| Product Detail: |
This protein assay, based on the method of Bradford, is a simple and accurate procedure for determining protein concentration.
The Bradford Assay is a dye-binding assay in which a differential color change of a dye occurs in response to various concentrations of protein. The absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 dye shifts from 465 nm to 595 nm when binding to protein. The Coomassie blue dye binds to primarily basic and aromatic amino acid residues, especially arginine. Protein extraction and sample dilution buffer are included in the kit to avoid interactions with buffer and detergent components. The diluted, ready-to-use BSA standards are also included with the kit.
BENEFITS:-
Fast extraction completed in 560 min
Vesatile - compatible with commonly used detergents and reducing agents
High recovery rate (75 - 95%)
High reproducibility
Complete kit contains extraction & sample dilution buffer & ready-to-use protein standards
Economical |
| Background Info: |
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Protein Detection - Protein Quantitation |
| Additional Info: |
MEASUREMENT: microtiter plate reader (595 nm)
TESTS PER KIT: The kit has the capacity for 1000 determinations of protein concentration in the 96-well format.
SHELF LIFE: 12 months when stored under optimal conditions
SAMPLE SIZE: 20 uL/well
SAMPLE TYPES: cells, serum and tissue |
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| Product Detail: |
The MaxDiscovery In Cell ELISA Kit is an enzyme immunoassay for quantitative protein analysis directly in adherent cell culturesthere is no need to lyse cells or run protein gels. The technique can be thought of as an ELISA performed on fixed, permeablized cells rather than on immobilized antigen. Like most ELISA assays, protein is detected using a series of two antibodies, a primary antibody for the protein of interest (not provided with the kit) and an enzyme-conjugated secondary antibody. The protein:antibody complex is visualized by adding an enzyme substrate that, when catalyzed, changes the color of the assay solution within the well of the tissue culture plate so that it can be read in a spectrophotometer at 450 nm. The relative amount of protein in the cells will be directly proportional to the amount of signal that is obtained, making this a simple, convenient, and accurate technique for quantifying specific protein levels within cell lines.
The MaxDiscovery In Cell ELISA Kit contains materials for washing, fixing, and permeablizing cells in culture. HRP-conjugated anti-mouse and anti-rabbit secondary antibodies are included in the kit so that user-supplied primary antibodies from either mouse or rabbit may be used for detection of the protein of interest. In addition, the kit includes mouse-derived antibody targeting glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is one of the key enzymes involved in glycolysis, and because of its high-level, and constitutive expression, it is widely used as a loading control for Northern/Western blots and for protein normalization. The quantity of GAPDH in a sample is directly proportional to the number of cells in the sample. GAPDH expression is often used as an internal control in gene expression studies. Typically, GAPDH expression is used to normalize expression signal from the gene-of-interest with respect to cell number. This has been particularly useful for RNAi studies or other drug treatment studies. The MaxDiscovery In Cell ELISA assay makes it easy to quickly measure levels of GAPDH (positive control) or your protein-of-interest directly in cultured cells for relative quantitative analysis.
BENEFITS:-
Simplifies analysis No need to remove cells from cell growth plate
Fast Results in 3 hrs instead of 12-24 hrs
Scalable
Ideal for high-throughput & automation
Ideal for siRNA studies and drug screening
Complete kit - Contains GAPDH control |
| Background Info: |
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans. |
| Product Type: |
Protein Detection - Protein Quantitation |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Additional Info: |
FORMAT: 1 x 96 well with adherent cells
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit includes enough reagents for 2 x 96 well plates.
SHELF LIFE: 12 months when stored under optimal conditions |
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