP+ Reductase From Human Malaria Parasite. J Biochem. 141(3):421-8. doi: 10.1093/jb/mvm046. Kabayashi et al. (2007). Mitochondria and Apicoplast of Plasmodium Falciparum: Behaviour on Subcellular Fractionation and the Implication. Mitochondrion 7(1-2):125-32. doi: 10.1016/j.mito.2006.11.021.
The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on subpopulation of T lymphocytes. CD20 is expressed widely in the large majority of cases of B-cell leukaemia and lymphoma. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on subpopulation of T lymphocytes. CD20 is expressed widely in the large majority of cases of B-cell leukaemia and lymphoma. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
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