Search Results : NOVA-4247-05 Num PRODUCTS MATCHED : 1

Product - please click for more details Brand Cat No Size Price (£)
NEXTflex™ Periodic Fever-1 Amplicon Panel for Illumina platforms (Barcodes 193 -288) Bioo Scientific NOVA-4247-05 96 rxns £4669.00
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Product Detail: The NEXTflex™ Periodic Fever-1 Amplicon Panel allows you to quickly target and sequence 27 exons of the TNFRSF1A, NLRP3, and MVK loci. This kit contains primer pairs and reagents needed to amplify these coding regions from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTflex Periodic Fever-1 Amplicon Panel has the capability of multiplexing up to 360 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.


A Complete Solution for Targeted TNFRSF1A, NLRP3, and MVK Sequencing

The NEXTflex Periodic Fever-1 Amplicon Panel is a complete, validated solution that targets 27 exons in the TNFRSF1A, NLRP3, and MVK loci using 59 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Periodic Fever-1 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.



[BENEFITS]
•100% coverage of TNFRSF1A, NLRP3, and MVK exons and flanking intron-exon boundaries
•97% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
•384 unique barcodes available
•Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
•Low input - Only 20 ng DNA isolated from fresh or frozen samples required
Product Type: NGS - Illumina Amplicon Panels
Applications: Periodic Fever-1 Amplicon Panel
Search Terms: Illumina Periodic Fever-1 Amplicon Panel
Application Details: Please use our contact form above to request a copy of the datasheet
Catalogue Number Update: Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 4247-05
NGS Applications: Periodic Fever-1 Panel